Human Gene NT5C1A (uc001cdq.1)
  Description: 5'-nucleotidase, cytosolic IA
RefSeq Summary (NM_032526): Cytosolic nucleotidases, such as NT5C1A, dephosphorylate nucleoside monophosphates (Hunsucker et al., 2001 [PubMed 11133996]).[supplied by OMIM, Mar 2008].
Transcript (Including UTRs)
   Position: hg18 chr1:39,897,380-39,910,297 Size: 12,918 Total Exon Count: 6 Strand: -
Coding Region
   Position: hg18 chr1:39,897,380-39,910,297 Size: 12,918 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDMicroarray Expression
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2009-03-03

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:39,897,380-39,910,297)mRNA (may differ from genome)Protein (368 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkHGNCHPRDMGIOMIMPubMed
ReactomeTreefamUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: 5NT1A_HUMAN
DESCRIPTION: RecName: Full=Cytosolic 5'-nucleotidase 1A; Short=cN1A; EC=3.1.3.5; AltName: Full=Cytosolic 5'-nucleotidase IA; Short=cN-I; Short=cN-IA;
FUNCTION: Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate adenosine levels in heart during ischemia and hypoxia.
CATALYTIC ACTIVITY: A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate.
COFACTOR: Magnesium.
ENZYME REGULATION: Activated by ADP.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in skeletal muscle. Detected at intermediate levels in heart, brain, kidney and pancreas.
SIMILARITY: Belongs to the 5'-nucleotidase type 3 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  Microarray Expression Data
 
Expression ratio colors:

GNF Expression Atlas 2 Data from U133A and GNF1H Chips

      
      
      
     
    
     
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Affymetrix All Exon Microarrays

           
Ratios

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR010394 - 5-nucleotidase

Pfam Domains:
PF06189 - 5'-nucleotidase

ModBase Predicted Comparative 3D Structure on Q9BXI3
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserNo orthologGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGI Ensembl   
Protein Sequence Protein Sequence   
Alignment Alignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0000287 magnesium ion binding
GO:0005515 protein binding
GO:0008253 5'-nucleotidase activity
GO:0016787 hydrolase activity

Biological Process:
GO:0006195 purine nucleotide catabolic process
GO:0009116 nucleoside metabolic process
GO:0009117 nucleotide metabolic process
GO:0009128 purine nucleoside monophosphate catabolic process
GO:0016311 dephosphorylation
GO:0046085 adenosine metabolic process
GO:0046135 pyrimidine nucleoside catabolic process

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  AF331801 - Homo sapiens cytosolic nucleotidase I mRNA, complete cds.
AY028778 - Homo sapiens 5'-nucleotidase I mRNA, complete cds.
BC103879 - Homo sapiens 5'-nucleotidase, cytosolic IA, mRNA (cDNA clone MGC:119199 IMAGE:40004489), complete cds.
BC103880 - Homo sapiens 5'-nucleotidase, cytosolic IA, mRNA (cDNA clone MGC:119201 IMAGE:40004491), complete cds.
KJ899811 - Synthetic construct Homo sapiens clone ccsbBroadEn_09205 NT5C1A gene, encodes complete protein.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00230 - Purine metabolism
hsa00240 - Pyrimidine metabolism
hsa00760 - Nicotinate and nicotinamide metabolism
hsa01100 - Metabolic pathways

Reactome (by CSHL, EBI, and GO)

Protein Q9BXI3 (Reactome details) participates in the following event(s):

R-HSA-109380 (d)CMP, TMP, or (d)UMP + H2O => (deoxy)cytidine, thymidine, or (deoxy)uridine + orthophosphate (NT5C1A)
R-HSA-109387 (d)AMP, (d)GMP, or (d)IMP + H2O => (deoxy)adenosine, (deoxy)guanosine, or (deoxy)inosine + orthophosphate (NT5C1A)
R-HSA-73621 Pyrimidine catabolism
R-HSA-74259 Purine catabolism
R-HSA-8956319 Nucleobase catabolism
R-HSA-15869 Metabolism of nucleotides
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: 5NT1A_HUMAN, NM_032526, NP_115915, Q3SYB9, Q5TG98, Q9BWT8, Q9BXI3
UCSC ID: uc001cdq.1
RefSeq Accession: NM_032526
Protein: Q9BXI3 (aka 5NT1A_HUMAN or 5N1A_HUMAN)
CCDS: CCDS440.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_032526.1
exon count: 6CDS single in 3' UTR: no RNA size: 1107
ORF size: 1107CDS single in intron: no Alignment % ID: 99.91
txCdsPredict score: 2409.50frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.