Description: RNA polymerase III transcription initiation RefSeq Summary (NM_018310): This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. [provided by RefSeq, Jul 2008]. Transcript (Including UTRs) Position: hg18 chr8:37,820,561-37,826,569 Size: 6,009 Total Exon Count: 4 Strand: - Coding Region Position: hg18 chr8:37,821,166-37,826,459 Size: 5,294 Coding Exon Count: 4
ID:BRF2_HUMAN DESCRIPTION: RecName: Full=Transcription factor IIIB 50 kDa subunit; Short=TFIIIB50; Short=hTFIIIB50; AltName: Full=B-related factor 2; Short=BRF-2; AltName: Full=hBRFU; FUNCTION: General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. COFACTOR: Binds 1 zinc ion per subunit (By similarity). SUBUNIT: Component of TFIIIB complex. The TFIIIB complex has two activities, alpha and beta. The TFIIIB-alpha activity complex is composed of TBP, BDP1, and a complex containing both BRF2 and at least four stably associated proteins; this complex inhibits the transcription by pol III via its phosphorylation by CK2; YY1 facilitates the TFIIIB-alpha complex formation. BRF2 recruitment to the TATA box containing small nuclear RNA (snRNA) gene templates is TBP-dependent. Interacts with TBP; this interaction with TBP mediates its TATA-box recruitment of these promoters. Interacts with TBP and the BURE sequence (GC-rich sequence downstream from the TATA box) to form a strong ternary complex which is joined by BDP1; this ternary complex stimulates pol III transcription. Forms a trimeric complex composed of TBP, BRF2 and mini-SNAPc complex (SNAP43, SNAP50, and the N-terminal third of SNAP190) on the promoter. Assembly of the TBP-BRF2 complex is stimulated by SNAP190. Interacts with MAF1 and SNAPC4. SUBCELLULAR LOCATION: Nucleus. INDUCTION: Down-regulated by epigallocatechin gallate (EGCG) treatment. SIMILARITY: Belongs to the TFIIB family. SIMILARITY: Contains 1 TFIIB-type zinc finger. SEQUENCE CAUTION: Sequence=AAG35486.1; Type=Erroneous initiation;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9HAW0
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AF130058 - Homo sapiens clone FLB5619 PRO1470 mRNA, complete cds. BC010648 - Homo sapiens BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like, mRNA (cDNA clone MGC:9916 IMAGE:3871326), complete cds. AF206673 - Homo sapiens TFIIIB50 mRNA, complete cds. AK001914 - Homo sapiens cDNA FLJ11052 fis, clone PLACE1004645. AF298153 - Homo sapiens RNA polymerase III transcription initiation factor BRFU mRNA, complete cds. AK294337 - Homo sapiens cDNA FLJ51823 complete cds, highly similar to Homo sapiens BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (BRF2), mRNA. AK315420 - Homo sapiens cDNA, FLJ96474, Homo sapiens BRF2, subunit of RNA polymerase III transcriptioninitiation factor, BRF1-like (BRF2), mRNA. EU446514 - Synthetic construct Homo sapiens clone IMAGE:100069872; IMAGE:100011723; FLH258608.01L BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (BRF2) gene, encodes complete protein. AB463449 - Synthetic construct DNA, clone: pF1KB7707, Homo sapiens BRF2 gene for BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like, without stop codon, in Flexi system. CU679783 - Synthetic construct Homo sapiens gateway clone IMAGE:100017226 5' read BRF2 mRNA. JD448036 - Sequence 429060 from Patent EP1572962. JD450442 - Sequence 431466 from Patent EP1572962. JD547205 - Sequence 528229 from Patent EP1572962. KJ894179 - Synthetic construct Homo sapiens clone ccsbBroadEn_03573 BRF2 gene, encodes complete protein. JD021198 - Sequence 2222 from Patent EP1572962. JD033972 - Sequence 14996 from Patent EP1572962. JD243962 - Sequence 224986 from Patent EP1572962. JD226354 - Sequence 207378 from Patent EP1572962. JD178252 - Sequence 159276 from Patent EP1572962. JD255036 - Sequence 236060 from Patent EP1572962. JD060226 - Sequence 41250 from Patent EP1572962. JD162178 - Sequence 143202 from Patent EP1572962. JD043902 - Sequence 24926 from Patent EP1572962. JD053631 - Sequence 34655 from Patent EP1572962. JD234433 - Sequence 215457 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9HAW0 (Reactome details) participates in the following event(s):
R-HSA-83793 Binding of TFIIIB to SNAPc:Oct-1:Staf:Type 3 Promoter Complex R-HSA-83803 Recruitment of RNA Polymerase III to TFIIIB:SNAPc:Type 3 Promoter Complex R-HSA-112054 RNA Polymerase III Abortive Initiation At Type 3 Open Promoters R-HSA-112156 RNA Polymerase III Simple Start Sequence Initiation At Type 3 Promoters R-HSA-112152 RNA Polymerase III Promoter Opening at Type 3 Promoters R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation R-HSA-76046 RNA Polymerase III Transcription Initiation R-HSA-74158 RNA Polymerase III Transcription R-HSA-74160 Gene expression (Transcription)
US Server
