Human Gene RPSA (ENST00000458478.6) from GENCODE V44
Description: Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA- precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria. (from UniProt P08865) RefSeq Summary (NM_002295): Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]. Gencode Transcript: ENST00000458478.6 Gencode Gene: ENSG00000168028.15 Transcript (Including UTRs) Position: hg38 chr3:39,406,733-39,412,438 Size: 5,706 Total Exon Count: 7 Strand: + Coding Region Position: hg38 chr3:39,407,654-39,412,368 Size: 4,715 Coding Exon Count: 6
ID:RSSA_HUMAN DESCRIPTION: RecName: Full=40S ribosomal protein SA; AltName: Full=37 kDa laminin receptor precursor; Short=37LRP; AltName: Full=37/67 kDa laminin receptor; Short=LRP/LR; AltName: Full=67 kDa laminin receptor; Short=67LR; AltName: Full=Colon carcinoma laminin-binding protein; AltName: Full=Laminin receptor 1; Short=LamR; AltName: Full=Laminin-binding protein precursor p40; Short=LBP/p40; AltName: Full=Multidrug resistance-associated protein MGr1-Ag; AltName: Full=NEM/1CHD4; FUNCTION: Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA- precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria. SUBUNIT: Monomer (37LRP) and homodimer (67LR). Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (28S, 5.8S and 5S). Interacts with RPS21. Interacts with several laminins including at least LAMB1. Interacts with MDK (By similarity). Interacts with PRNP. The mature dimeric form interacts with PPP1R16B (via its fourth ankyrin repeat). Interacts with PPP1CA only in the presence of PPP1R16B. SUBCELLULAR LOCATION: Cell membrane. Cytoplasm. Nucleus (By similarity). Note=67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B in the cell membrane. PTM: Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association. PTM: Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions (By similarity). MISCELLANEOUS: This protein appears to have acquired a second function as a laminin receptor specifically in the vertebrate lineage. MISCELLANEOUS: It is thought that in vertebrates 37/67 kDa laminin receptor acquired a dual function during evolution. It developed from the ribosomal protein SA, playing an essential role in the protein biosynthesis lacking any laminin binding activity, to a cell surface receptor with laminin binding activity. SIMILARITY: Belongs to the ribosomal protein S2P family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P08865
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
BC070263 - Homo sapiens ribosomal protein SA, mRNA (cDNA clone MGC:88252 IMAGE:3996864), complete cds. JD263087 - Sequence 244111 from Patent EP1572962. AK129928 - Homo sapiens cDNA FLJ26418 fis, clone KDN00506, highly similar to 40S ribosomal protein SA. BC107567 - Homo sapiens ribosomal protein SA, mRNA (cDNA clone MGC:104342 IMAGE:3681016), complete cds. BC066941 - Homo sapiens ribosomal protein SA, mRNA (cDNA clone MGC:87296 IMAGE:4820432), complete cds. D28372 - Homo sapiens mRNA for laminin binding protein, 5'UTR region. BC062714 - Homo sapiens ribosomal protein SA, mRNA (cDNA clone MGC:72006 IMAGE:3686924), complete cds. BC005391 - Homo sapiens ribosomal protein SA, mRNA (cDNA clone MGC:12521 IMAGE:3997019), complete cds. AF503367 - Homo sapiens multidrug resistance-associated protein MGr1-Ag mRNA, complete cds. BC013827 - Homo sapiens ribosomal protein SA, mRNA (cDNA clone MGC:17122 IMAGE:3446816), complete cds. BC050688 - Homo sapiens ribosomal protein SA, mRNA (cDNA clone MGC:60265 IMAGE:4443949), complete cds. X15005 - Human mRNA for potential laminin-binding protein (nem/1cHD4). BC034537 - Homo sapiens ribosomal protein SA, mRNA (cDNA clone MGC:17506 IMAGE:3455545), complete cds. BC053370 - Homo sapiens ribosomal protein SA, mRNA (cDNA clone MGC:61620 IMAGE:5108337), complete cds. BC071968 - Homo sapiens ribosomal protein SA, mRNA (cDNA clone MGC:88664 IMAGE:5814758), complete cds. BC010418 - Homo sapiens ribosomal protein SA, mRNA (cDNA clone MGC:16557 IMAGE:4079845), complete cds. BC071693 - Homo sapiens ribosomal protein SA, mRNA (cDNA clone MGC:87940 IMAGE:3884457), complete cds. BC018867 - Homo sapiens ribosomal protein SA, mRNA (cDNA clone IMAGE:3141130). BC071969 - Homo sapiens ribosomal protein SA, mRNA (cDNA clone MGC:88665 IMAGE:6483856), complete cds. BC073863 - Homo sapiens ribosomal protein SA, mRNA (cDNA clone MGC:90285 IMAGE:3924066), complete cds. J03799 - Human colin carcinoma laminin-binding protein mRNA, complete cds. BC071970 - Homo sapiens ribosomal protein SA, mRNA (cDNA clone MGC:88666 IMAGE:6484091), complete cds. BC068062 - Homo sapiens ribosomal protein SA, mRNA (cDNA clone MGC:78527 IMAGE:5427114), complete cds. BC008867 - Homo sapiens ribosomal protein SA, mRNA (cDNA clone MGC:16750 IMAGE:4130936), complete cds. S37431 - 67 kda laminin receptor [human, lung cancer cell line SBC3, mRNA, 936 nt]. DQ891146 - Synthetic construct clone IMAGE:100003776; FLH169728.01X; RZPDo839F0696D ribosomal protein SA (RPSA) gene, encodes complete protein. DQ894326 - Synthetic construct Homo sapiens clone IMAGE:100008786; FLH169724.01L; RZPDo839F0695D ribosomal protein SA (RPSA) gene, encodes complete protein. KJ900699 - Synthetic construct Homo sapiens clone ccsbBroadEn_10093 RPSAP58 gene, encodes complete protein. CU679157 - Synthetic construct Homo sapiens gateway clone IMAGE:100016992 5' read RPSA mRNA. CU688210 - Synthetic construct Homo sapiens gateway clone IMAGE:100021130 5' read RPSA mRNA. AB464170 - Synthetic construct DNA, clone: pF1KB3446, Homo sapiens RPSA gene for ribosomal protein SA, without stop codon, in Flexi system. KJ905818 - Synthetic construct Homo sapiens clone ccsbBroadEn_15488 RPSA gene, encodes complete protein. BT007219 - Homo sapiens laminin receptor 1 (ribosomal protein SA, 67kDa) mRNA, complete cds. KJ891534 - Synthetic construct Homo sapiens clone ccsbBroadEn_00928 RPSA gene, encodes complete protein. KJ897119 - Synthetic construct Homo sapiens clone ccsbBroadEn_06513 RPSA gene, encodes complete protein. KJ897120 - Synthetic construct Homo sapiens clone ccsbBroadEn_06514 RPSA gene, encodes complete protein. JD024801 - Sequence 5825 from Patent EP1572962. JD021508 - Sequence 2532 from Patent EP1572962. JD292109 - Sequence 273133 from Patent EP1572962. JD268759 - Sequence 249783 from Patent EP1572962. JD268760 - Sequence 249784 from Patent EP1572962. X61156 - H.sapiens mRNA for laminin-binding protein. JD034630 - Sequence 15654 from Patent EP1572962. LF325263 - JP 2014500723-A/132766: Polycomb-Associated Non-Coding RNAs. MA560840 - JP 2018138019-A/132766: Polycomb-Associated Non-Coding RNAs. LF381796 - JP 2014500723-A/189299: Polycomb-Associated Non-Coding RNAs. MA617373 - JP 2018138019-A/189299: Polycomb-Associated Non-Coding RNAs. LF381808 - JP 2014500723-A/189311: Polycomb-Associated Non-Coding RNAs. MA617385 - JP 2018138019-A/189311: Polycomb-Associated Non-Coding RNAs. LQ990185 - Sequence 1 from Patent WO2013042053. DQ595111 - Homo sapiens piRNA piR-61223, complete sequence. AK055991 - Homo sapiens cDNA FLJ31429 fis, clone NT2NE2000536, moderately similar to 40S RIBOSOMAL PROTEIN SA. DQ585854 - Homo sapiens piRNA piR-52966, complete sequence. LF318786 - JP 2014500723-A/126289: Polycomb-Associated Non-Coding RNAs. MA554363 - JP 2018138019-A/126289: Polycomb-Associated Non-Coding RNAs. LF325261 - JP 2014500723-A/132764: Polycomb-Associated Non-Coding RNAs. MA560838 - JP 2018138019-A/132764: Polycomb-Associated Non-Coding RNAs. JD059231 - Sequence 40255 from Patent EP1572962. JD123924 - Sequence 104948 from Patent EP1572962. LF379431 - JP 2014500723-A/186934: Polycomb-Associated Non-Coding RNAs. MA615008 - JP 2018138019-A/186934: Polycomb-Associated Non-Coding RNAs. DQ592150 - Homo sapiens piRNA piR-59262, complete sequence. DQ585311 - Homo sapiens piRNA piR-52423, complete sequence. JD283515 - Sequence 264539 from Patent EP1572962. AF116719 - Homo sapiens PRO2987 mRNA, complete cds. M14199 - Human laminin receptor (2H5 epitope) mRNA, 5' end. LF318787 - JP 2014500723-A/126290: Polycomb-Associated Non-Coding RNAs. MA554364 - JP 2018138019-A/126290: Polycomb-Associated Non-Coding RNAs. JD133711 - Sequence 114735 from Patent EP1572962. JD044791 - Sequence 25815 from Patent EP1572962. LQ990186 - Sequence 2 from Patent WO2013042053. JD247592 - Sequence 228616 from Patent EP1572962. JD158024 - Sequence 139048 from Patent EP1572962. JD367850 - Sequence 348874 from Patent EP1572962. LF379435 - JP 2014500723-A/186938: Polycomb-Associated Non-Coding RNAs. MA615012 - JP 2018138019-A/186938: Polycomb-Associated Non-Coding RNAs. JD185082 - Sequence 166106 from Patent EP1572962. JD185083 - Sequence 166107 from Patent EP1572962. JD488452 - Sequence 469476 from Patent EP1572962. JD494328 - Sequence 475352 from Patent EP1572962.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_prionPathway - Prion Pathway
Reactome (by CSHL, EBI, and GO)
Protein P08865 (Reactome details) participates in the following event(s):
R-HSA-72676 eIF3 and eIF1A bind to the 40S subunit R-HSA-72673 Release of 40S and 60S subunits from the 80S ribosome R-HSA-72672 The 60S subunit joins the translation initiation complex R-HSA-72619 eIF2:GTP is hydrolyzed, eIFs are released R-HSA-72691 Formation of the 43S pre-initiation complex R-HSA-156808 Formation of translation initiation complexes yielding circularized Ceruloplasmin mRNA in a 'closed-loop' conformation R-HSA-157849 Formation of translation initiation complexes containing mRNA that does not circularize R-HSA-72671 eIF5B:GTP is hydrolyzed and released R-HSA-156907 Aminoacyl-tRNA binds to the ribosome at the A-site R-HSA-2408529 Sec-tRNA(Sec):EEFSEC:GTP binds to 80S Ribosome R-HSA-141691 GTP bound eRF3:eRF1 complex binds the peptidyl tRNA:mRNA:80S Ribosome complex R-HSA-156915 Translocation of ribosome by 3 bases in the 3' direction R-HSA-141671 Polypeptide release from the eRF3-GDP:eRF1:mRNA:80S Ribosome complex R-HSA-156912 Peptide transfer from P-site tRNA to the A-site tRNA R-HSA-927832 UPF1 binds an mRNP with a termination codon preceding an Exon Junction Complex R-HSA-927789 Formation of UPF1:eRF3 complex on mRNA with a premature termination codon and no Exon Junction Complex R-HSA-1799332 Nascent polypeptide:mRNA:ribosome complex binds signal recognition particle (SRP) R-HSA-156923 Hydrolysis of eEF1A:GTP R-HSA-72621 Ribosomal scanning R-HSA-72697 Start codon recognition R-HSA-156823 Association of phospho-L13a with GAIT element of Ceruloplasmin mRNA R-HSA-5333615 80S:Met-tRNAi:mRNA:SECISBP2:Sec-tRNA(Sec):EEFSEC:GTP is hydrolysed to 80S:Met-tRNAi:mRNA:SECISBP2:Sec and EEFSEC:GDP by EEFSEC R-HSA-141673 GTP Hydrolysis by eRF3 bound to the eRF1:mRNA:polypeptide:80S Ribosome complex R-HSA-927889 SMG1 phosphorylates UPF1 (enhanced by Exon Junction Complex) R-HSA-1799329 Signal peptidase hydrolyzes signal peptide from ribosome-associated nascent protein R-HSA-1799330 The SRP receptor binds the SRP:nascent peptide:ribosome complex R-HSA-1799326 Signal-containing nascent peptide translocates to endoplasmic reticulum R-HSA-72689 Formation of a pool of free 40S subunits R-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol R-HSA-1799339 SRP-dependent cotranslational protein targeting to membrane R-HSA-9010553 Regulation of expression of SLITs and ROBOs R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit R-HSA-72695 Formation of the ternary complex, and subsequently, the 43S complex R-HSA-72649 Translation initiation complex formation R-HSA-72737 Cap-dependent Translation Initiation R-HSA-8868773 rRNA processing in the nucleus and cytosol R-HSA-156902 Peptide chain elongation R-HSA-2408557 Selenocysteine synthesis R-HSA-72764 Eukaryotic Translation Termination R-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) R-HSA-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-HSA-72766 Translation R-HSA-376176 Signaling by ROBO receptors R-HSA-192823 Viral mRNA Translation R-HSA-72702 Ribosomal scanning and start codon recognition R-HSA-156827 L13a-mediated translation R-HSA-72662 Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S R-HSA-72613 Eukaryotic Translation Initiation R-HSA-72312 rRNA processing R-HSA-156842 Eukaryotic Translation Elongation R-HSA-2408522 Selenoamino acid metabolism R-HSA-927802 Nonsense-Mediated Decay (NMD) R-HSA-392499 Metabolism of proteins R-HSA-422475 Axon guidance R-HSA-168273 Influenza Viral RNA Transcription and Replication R-HSA-8953854 Metabolism of RNA R-HSA-71291 Metabolism of nitrogenous molecules R-HSA-1266738 Developmental Biology R-HSA-168255 Influenza Life Cycle R-HSA-1430728 Metabolism R-HSA-168254 Influenza Infection R-HSA-5663205 Infectious disease R-HSA-1643685 Disease
US Server
