Description: SUMO1 activating enzyme subunit 1 RefSeq Summary (NM_005500): Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]. Transcript (Including UTRs) Position: hg18 chr19:52,325,984-52,405,372 Size: 79,389 Total Exon Count: 9 Strand: + Coding Region Position: hg18 chr19:52,326,028-52,404,348 Size: 78,321 Coding Exon Count: 9
ID:SAE1_HUMAN DESCRIPTION: RecName: Full=SUMO-activating enzyme subunit 1; AltName: Full=Ubiquitin-like 1-activating enzyme E1A; FUNCTION: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. PATHWAY: Protein modification; protein sumoylation. SUBUNIT: Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with UBE2I. INTERACTION: Q9UBT2:UBA2; NbExp=3; IntAct=EBI-743154, EBI-718569; SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expression level increases during S phase and drops in G2 phase (at protein level). PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: Belongs to the ubiquitin-activating E1 family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9UBE0
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0004839 ubiquitin activating enzyme activity GO:0005515 protein binding GO:0008022 protein C-terminus binding GO:0008047 enzyme activator activity GO:0008641 small protein activating enzyme activity GO:0016874 ligase activity GO:0043008 ATP-dependent protein binding GO:0044388 small protein activating enzyme binding GO:0046982 protein heterodimerization activity GO:0019948 SUMO activating enzyme activity
Biological Process: GO:0006464 cellular protein modification process GO:0016567 protein ubiquitination GO:0016925 protein sumoylation GO:0043085 positive regulation of catalytic activity GO:1903955 positive regulation of protein targeting to mitochondrion
AK021978 - Homo sapiens cDNA FLJ11916 fis, clone HEMBB1000217, highly similar to Ubiquitin-like 1-activating enzyme E1A. AK055481 - Homo sapiens cDNA FLJ30919 fis, clone FEBRA2006418, highly similar to Homo sapiens SUMO-1 activating enzyme subunit 1 (SAE1) mRNA. AK302287 - Homo sapiens cDNA FLJ58436 complete cds, highly similar to Ubiquitin-like 1-activating enzyme E1A. AK027595 - Homo sapiens cDNA FLJ14689 fis, clone NT2RP2005204, highly similar to Ubiquitin-like 1-activating enzyme E1A. BC018271 - Homo sapiens SUMO1 activating enzyme subunit 1, mRNA (cDNA clone MGC:16956 IMAGE:3452340), complete cds. BC000344 - Homo sapiens SUMO1 activating enzyme subunit 1, mRNA (cDNA clone MGC:8572 IMAGE:2823028), complete cds. AF161489 - Homo sapiens HSPC140 mRNA, complete cds. AK315624 - Homo sapiens cDNA, FLJ96708, Homo sapiens SUMO-1 activating enzyme subunit 1 (SAE1), mRNA. AF090385 - Homo sapiens SUMO-1-activating enzyme E1 N subunit (SUA1) mRNA, complete cds. AF046025 - Homo sapiens activation of Sentrin/SUMO protein AOS1 (AOS1) mRNA, complete cds. AK090645 - Homo sapiens cDNA FLJ33326 fis, clone BNGH42008943, highly similar to Homo sapiens SUMO-1 activating enzyme subunit 1 (SAE1) mRNA. BC003611 - Homo sapiens SUMO1 activating enzyme subunit 1, mRNA (cDNA clone MGC:1437 IMAGE:2988252), complete cds. AB385085 - Synthetic construct DNA, clone: pF1KB5341, Homo sapiens SAE1 gene for SUMO-activating enzyme subunit 1, complete cds, without stop codon, in Flexi system. DQ891751 - Synthetic construct clone IMAGE:100004381; FLH179581.01X; RZPDo839A03132D SUMO-1 activating enzyme subunit 1 (SAE1) gene, encodes complete protein. DQ894932 - Synthetic construct Homo sapiens clone IMAGE:100009392; FLH179577.01L; RZPDo839A03131D SUMO-1 activating enzyme subunit 1 (SAE1) gene, encodes complete protein. CU676224 - Synthetic construct Homo sapiens gateway clone IMAGE:100017052 5' read SAE1 mRNA. BT007290 - Homo sapiens SUMO-1 activating enzyme subunit 1 mRNA, complete cds. AF110956 - Homo sapiens SUMO-1 activating enzyme subunit 1 (SAE1) mRNA, complete cds. BC128518 - Homo sapiens cDNA clone IMAGE:40118709. BC128519 - Homo sapiens cDNA clone IMAGE:40118712. JD404790 - Sequence 385814 from Patent EP1572962. JD549731 - Sequence 530755 from Patent EP1572962. JD468667 - Sequence 449691 from Patent EP1572962. JD507356 - Sequence 488380 from Patent EP1572962. JD383028 - Sequence 364052 from Patent EP1572962. JD419380 - Sequence 400404 from Patent EP1572962. JD552728 - Sequence 533752 from Patent EP1572962. JD551660 - Sequence 532684 from Patent EP1572962. JD432018 - Sequence 413042 from Patent EP1572962. KJ898146 - Synthetic construct Homo sapiens clone ccsbBroadEn_07540 SAE1 gene, encodes complete protein. JD073717 - Sequence 54741 from Patent EP1572962. JD272686 - Sequence 253710 from Patent EP1572962. JD267157 - Sequence 248181 from Patent EP1572962. JD254386 - Sequence 235410 from Patent EP1572962. JD275398 - Sequence 256422 from Patent EP1572962. JD074220 - Sequence 55244 from Patent EP1572962. JD241718 - Sequence 222742 from Patent EP1572962.
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa04120 - Ubiquitin mediated proteolysis
BioCarta from NCI Cancer Genome Anatomy Project h_ctbp1Pathway - SUMOylation as a mechanism to modulate CtBP-dependent gene responses h_eradPathway - ER–associated degradation (ERAD) Pathway h_sumoPathway - Basic Mechanisms of SUMOylation
Reactome (by CSHL, EBI, and GO)
Protein Q9UBE0 (Reactome details) participates in the following event(s):