Mouse Gene Hao3 (R9088)
  Description: hydroxyacid oxidase (glycolate oxidase) 3
Transcript (Including UTRs)
   Position: mm7 chr3:98,670,012-98,688,648 Size: 18,637 Total Exon Count: 9 Strand: -
Coding Region
   Position: mm7 chr3:98,670,744-98,680,266 Size: 9,523 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersMicroarray ExpressionRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Methods
Data last updated at UCSC: 2005-11-13

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr3:98,670,012-98,688,648)mRNA (may differ from genome)Protein (353 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAllen Brain Atlas
AlphaFoldBioGPSEnsemblEntrez GeneGeneCardsGeneNetwork
MGIPubMedReactomeSOURCEUniProtKB

-  Comments and Description Text from UniProtKB
  ID: HAOX2_MOUSE
DESCRIPTION: RecName: Full=Hydroxyacid oxidase 2; Short=HAOX2; EC=1.1.3.15; AltName: Full=(S)-2-hydroxy-acid oxidase, peroxisomal; AltName: Full=Medium chain alpha-hydroxy acid oxidase; AltName: Full=Medium-chain L-2-hydroxy acid oxidase;
FUNCTION: Has 2-hydroxyacid oxidase activity. Most active on medium-chain substrates.
CATALYTIC ACTIVITY: (S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2).
COFACTOR: FMN.
SUBUNIT: Homotetramer or homooctamer (By similarity).
SUBCELLULAR LOCATION: Peroxisome.
TISSUE SPECIFICITY: Pancreas.
SIMILARITY: Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.
SIMILARITY: Contains 1 FMN hydroxy acid dehydrogenase domain.
CAUTION: Was originally (PubMed:10777549) thought to originate from human.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Microarray Expression Data
 
Expression ratio colors:

GNF Expression Atlas 2 Data from GNF1M Mouse Chip

    
     
      
       
     
    
   
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Rinn et. al Sex Gene Expression Data on MOE430A Chip

        
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GNF Expression Atlas Data from U74a Mouse Chip

        
     
    
    
    
   
    
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -36.63173-0.212 Picture PostScript Text
3' UTR -149.74732-0.205 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR013785 - Aldolase_TIM
IPR012133 - Alpha-hydoxy_acid_DH_FMN
IPR000262 - FMN-dep_DH
IPR008259 - FMN_hydac_DH_AS

Pfam Domains:
PF01070 - FMN-dependent dehydrogenase

ModBase Predicted Comparative 3D Structure on Q9NYQ2
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterS. cerevisiae
Genome BrowserNo orthologNo orthologGenome BrowserGenome Browser
Gene DetailsGene Details Gene DetailsGene Details
Gene SorterGene Sorter Gene SorterGene Sorter
GenBankRGDEnsemblFlyBaseSGD
Protein Sequence  Protein SequenceProtein Sequence
Alignment  AlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003824 catalytic activity
GO:0003973 (S)-2-hydroxy-acid oxidase activity
GO:0005102 receptor binding
GO:0010181 FMN binding
GO:0016491 oxidoreductase activity
GO:0052852 very-long-chain-(S)-2-hydroxy-acid oxidase activity
GO:0052853 long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity
GO:0052854 medium-chain-(S)-2-hydroxy-acid oxidase activity

Biological Process:
GO:0018924 mandelate metabolic process
GO:0019395 fatty acid oxidation
GO:0051260 protein homooligomerization
GO:0055114 oxidation-reduction process

Cellular Component:
GO:0005739 mitochondrion
GO:0005777 peroxisome


-  Descriptions from all associated GenBank mRNAs
  AK143991 - Mus musculus adult male kidney cDNA, RIKEN full-length enriched library, clone:F530014B09 product:hydroxyacid oxidase (glycolate oxidase) 3, full insert sequence.
AK018684 - Mus musculus adult male cecum cDNA, RIKEN full-length enriched library, clone:9130419K21 product:hydroxyacid oxidase (glycolate oxidase) 3, full insert sequence.
AK078908 - Mus musculus adult male cecum cDNA, RIKEN full-length enriched library, clone:9130009N04 product:hydroxyacid oxidase (glycolate oxidase) 3, full insert sequence.
AF272947 - Mus musculus long-chain L-2-hydroxy acid oxidase (hao2) mRNA, complete cds.
AJ251820 - Mus musculus mRNA for long chain 2-hydroxy acid oxidase.
BC027754 - Mus musculus hydroxyacid oxidase (glycolate oxidase) 3, mRNA (cDNA clone MGC:29258 IMAGE:5064100), complete cds.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu00630 - Glyoxylate and dicarboxylate metabolism
mmu01100 - Metabolic pathways
mmu04146 - Peroxisome

Reactome (by CSHL, EBI, and GO)

Protein Q9NYQ2 (Reactome details) participates in the following event(s):

R-MMU-6787811 HAO2 tetramer oxidises 2OH-PALM
R-MMU-9033236 PEX5S,L:Cargo binds PEX13:PEX14 of PEX13:PEX14:PEX2:PEX10:PEX12 (Docking and Translocation Complex)
R-MMU-9033241 Peroxisomal protein import
R-MMU-390918 Peroxisomal lipid metabolism
R-MMU-392499 Metabolism of proteins
R-MMU-8978868 Fatty acid metabolism
R-MMU-556833 Metabolism of lipids
R-MMU-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: AF272947, AK018684, AK078908, AK143991, BC027754, Haox3
UCSC ID: NM_019545
RefSeq Accession: NM_019545
Protein: Q9NYQ2 (aka HAOX2_MOUSE)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.