Human Gene PI4K2A (uc001kog.1)
  Description: phosphatidylinositol 4-kinase type 2 alpha
RefSeq Summary (NM_018425): Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 [PubMed 11244087]).[supplied by OMIM, Mar 2008].
Transcript (Including UTRs)
   Position: hg18 chr10:99,390,433-99,426,177 Size: 35,745 Total Exon Count: 9 Strand: +
Coding Region
   Position: hg18 chr10:99,390,490-99,423,489 Size: 33,000 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathways
Other NamesModel InformationMethods
Data last updated at UCSC: 2009-03-03

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr10:99,390,433-99,426,177)mRNA (may differ from genome)Protein (479 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
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PubMedReactomeTreefamUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: P4K2A_HUMAN
DESCRIPTION: RecName: Full=Phosphatidylinositol 4-kinase type 2-alpha; EC=2.7.1.67; AltName: Full=Phosphatidylinositol 4-kinase type II-alpha;
FUNCTION: Together with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells (By similarity).
CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.
SUBUNIT: Associates with the BLOC-1 and the AP-3 complexes; the BLOC-1 complex is required for optimal binding of PI4K2A to the AP-3 complex. Interacts with BLOC1S5 and DTNBP1.
SUBCELLULAR LOCATION: Cytoplasm. Membrane (By similarity). Membrane raft. Cell projection, dendrite (By similarity). Cell junction, synapse, presynaptic cell membrane (By similarity). Cell junction, synapse, synaptosome (By similarity). Mitochondrion (By similarity). Endosome (By similarity). Cytoplasmic vesicle (By similarity). Note=Localized in neuronal cell body. Transported from neuronal cell body to neuron projections in a BLOC-1- and AP- 3-complexes-dependent manner. Enriched in neurite tips and neuron projections in a BLOC-1- and AP-3-complexes-dependent manner (By similarity). Found in subdomains of the plasma membrane termed non-caveolar membrane rafts.
TISSUE SPECIFICITY: Widely expressed. Highest expression is observed in kidney, brain, heart, skeletal muscle, and placenta and lowest expression is observed in colon, thymus, and small intestine.
SIMILARITY: Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.
SIMILARITY: Contains 1 PI3K/PI4K domain.

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

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Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  Microarray Expression Data
 
Expression ratio colors:

GNF Expression Atlas 2 Data from U133A and GNF1H Chips

      
      
      
     
    
     
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Affymetrix All Exon Microarrays

           
Ratios

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -21.8057-0.382 Picture PostScript Text
3' UTR -993.662688-0.370 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000403 - PI3/4_kinase_cat_dom

Pfam Domains:
PF00454 - Phosphatidylinositol 3- and 4-kinase

SCOP Domains:
56399 - ADP-ribosylation

ModBase Predicted Comparative 3D Structure on Q9BTU6
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserNo orthologGenome BrowserGenome BrowserNo orthologGenome Browser
Gene DetailsGene Details Gene Details Gene Details
Gene SorterGene Sorter Gene Sorter Gene Sorter
MGIRGDEnsemblFlyBase SGD
Protein Sequence Protein SequenceProtein Sequence Protein Sequence
Alignment AlignmentAlignment Alignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0000287 magnesium ion binding
GO:0004430 1-phosphatidylinositol 4-kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0035651 AP-3 adaptor complex binding
GO:0044877 macromolecular complex binding

Biological Process:
GO:0002561 basophil degranulation
GO:0006661 phosphatidylinositol biosynthetic process
GO:0007030 Golgi organization
GO:0007032 endosome organization
GO:0016310 phosphorylation
GO:0046854 phosphatidylinositol phosphorylation

Cellular Component:
GO:0005739 mitochondrion
GO:0005765 lysosomal membrane
GO:0005768 endosome
GO:0005794 Golgi apparatus
GO:0005802 trans-Golgi network
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0030054 cell junction
GO:0030425 dendrite
GO:0030672 synaptic vesicle membrane
GO:0031224 intrinsic component of membrane
GO:0031410 cytoplasmic vesicle
GO:0031901 early endosome membrane
GO:0032991 macromolecular complex
GO:0035838 growing cell tip
GO:0042734 presynaptic membrane
GO:0042995 cell projection
GO:0043005 neuron projection
GO:0043025 neuronal cell body
GO:0043204 perikaryon
GO:0043231 intracellular membrane-bounded organelle
GO:0044231 host cell presynaptic membrane
GO:0045121 membrane raft
GO:0045202 synapse
GO:0070382 exocytic vesicle
GO:0031083 BLOC-1 complex


-  Descriptions from all associated GenBank mRNAs
  AK293733 - Homo sapiens cDNA FLJ56960 complete cds, highly similar to Homo sapiens phosphatidylinositol 4-kinase type II (PI4KII), mRNA.
AJ303098 - Homo sapiens mRNA for type II alpha phosphatidylinositol 4-kinase gene.
BC003167 - Homo sapiens phosphatidylinositol 4-kinase type 2 alpha, mRNA (cDNA clone MGC:4395 IMAGE:2905670), complete cds.
DQ893758 - Synthetic construct Homo sapiens clone IMAGE:100008218; FLH164270.01L; RZPDo839E07161D phosphatidylinositol 4-kinase type II (PI4KII) gene, encodes complete protein.
DQ893537 - Synthetic construct clone IMAGE:100006167; FLH164274.01X; RZPDo839E07162D phosphatidylinositol 4-kinase type II (PI4KII) gene, encodes complete protein.
BT007330 - Homo sapiens phosphatidylinositol 4-kinase type II mRNA, complete cds.
AK309343 - Homo sapiens cDNA, FLJ99384.
AL353952 - Homo sapiens mRNA; cDNA DKFZp761G1923 (from clone DKFZp761G1923).
AK024317 - Homo sapiens cDNA FLJ14255 fis, clone OVARC1001873.
AK023236 - Homo sapiens cDNA FLJ13174 fis, clone NT2RP3003833.
AF070611 - Homo sapiens clones 24718 and 24825 mRNA sequence.
DL492214 - Novel nucleic acids.
JD516897 - Sequence 497921 from Patent EP1572962.
JD358465 - Sequence 339489 from Patent EP1572962.
JD449046 - Sequence 430070 from Patent EP1572962.
JD490812 - Sequence 471836 from Patent EP1572962.
JD487945 - Sequence 468969 from Patent EP1572962.
JD516613 - Sequence 497637 from Patent EP1572962.
JD567079 - Sequence 548103 from Patent EP1572962.
JD493810 - Sequence 474834 from Patent EP1572962.
JD368805 - Sequence 349829 from Patent EP1572962.
JD382805 - Sequence 363829 from Patent EP1572962.
JD554118 - Sequence 535142 from Patent EP1572962.
JD420056 - Sequence 401080 from Patent EP1572962.
JD500751 - Sequence 481775 from Patent EP1572962.
JD419117 - Sequence 400141 from Patent EP1572962.
JD494755 - Sequence 475779 from Patent EP1572962.
JD407825 - Sequence 388849 from Patent EP1572962.
JD442389 - Sequence 423413 from Patent EP1572962.
JD465968 - Sequence 446992 from Patent EP1572962.
JD430800 - Sequence 411824 from Patent EP1572962.
JD407838 - Sequence 388862 from Patent EP1572962.
JD399727 - Sequence 380751 from Patent EP1572962.
JD434336 - Sequence 415360 from Patent EP1572962.
JD325347 - Sequence 306371 from Patent EP1572962.
JD046992 - Sequence 28016 from Patent EP1572962.
JD092946 - Sequence 73970 from Patent EP1572962.
JD177729 - Sequence 158753 from Patent EP1572962.
JD336198 - Sequence 317222 from Patent EP1572962.
JD116024 - Sequence 97048 from Patent EP1572962.
JD271458 - Sequence 252482 from Patent EP1572962.
JD202049 - Sequence 183073 from Patent EP1572962.
JD108400 - Sequence 89424 from Patent EP1572962.
JD177576 - Sequence 158600 from Patent EP1572962.
JD300752 - Sequence 281776 from Patent EP1572962.
JD188784 - Sequence 169808 from Patent EP1572962.
JD051890 - Sequence 32914 from Patent EP1572962.
JD306867 - Sequence 287891 from Patent EP1572962.
JD065741 - Sequence 46765 from Patent EP1572962.
JD109738 - Sequence 90762 from Patent EP1572962.
JD163443 - Sequence 144467 from Patent EP1572962.
JD119845 - Sequence 100869 from Patent EP1572962.
JD167944 - Sequence 148968 from Patent EP1572962.
JD201602 - Sequence 182626 from Patent EP1572962.
JD333470 - Sequence 314494 from Patent EP1572962.
JD047152 - Sequence 28176 from Patent EP1572962.
JD334586 - Sequence 315610 from Patent EP1572962.
JD099866 - Sequence 80890 from Patent EP1572962.
JD189158 - Sequence 170182 from Patent EP1572962.
JD267442 - Sequence 248466 from Patent EP1572962.
JD248008 - Sequence 229032 from Patent EP1572962.
JD297936 - Sequence 278960 from Patent EP1572962.
JD255594 - Sequence 236618 from Patent EP1572962.
JD056449 - Sequence 37473 from Patent EP1572962.
JD156923 - Sequence 137947 from Patent EP1572962.
JD270764 - Sequence 251788 from Patent EP1572962.
JD136952 - Sequence 117976 from Patent EP1572962.
LF385291 - JP 2014500723-A/192794: Polycomb-Associated Non-Coding RNAs.
LF345638 - JP 2014500723-A/153141: Polycomb-Associated Non-Coding RNAs.
LF345639 - JP 2014500723-A/153142: Polycomb-Associated Non-Coding RNAs.
MA620868 - JP 2018138019-A/192794: Polycomb-Associated Non-Coding RNAs.
MA581215 - JP 2018138019-A/153141: Polycomb-Associated Non-Coding RNAs.
MA581216 - JP 2018138019-A/153142: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00562 - Inositol phosphate metabolism
hsa01100 - Metabolic pathways
hsa04070 - Phosphatidylinositol signaling system

Reactome (by CSHL, EBI, and GO)

Protein Q9BTU6 (Reactome details) participates in the following event(s):

R-HSA-1675780 PI is phosphorylated to PI4P by PI4K2A/B at the plasma membrane
R-HSA-1675974 PI is phosphorylated to PI4P by PI4K2A/B at the early endosome membrane
R-HSA-1676185 PI is phosphorylated to PI4P by PI4KA/2A/2B at the Golgi membrane
R-HSA-1660499 Synthesis of PIPs at the plasma membrane
R-HSA-1660516 Synthesis of PIPs at the early endosome membrane
R-HSA-1660514 Synthesis of PIPs at the Golgi membrane
R-HSA-1483255 PI Metabolism
R-HSA-1483257 Phospholipid metabolism
R-HSA-556833 Metabolism of lipids
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: NM_018425, NP_060895, P4K2A_HUMAN, Q9BTU6, Q9NSG8
UCSC ID: uc001kog.1
RefSeq Accession: NM_018425
Protein: Q9BTU6 (aka P4K2A_HUMAN)
CCDS: CCDS7469.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_018425.2
exon count: 9CDS single in 3' UTR: no RNA size: 4185
ORF size: 1440CDS single in intron: no Alignment % ID: 99.98
txCdsPredict score: 2879.50frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.