Human Gene FAM103A1 (uc002bjl.1)
  Description: hypothetical protein LOC83640
Transcript (Including UTRs)
   Position: hg18 chr15:81,445,999-81,450,427 Size: 4,429 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg18 chr15:81,448,775-81,449,823 Size: 1,049 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther Names
Model InformationMethods
Data last updated at UCSC: 2009-03-03

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr15:81,445,999-81,450,427)mRNA (may differ from genome)Protein (118 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkH-INVHGNCHPRDMGIOMIM
PubMedTreefamUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: RAM_HUMAN
DESCRIPTION: RecName: Full=RNMT-activating mini protein; Short=RAM; AltName: Full=Protein FAM103A1;
FUNCTION: Required for efficient mRNA cap methylation. Regulates RNMT expression by a post-transcriptional stabilizing mechanism.
SUBUNIT: Interacts with RNMT, enhancing its mRNA binding capacity and cap methyltransferase activity.
SUBCELLULAR LOCATION: Nucleus.
SIMILARITY: Belongs to the RAM family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  Microarray Expression Data
 
Expression ratio colors:

GNF Expression Atlas 2 Data from U133A and GNF1H Chips

      
      
      
     
    
     
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Affymetrix All Exon Microarrays

           
Ratios

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -61.54185-0.333 Picture PostScript Text
3' UTR -145.06604-0.240 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  ModBase Predicted Comparative 3D Structure on Q9BTL3
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserNo orthologNo orthologGenome BrowserNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
Gene SorterGene Sorter Gene Sorter  
MGIRGD FlyBase  
Protein Sequence  Protein Sequence  
Alignment  Alignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0004482 mRNA (guanine-N7-)-methyltransferase activity

Biological Process:
GO:0006370 7-methylguanosine mRNA capping
GO:0006397 mRNA processing
GO:0032259 methylation
GO:0036031 recruitment of mRNA capping enzyme to RNA polymerase II holoenzyme complex

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005845 mRNA cap binding complex
GO:0031533 mRNA cap methyltransferase complex


-  Descriptions from all associated GenBank mRNAs
  BC112329 - Homo sapiens family with sequence similarity 103, member A1, mRNA (cDNA clone MGC:102778 IMAGE:5578103), complete cds.
AK027095 - Homo sapiens cDNA: FLJ23442 fis, clone HSI00956.
BC003627 - Homo sapiens family with sequence similarity 103, member A1, mRNA (cDNA clone MGC:2560 IMAGE:2989772), complete cds.
BC027181 - Homo sapiens family with sequence similarity 103, member A1, mRNA (cDNA clone MGC:18029 IMAGE:3924570), complete cds.
CU693254 - Synthetic construct Homo sapiens gateway clone IMAGE:100018508 5' read FAM103A1 mRNA.
DQ892349 - Synthetic construct clone IMAGE:100004979; FLH187551.01X; RZPDo839A08150D family with sequence similarity 103, member A1 (FAM103A1) gene, encodes complete protein.
DQ895553 - Synthetic construct Homo sapiens clone IMAGE:100010013; FLH187547.01L; RZPDo839A08149D family with sequence similarity 103, member A1 (FAM103A1) gene, encodes complete protein.
AL049237 - Homo sapiens mRNA; cDNA DKFZp564B013 (from clone DKFZp564B013).
JD359342 - Sequence 340366 from Patent EP1572962.
JD371837 - Sequence 352861 from Patent EP1572962.
JD565803 - Sequence 546827 from Patent EP1572962.
KJ894890 - Synthetic construct Homo sapiens clone ccsbBroadEn_04284 FAM103A1 gene, encodes complete protein.
JD065775 - Sequence 46799 from Patent EP1572962.
JD027261 - Sequence 8285 from Patent EP1572962.
JD031002 - Sequence 12026 from Patent EP1572962.
JD338769 - Sequence 319793 from Patent EP1572962.
JD240603 - Sequence 221627 from Patent EP1572962.
JD274296 - Sequence 255320 from Patent EP1572962.
JD291954 - Sequence 272978 from Patent EP1572962.
JD137239 - Sequence 118263 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: C15orf18, F103A_HUMAN, NM_031452, NP_113640, Q2M1J8, Q9BTL3
UCSC ID: uc002bjl.1
RefSeq Accession: NM_031452
Protein: Q9BTL3 (aka RAM_HUMAN)
CCDS: CCDS10321.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_031452.2
exon count: 4CDS single in 3' UTR: no RNA size: 1220
ORF size: 357CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 914.00frame shift in genome: no % Coverage: 93.93
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.