Gene interactions and pathways from curated databases and text-mining
Scientific reports 2013, PMID: 23604351

Analysis of SUMOylated proteins using SUMO-traps.

Da Silva-Ferrada, Elisa; Xolalpa, Wendy; Lang, Valérie; Aillet, Fabienne; Martin-Ruiz, Itziar; de la Cruz-Herrera, Carlos F; Lopitz-Otsoa, Fernando; Carracedo, Arkaitz; Goldenberg, Seth J; Rivas, Carmen; England, Patrick; Rodríguez, Manuel S

SUMO-modified proteins are recognized by SUMO interacting motifs (SIMs), thus triggering diverse cellular responses. Here SIMs were used to develop SUMO-traps to capture endogenous SUMOylated proteins. Our results show that these small peptides are transferable motifs that maintain their SUMO binding capacity when fused to the heterologous carrier protein GST. The tandem disposition of SIMs increases the binding capacity of SUMO-traps to specifically interact with polySUMO but not poly-Ubiquitin chains. We demonstrate that this SUMO capturing system purifies SUMOylated proteins such as IκBα, PTEN, PML or p53 in vitro and in vivo. These properties can be used to explore the many critical functions regulated by protein SUMOylation.

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Text Mining Data

Dashed line = No text mining data

Manually curated Databases

  • IRef Biogrid Interaction: SUMO1 — TP53 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SUMO3 — TP53 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NFKBIA — SUMO3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PTEN — SUMO1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PTEN — SUMO3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SUMO1 — PML (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NFKBIA — SUMO1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SUMO3 — PML (physical association, affinity chromatography technology)
In total, 8 gene pairs are associated to this article in curated databases