Mouse Gene D630045J12Rik (ENSMUST00000169256.4) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus RIKEN cDNA D630045J12 gene (D630045J12Rik), mRNA. (from RefSeq NM_194061)
Gencode Transcript: ENSMUST00000169256.4
Gencode Gene: ENSMUSG00000063455.16
Transcript (Including UTRs)
   Position: mm10 chr6:38,123,176-38,254,009 Size: 130,834 Total Exon Count: 20 Strand: -
Coding Region
   Position: mm10 chr6:38,128,321-38,254,009 Size: 125,689 Coding Exon Count: 20 

Page IndexSequence and LinksUniProtKB CommentsPrimersRNA StructureProtein Structure
Other SpeciesGO AnnotationsmRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:38,123,176-38,254,009)mRNA (may differ from genome)Protein (1940 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedSOURCEUniProtKB

-  Comments and Description Text from UniProtKB
  ID: K1549_MOUSE
DESCRIPTION: RecName: Full=UPF0606 protein KIAA1549;
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).
SIMILARITY: Belongs to the UPF0606 family.
SEQUENCE CAUTION: Sequence=BAC98200.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
3' UTR -1615.705145-0.314 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR024606 - DUF3827

Pfam Domains:
PF12877 - Domain of unknown function (DUF3827)

ModBase Predicted Comparative 3D Structure on Q68FD9
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
 RGDEnsembl   
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003674 molecular_function

Biological Process:
GO:0008150 biological_process

Cellular Component:
GO:0005575 cellular_component
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  BC079889 - Mus musculus RIKEN cDNA D630045J12 gene, mRNA (cDNA clone IMAGE:30616782), partial cds.
AK128948 - Mus musculus cDNA fis, clone TRACH3007766.
AK085604 - Mus musculus 0 day neonate kidney cDNA, RIKEN full-length enriched library, clone:D630045J12 product:hypothetical protein, full insert sequence.
AK129390 - Mus musculus mRNA for mKIAA1549 protein.
AK128892 - Mus musculus cDNA fis, clone TRACH2004887, moderately similar to Homo sapiens ATPase, H+ transporting, lysosomal V0 subunit a isoform 4 (ATP6V0A4).
AF435090 - Mus musculus vacuolar proton translocating ATPase a4 isoform mRNA, complete cds.
AF326316 - Mus musculus H-ATPase accessory subunit a4 mRNA, complete cds.
AK165464 - Mus musculus adult male kidney cDNA, RIKEN full-length enriched library, clone:F530009L08 product:ATPase, H+ transporting, lysosomal V0 subunit A isoform 4, full insert sequence.
AK143790 - Mus musculus 6 days neonate spleen cDNA, RIKEN full-length enriched library, clone:F430109B04 product:ATPase, H+ transporting, lysosomal V0 subunit A isoform 4, full insert sequence.
BC042666 - Mus musculus RIKEN cDNA D630045J12 gene, mRNA (cDNA clone IMAGE:5362606), with apparent retained intron.
AK141785 - Mus musculus 9 days embryo whole body cDNA, RIKEN full-length enriched library, clone:D030069E05 product:unclassifiable, full insert sequence.
BC059853 - Mus musculus cDNA clone IMAGE:6405634, partial cds.
BC091772 - Mus musculus cDNA clone IMAGE:5008734.

-  Other Names for This Gene
  Alternate Gene Symbols: K1549_MOUSE, Kiaa1549, NM_194061, Q68FD9, Q6ZPN1, uc009bjt.1, uc009bjt.2, uc009bjt.3
UCSC ID: uc009bjt.3
RefSeq Accession: NM_194061
Protein: Q68FD9 (aka K1549_MOUSE)
CCDS: CCDS51749.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.