Gene interactions and pathways from curated databases and text-mining
Pediatr Allergy Immunol 2007, PMID: 17617806

Suppressive effects of ketotifen on Th1- and Th2-related chemokines of monocytes.

Hung, Chih-Hsing; Suen, Jau-Ling; Hua, Yi-Ming; Chiang, Wen; Chang, Hui-Chiu; Chen, Chun-Nan; Jong, Yuh-Jyh

Ketotifen is a mast cell stabilizer and useful in younger children with allergic diseases such as asthma and allergic rhinitis. Macrophage-derived chemokine (MDC) is a T-helper cell type 2 (Th2)-related chemokine involved in recruitment of Th2 cells toward allergen-challenged inflammation. However, the Th1-related chemokines, interferon-inducible protein 10 (IP-10)/CXCL10, and the monokine induced by interferon-gamma (MIG)/CXCL9 are also important in allergen-induced asthma in animal models. We investigated the effects of ketotifen on the expression of Th1- and Th2-related chemokines of human monocytes in vitro and ex vivo. Ketotifen (5-50 microM) significantly down-regulated lipopolysaccharide (LPS)-induced MDC, MIG and IP-10 (p < 0.05, each comparison) in THP-1 cells and human primary monocytes in a dose-dependent manner. SB203580 [p38-mitogen-activated protein kinase (MAPK) inhibitor] suppressed LPS-induced MDC and IP-10 expression, and PD98059 (ERK-MAPK inhibitor) could only suppress LPS-induced IP-10, but not MDC expression. LPS-induced pp38 and p-ERK expression of THP-1 monocytic cells was suppressed by ketotifen. These data demonstrate that ketotifen is effective in down-regulating LPS-induced MDC, MIG and IP-10, which play important roles in the pathogenesis of airway inflammation. The suppressive effect on MDC and IP-10 may, at least in part, involve the down-regulation of LPS-induced p38 and ERK-MAPK expression.

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Text Mining Data

IP-10 → lipopolysaccharide (LPS): " Ketotifen ( 5-50 microM ) significantly down-regulated lipopolysaccharide (LPS) induced MDC, MIG and IP-10 ( p < 0.05, each comparison ) in THP-1 cells and human primary monocytes in a dose dependent manner "

MDC → lipopolysaccharide (LPS): " Ketotifen ( 5-50 microM ) significantly down-regulated lipopolysaccharide (LPS) induced MDC , MIG and IP-10 ( p < 0.05, each comparison ) in THP-1 cells and human primary monocytes in a dose dependent manner "

MIG → lipopolysaccharide (LPS): " Ketotifen ( 5-50 microM ) significantly down-regulated lipopolysaccharide (LPS) induced MDC, MIG and IP-10 ( p < 0.05, each comparison ) in THP-1 cells and human primary monocytes in a dose dependent manner "

IP-10 → LPS: " SB203580 [ p38-mitogen activated protein kinase ( MAPK ) inhibitor ] suppressed LPS induced MDC and IP-10 expression, and PD98059 ( ERK-MAPK inhibitor ) could only suppress LPS induced IP-10 , but not MDC expression "

MDC → LPS: " SB203580 [ p38-mitogen activated protein kinase ( MAPK ) inhibitor ] suppressed LPS induced MDC and IP-10 expression, and PD98059 ( ERK-MAPK inhibitor ) could only suppress LPS induced IP-10, but not MDC expression "

p-ERK → LPS: " LPS induced pp38 and p-ERK expression of THP-1 monocytic cells was suppressed by ketotifen "

MDC → LPS: " These data demonstrate that ketotifen is effective in down regulating LPS induced MDC , MIG and IP-10, which play important roles in the pathogenesis of airway inflammation "

IP-10 ⊣ p38: " The suppressive effect on MDC and IP-10 may, at least in part, involve the down-regulation of LPS induced p38 and ERK-MAPK expression "

IP-10 ⊣ ERK-MAPK: " The suppressive effect on MDC and IP-10 may, at least in part, involve the down-regulation of LPS induced p38 and ERK-MAPK expression "

IP-10 ⊣ ERK-MAPK: " The suppressive effect on MDC and IP-10 may, at least in part, involve the down-regulation of LPS induced p38 and ERK-MAPK expression "

MDC ⊣ p38: " The suppressive effect on MDC and IP-10 may, at least in part, involve the down-regulation of LPS induced p38 and ERK-MAPK expression "

MDC ⊣ ERK-MAPK: " The suppressive effect on MDC and IP-10 may, at least in part, involve the down-regulation of LPS induced p38 and ERK-MAPK expression "

MDC ⊣ ERK-MAPK: " The suppressive effect on MDC and IP-10 may, at least in part, involve the down-regulation of LPS induced p38 and ERK-MAPK expression "

p38 → LPS: " The suppressive effect on MDC and IP-10 may, at least in part, involve the down-regulation of LPS induced p38 and ERK-MAPK expression "

ERK-MAPK → LPS: " The suppressive effect on MDC and IP-10 may, at least in part, involve the down-regulation of LPS induced p38 and ERK-MAPK expression "

ERK-MAPK → LPS: " The suppressive effect on MDC and IP-10 may, at least in part, involve the down-regulation of LPS induced p38 and ERK-MAPK expression "

Manually curated Databases

No curated data.