Human Gene MRP63 (uc001unw.1)
  Description: mitochondrial ribosomal protein 63
RefSeq Summary (NM_024026): Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein which belongs to an undetermined ribosomal subunit and which seems to be specific to animal mitoribosomes. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 3p, 5q, 8q, 14q, and Y. [provided by RefSeq, Jul 2008].
Transcript (Including UTRs)
   Position: hg18 chr13:20,648,372-20,651,220 Size: 2,849 Total Exon Count: 2 Strand: +
Coding Region
   Position: hg18 chr13:20,649,056-20,649,364 Size: 309 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathways
Other NamesModel InformationMethods
Data last updated at UCSC: 2009-03-03

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr13:20,648,372-20,651,220)mRNA (may differ from genome)Protein (102 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsGeneNetwork
H-INVHGNCHPRDMGIOMIMPubMed
ReactomeTreefamUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: RT63_HUMAN
DESCRIPTION: RecName: Full=Ribosomal protein 63, mitochondrial; Short=hMRP63;
SUBCELLULAR LOCATION: Mitochondrion (By similarity).
MISCELLANEOUS: Identified only in the intact 55S subunit. It is unknown whether it belongs to the 28S or to the 39S subunit. May localize at the subunit interface and dissociate from the 55S mitoribosome during subunit separation.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  Microarray Expression Data
 
Expression ratio colors:

GNF Expression Atlas 2 Data from U133A and GNF1H Chips

      
      
      
     
    
     
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Affymetrix All Exon Microarrays

           
Ratios

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -212.70490-0.434 Picture PostScript Text
3' UTR -591.111856-0.318 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR016576 - Ribosomal_MRP63_mit

ModBase Predicted Comparative 3D Structure on Q9BQC6
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGI     
Protein Sequence     
Alignment     

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003735 structural constituent of ribosome

Biological Process:
GO:0006412 translation
GO:0032543 mitochondrial translation
GO:0070125 mitochondrial translational elongation
GO:0070126 mitochondrial translational termination

Cellular Component:
GO:0005739 mitochondrion
GO:0005743 mitochondrial inner membrane
GO:0005761 mitochondrial ribosome
GO:0005840 ribosome


-  Descriptions from all associated GenBank mRNAs
  BC068492 - Homo sapiens mitochondrial ribosomal protein 63, mRNA (cDNA clone MGC:87182 IMAGE:4801279), complete cds.
BC023616 - Homo sapiens mitochondrial ribosomal protein 63, mRNA (cDNA clone MGC:23340 IMAGE:4645641), complete cds.
BC000002 - Homo sapiens mitochondrial ribosomal protein 63, mRNA (cDNA clone IMAGE:3505092), with apparent retained intron.
AB049957 - Homo sapiens bMRP63 mRNA for mitochondrial ribosomal protein bMRP63, complete cds.
DQ895281 - Synthetic construct Homo sapiens clone IMAGE:100009741; FLH184620.01L; RZPDo839A02145D mitochondrial ribosomal protein 63 (MRP63) gene, encodes complete protein; nuclear gene for mitochondrial product.
DQ892088 - Synthetic construct clone IMAGE:100004718; FLH184624.01X; RZPDo839A02146D mitochondrial ribosomal protein 63 (MRP63) gene, encodes complete protein; nuclear gene for mitochondrial product.
JD446612 - Sequence 427636 from Patent EP1572962.
JD362327 - Sequence 343351 from Patent EP1572962.
JD489808 - Sequence 470832 from Patent EP1572962.
JD447072 - Sequence 428096 from Patent EP1572962.
JD468877 - Sequence 449901 from Patent EP1572962.
JD453175 - Sequence 434199 from Patent EP1572962.
JD431427 - Sequence 412451 from Patent EP1572962.
JD529589 - Sequence 510613 from Patent EP1572962.
JD501338 - Sequence 482362 from Patent EP1572962.
JD534108 - Sequence 515132 from Patent EP1572962.
JD442277 - Sequence 423301 from Patent EP1572962.
JD354273 - Sequence 335297 from Patent EP1572962.
KJ899506 - Synthetic construct Homo sapiens clone ccsbBroadEn_08900 MRP63 gene, encodes complete protein.
JD321538 - Sequence 302562 from Patent EP1572962.
JD180693 - Sequence 161717 from Patent EP1572962.
JD338479 - Sequence 319503 from Patent EP1572962.
JD225474 - Sequence 206498 from Patent EP1572962.
JD262929 - Sequence 243953 from Patent EP1572962.
JD285967 - Sequence 266991 from Patent EP1572962.
JD101721 - Sequence 82745 from Patent EP1572962.
JD319777 - Sequence 300801 from Patent EP1572962.
JD244278 - Sequence 225302 from Patent EP1572962.
JD108197 - Sequence 89221 from Patent EP1572962.
JD047718 - Sequence 28742 from Patent EP1572962.
JD266491 - Sequence 247515 from Patent EP1572962.
JD344071 - Sequence 325095 from Patent EP1572962.
JD178667 - Sequence 159691 from Patent EP1572962.
JD133667 - Sequence 114691 from Patent EP1572962.
JD170017 - Sequence 151041 from Patent EP1572962.
JD306758 - Sequence 287782 from Patent EP1572962.
JD292295 - Sequence 273319 from Patent EP1572962.
JD138095 - Sequence 119119 from Patent EP1572962.
JD064930 - Sequence 45954 from Patent EP1572962.
JD177335 - Sequence 158359 from Patent EP1572962.
JD271329 - Sequence 252353 from Patent EP1572962.
JD231942 - Sequence 212966 from Patent EP1572962.
JD283400 - Sequence 264424 from Patent EP1572962.
JD199370 - Sequence 180394 from Patent EP1572962.
MN309624 - Homo sapiens LNC_000987 lncRNA, complete sequence.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9BQC6 (Reactome details) participates in the following event(s):

R-HSA-5389839 39S subunit binds 28S subunit:mRNA:fMet-tRNA
R-HSA-5419273 Hydrolysis of GTP and dissociation of 28S and 39S subunits
R-HSA-5389848 TUFM:GTP:aminoacyl-tRNA binds 55S ribosome:mRNA:fMet-tRNA
R-HSA-5389857 Peptide transfer from P-site to A-site (peptide bond formation)
R-HSA-5389842 TUFM hydrolyzes GTP and TUFM:GDP dissociates from 55S ribosome
R-HSA-5419261 55S ribosome with peptidyl-tRNA in A site binds GFM1:GTP
R-HSA-5419281 MRRF binds 55S ribosome:mRNA:tRNA
R-HSA-5419279 Translocation of peptidyl-tRNA from A-site to P-site (and translocation of 55S ribosome by 3 bases along mRNA)
R-HSA-5419264 MTRF1L (MTRF1a) or ICT1 binds stop codon in 55S ribosome:mRNA:peptidyl-tRNA
R-HSA-5419277 GFM2:GTP binds 55S ribosome:mRNA:tRNA:MRRF releasing mRNA and tRNA
R-HSA-5368286 Mitochondrial translation initiation
R-HSA-5419276 Mitochondrial translation termination
R-HSA-5368287 Mitochondrial translation
R-HSA-5389840 Mitochondrial translation elongation
R-HSA-72766 Translation
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: NM_024026, NP_076931, Q9BQC6, RT63_HUMAN
UCSC ID: uc001unw.1
RefSeq Accession: NM_024026
Protein: Q9BQC6 (aka RT63_HUMAN)
CCDS: CCDS9296.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_024026.4
exon count: 2CDS single in 3' UTR: no RNA size: 2671
ORF size: 309CDS single in intron: no Alignment % ID: 99.96
txCdsPredict score: 848.50frame shift in genome: no % Coverage: 99.40
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.