Human Gene MRPS28 (ENST00000520946.1) from GENCODE V44
  Description: The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data. (from UniProt E5RK86)
RefSeq Summary (NM_014018): Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has been called mitochondrial ribosomal protein S35 in the literature. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000520946.1
Gencode Gene: ENSG00000147586.10
Transcript (Including UTRs)
   Position: hg38 chr8:79,919,032-80,029,974 Size: 110,943 Total Exon Count: 4 Strand: -
Coding Region
   Position: hg38 chr8:79,919,032-80,028,781 Size: 109,750 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesmRNA DescriptionsOther Names
Methods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr8:79,919,032-80,029,974)mRNA (may differ from genome)Protein (108 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblExonPrimerGencodeGeneCardsHGNC
LynxMalacardsMGIPubMedUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: E5RK86_HUMAN
DESCRIPTION: SubName: Full=28S ribosomal protein S28, mitochondrial; Flags: Fragment;
CAUTION: The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 28.73 RPKM in Adrenal Gland
Total median expression: 578.45 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -32.3085-0.380 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR019375 - Ribosomal_S28_mit
IPR022967 - RNA-binding_domain_S1

Pfam Domains:
PF10246 - Mitochondrial ribosomal protein MRP-S35

ModBase Predicted Comparative 3D Structure on E5RK86
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Descriptions from all associated GenBank mRNAs
  AF070663 - Homo sapiens HSPC007 mRNA, complete cds.
AK026506 - Homo sapiens cDNA: FLJ22853 fis, clone KAT00869, highly similar to AF070663 Homo sapiens HSPC007 mRNA.
AK223069 - Homo sapiens mRNA for mitochondrial ribosomal protein S28 variant, clone: KAT00869.
BC010150 - Homo sapiens mitochondrial ribosomal protein S28, mRNA (cDNA clone MGC:19500 IMAGE:4331173), complete cds.
EF152584 - Homo sapiens PC1/MRPS28 fusion protein mRNA, complete cds.
AK315739 - Homo sapiens cDNA, FLJ96848, Homo sapiens mitochondrial ribosomal protein S28 (MRPS28), nucleargene encoding mitochondrial protein, mRNA.
JF432581 - Synthetic construct Homo sapiens clone IMAGE:100073817 mitochondrial ribosomal protein S28 (MRPS28) gene, encodes complete protein.
KU178612 - Homo sapiens mitochondrial ribosomal protein S28 isoform 1 (MRPS28) mRNA, partial cds; nuclear gene for mitochondrial product.
KU178613 - Homo sapiens mitochondrial ribosomal protein S28 isoform 2 (MRPS28) mRNA, partial cds, alternatively spliced; nuclear gene for mitochondrial product.
KU178614 - Homo sapiens mitochondrial ribosomal protein S28 isoform 4 (MRPS28) mRNA, partial cds, alternatively spliced; nuclear gene for mitochondrial product.

-  Other Names for This Gene
  Alternate Gene Symbols: E5RK86, E5RK86_HUMAN, KU178614, uc064nwx.1
UCSC ID: ENST00000520946.1
RefSeq Accession: NM_014018
Protein: E5RK86

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.