Description: p47 protein isoform a RefSeq Summary (NM_016143): N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. [provided by RefSeq, May 2011]. Transcript (Including UTRs) Position: hg18 chr20:1,370,811-1,396,417 Size: 25,607 Total Exon Count: 9 Strand: - Coding Region Position: hg18 chr20:1,372,394-1,395,469 Size: 23,076 Coding Exon Count: 9
ID:NSF1C_HUMAN DESCRIPTION: RecName: Full=NSFL1 cofactor p47; AltName: Full=UBX domain-containing protein 2C; AltName: Full=p97 cofactor p47; FUNCTION: Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP- mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro). SUBUNIT: Part of a ternary complex containing STX5A, NSFL1C and VCP. NSFL1C forms a homotrimer that binds to one end of a VCP homohexamer. The complex binds to membranes enriched in phosphatidylethanolamine-containing lipids and promotes Golgi membrane fusion. Interaction with VCIP135 leads to dissociation of the complex via ATP hydrolysis by VCP. Binds ubiquitin and mono- ubiquitinated proteins via its N-terminal UBA-like domain when bound to VCP (By similarity). SUBCELLULAR LOCATION: Nucleus (By similarity). Golgi apparatus, Golgi stack (By similarity). Chromosome (By similarity). Note=Predominantly nuclear in interphase cells. Bound to the axial elements of sex chromosomes in pachytene spermatocytes. A small proportion of the protein is cytoplasmic, associated with Golgi stacks (By similarity). PTM: Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis (By similarity). SIMILARITY: Belongs to the NSFL1C family. SIMILARITY: Contains 1 SEP domain. SIMILARITY: Contains 1 UBX domain. SEQUENCE CAUTION: Sequence=AAF17199.1; Type=Frameshift; Positions=Several; Sequence=AAF17199.1; Type=Miscellaneous discrepancy; Note=Sequencing errors;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9UNZ2
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
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Sequence and Links to Tools and Databases 
