Human Gene NOS1AP (uc009wut.1)
  Description: nitric oxide synthase 1 (neuronal) adaptor
RefSeq Summary (NM_001126060): This gene encodes a cytosolic protein that binds to the signaling molecule, neuronal nitric oxide synthase (nNOS). This protein has a C-terminal PDZ-binding domain that mediates interactions with nNOS and an N-terminal phosphotyrosine binding (PTB) domain that binds to the small monomeric G protein, Dexras1. Studies of the related mouse and rat proteins have shown that this protein functions as an adapter protein linking nNOS to specific targets, such as Dexras1 and the synapsins. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2009].
Transcript (Including UTRs)
   Position: hg18 chr1:160,599,397-160,606,437 Size: 7,041 Total Exon Count: 2 Strand: +
Coding Region
   Position: hg18 chr1:160,599,737-160,599,886 Size: 150 Coding Exon Count: 1 

Page IndexSequence and LinksPrimersGenetic AssociationsCTDMicroarray Expression
RNA StructureOther SpeciesmRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2009-03-03

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:160,599,397-160,606,437)mRNA (may differ from genome)Protein (49 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
EnsemblEntrez GeneExonPrimerGeneCardsGeneNetworkH-INV
HGNCHuGEMGIOMIMPubMedTreefam
BioGrid CRISPR DB

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): NOS1AP
CDC HuGE Published Literature: NOS1AP
Positive Disease Associations: cardiac death QT interval
Related Studies:
  1. cardiac death QT interval
    Aarnoudse, A. J. et al. 2007, Common NOS1AP Variants Are Associated With a Prolonged QTc Interval in the Rotterdam Study, Circulation 2007. [PubMed 17576865]
    Common variants in NOS1AP are strongly associated with QT-interval duration in an elderly population. Larger sample sizes are needed to confirm or exclude an effect on sudden cardiac death risk.

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  Microarray Expression Data
 
Expression ratio colors:

GNF Expression Atlas 2 Data from U133A and GNF1H Chips

      
      
      
     
    
     
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Affymetrix All Exon Microarrays

           
Ratios

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -79.90340-0.235 Picture PostScript Text
3' UTR -1855.415069-0.366 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Descriptions from all associated GenBank mRNAs
  BC041382 - Homo sapiens nitric oxide synthase 1 (neuronal) adaptor protein, mRNA (cDNA clone IMAGE:5275285).
AB007933 - Homo sapiens KIAA0464 mRNA for KIAA0464 protein.
BC112295 - Homo sapiens nitric oxide synthase 1 (neuronal) adaptor protein, mRNA (cDNA clone MGC:138500 IMAGE:8327763), complete cds.
BC143771 - Homo sapiens cDNA clone MGC:177302 IMAGE:9052285, complete cds.
BC143780 - Homo sapiens cDNA clone IMAGE:9052294.
BC111928 - Synthetic construct Homo sapiens clone IMAGE:40080755, MGC:133468 NOS1AP protein (NOS1AP) mRNA, encodes complete protein.
BC118499 - Synthetic construct Homo sapiens clone IMAGE:40080763, MGC:155336 NOS1AP protein (NOS1AP) mRNA, encodes complete protein.
AB587549 - Synthetic construct DNA, clone: pF1KE1067, Homo sapiens NOS1AP gene for nitric oxide synthase 1 (neuronal) adaptor protein, without stop codon, in Flexi system.
AY841899 - Homo sapiens CAPON short form mRNA, complete cds, alternatively spliced.
AF037070 - Homo sapiens carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase mRNA, partial cds.
JD424606 - Sequence 405630 from Patent EP1572962.
JD488227 - Sequence 469251 from Patent EP1572962.
JD390195 - Sequence 371219 from Patent EP1572962.
JD384187 - Sequence 365211 from Patent EP1572962.
JD443118 - Sequence 424142 from Patent EP1572962.
JD465882 - Sequence 446906 from Patent EP1572962.
JD420963 - Sequence 401987 from Patent EP1572962.
JD484046 - Sequence 465070 from Patent EP1572962.
JD138726 - Sequence 119750 from Patent EP1572962.
JD228748 - Sequence 209772 from Patent EP1572962.
JD158090 - Sequence 139114 from Patent EP1572962.
JD232891 - Sequence 213915 from Patent EP1572962.
JD300540 - Sequence 281564 from Patent EP1572962.
JD255154 - Sequence 236178 from Patent EP1572962.
JD323232 - Sequence 304256 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: NM_001126060, NP_001119532, uc001gbv.1
UCSC ID: uc009wut.1
RefSeq Accession: NM_001126060

-  Gene Model Information
 
category: coding nonsense-mediated-decay: yes RNA accession: NM_001126060.1
exon count: 2CDS single in 3' UTR: no RNA size: 5559
ORF size: 150CDS single in intron: yes Alignment % ID: 100.00
txCdsPredict score: -43.50frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 2421# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.