Gene interactions and pathways from curated databases and text-mining
Endocrinology 2001, PMID: 11316748

The insulin-like growth factor I receptor-induced interaction of insulin receptor substrate-4 and Crk-II.

Karas, M; Koval, A P; Zick, Y; LeRoith, D

Stimulation of the insulin or insulin-like growth factor (IGF)-I receptor results in activation of several signaling pathways. Proteins of the insulin receptor substrate (IRS) family play important roles in mediating these signaling cascades. To date, four members of the IRS family of docking proteins have been characterized. Recently, we have reported that stimulation of the IGF-I receptor in 293 HEK cells regulates interaction of the newly discovered IRS-4 molecule with the Crk family of proteins. In the present study, we characterize the molecular basis of these interactions. C- and N termini truncation analysis of IRS-4 demonstrated that the region between amino acids 678 and 800 of the IRS-4 molecule is involved in this interaction. This region contains a cluster of four tyrosines (Y(700), Y(717), Y(743), and Y(779)). We hypothesize that one or more of these tyrosines are involved in the interaction between the SH2 domain of the Crk-II molecule when IRS-4 is phosphorylated upon IGF-I receptor activation. Additional mutational analyses confirmed this hypothesis. Interestingly, none of these four tyrosines was individually critical for the interaction between Crk-II and IRS-4, but when all four tyrosines were simultaneously mutated to phenylalanine, the IGF-I induced interaction between these molecules was abolished. Taken together, these results suggest a novel mechanism of Crk-II binding to tyrosine phosphorylated proteins.

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Text Mining Data

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Manually curated Databases

  • IRef Biogrid Interaction: IRS4 — CRK (physical association, affinity chromatography technology)
  • IRef Hprd Interaction: IRS4 — CRK (in vitro)
  • IRef Hprd Interaction: IGF1R — IRS4 (in vivo)
  • IRef Hprd Interaction: IGF1R — IRS4 (in vitro)
  • IRef Intact Interaction: IRS4 — CRK (association, coimmunoprecipitation)
  • IRef Intact Interaction: IRS4 — CRK (association, pull down)
  • Reactome Reaction: IRS1 → IGF2 (reaction)
  • Reactome Reaction: IGF1 → IRS2 (indirect_complex)
  • Reactome Reaction: IRS2 → IRS2 (reaction)
  • Reactome Reaction: IGF1R → IRS4 (reaction)
  • Reactome Reaction: IGF1R → IGF2 (reaction)
  • Reactome Reaction: IGF1R → IRS2 (reaction)
  • Reactome Reaction: IGF1R → IRS2 (indirect_complex)
  • Reactome Reaction: IRS1 → IRS1 (reaction)
  • Reactome Reaction: IGF1R → IRS4 (indirect_complex)
  • Reactome Reaction: IGF1 → IRS1 (reaction)
  • Reactome Reaction: IGF1 → IGF1 (reaction)
  • Reactome Reaction: IGF1 → IRS1 (indirect_complex)
  • Reactome Reaction: IGF1 → IRS4 (reaction)
  • Reactome Reaction: IRS2 → IGF2 (reaction)
  • Reactome Reaction: IRS1 → IRS4 (reaction)
  • Reactome Reaction: IRS1 → IRS2 (reaction)
  • Reactome Reaction: IGF1 → IRS4 (indirect_complex)
  • Reactome Reaction: IGF1 → IRS2 (reaction)
  • Reactome Reaction: IGF2 → IGF2 (reaction)
  • Reactome Reaction: IGF1 → IGF2 (reaction)
  • Reactome Reaction: IRS1 → IGF2 (indirect_complex)
  • Reactome Reaction: IGF1R → IGF1R (reaction)
  • Reactome Reaction: IRS2 → IGF2 (indirect_complex)
  • Reactome Reaction: IRS4 → IGF2 (reaction)
  • Reactome Reaction: IRS4 → IRS4 (reaction)
  • Reactome Reaction: IRS2 → IRS4 (reaction)
  • Reactome Reaction: IGF1R → IRS1 (indirect_complex)
  • Reactome Reaction: IGF1R → IRS1 (reaction)
  • Reactome Reaction: IGF1R → IGF1 (reaction)
  • Reactome Reaction: IRS4 → IGF2 (indirect_complex)
In total, 22 gene pairs are associated to this article in curated databases