ID:NEK6_MOUSE DESCRIPTION: RecName: Full=Serine/threonine-protein kinase Nek6; EC=2.7.11.1; AltName: Full=Never in mitosis A-related kinase 6; Short=NimA-related protein kinase 6; FUNCTION: Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, TRIP4, RPS6KB1 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence (By similarity). Phosphorylates STAT3. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. COFACTOR: Magnesium (By similarity). ENZYME REGULATION: Binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-108 (By similarity). SUBUNIT: Interacts with NEK9, predominantly in mitosis. Interacts with KIF11 (via C-terminus). Interacts with APBB1 (via WW domain). Interacts with ANKRA2, ATF4, ARHGAP33, CDC42, CIR1, PRAM1, PTN, PRDX3, PIN1, RAD26L, RBBP6, RPS7, RPS6KB1 and TRIP4 (By similarity). Interacts with STAT3. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Nucleus speckle (By similarity). Cytoplasm, cytoskeleton, centrosome (By similarity). Cytoplasm, cytoskeleton, spindle pole (By similarity). Note=Co- localizes with APBB1 at the nuclear speckles. Co-localizes with PIN1 in the nucleus. Co-localizes with ATF4, CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6, RPS7, TRIP4, RELB and PHF1 in the centrosome. Localizes to spindle microtubules in metaphase and anaphase and to the midbody during cytokinesis (By similarity). DOMAIN: Displays an autoinhibited conformation: Tyr-108 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9 (By similarity). PTM: Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210 (By similarity). SIMILARITY: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. SIMILARITY: Contains 1 protein kinase domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9ES70
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0006468 protein phosphorylation GO:0006915 apoptotic process GO:0006974 cellular response to DNA damage stimulus GO:0007049 cell cycle GO:0007059 chromosome segregation GO:0007346 regulation of mitotic cell cycle GO:0016310 phosphorylation GO:0018105 peptidyl-serine phosphorylation GO:0023014 signal transduction by protein phosphorylation GO:0030071 regulation of mitotic metaphase/anaphase transition GO:0031098 stress-activated protein kinase signaling cascade GO:0032147 activation of protein kinase activity GO:0042981 regulation of apoptotic process GO:0046777 protein autophosphorylation GO:0051301 cell division
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