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IGF1 — VEGFA

Text-mined interactions from Literome

Bermont et al., Int J Cancer 2000 (Adenocarcinoma...) : Regulation of vascular endothelial growth factor expression by insulin-like growth factor-I in endometrial adenocarcinoma cells ... Our aim was firstly to determine the role of Insulin-like Growth Factor-I (IGF-I) in the regulation of VEGF expression in endometrial adenocarcinoma cells and then the mechanism by which this regulation occurs ... Our results suggest that IGF-I regulates VEGF expression in endometrial adenocarcinoma cells at the post-transcriptional level by enhancing the stabilization of the 2 major VEGF isoform mRNAs ( VEGF ( 121 ) and VEGF ( 165 ) ) ... In addition to its proliferative functions, IGF-I induces VEGF expression and participates in the maintenance of an angiogenic phenotype ... In addition to its proliferative functions, IGF-I induces VEGF expression and participates in the maintenance of an angiogenic phenotype
Miele et al., J Biol Chem 2000 : Insulin and insulin-like growth factor-I induce vascular endothelial growth factor mRNA expression via different signaling pathways ... The same elevated levels of mRNA were maintained after 24 h of stimulation with insulin, whereas IGF-I treatment further increased VEGF mRNA expression to 12-fold after 24 h. Pre-incubation with the phosphatidylinositol 3-kinase inhibitor wortmannin abolished the effect of insulin on VEGF mRNA expression in NIH-IR cells but did not modify the IGF-I induced VEGF mRNA expression in NIH-IGFR cells ... Blocking mitogen activated protein kinase activation with the MEK inhibitor PD98059 abolished the effect of IGF-I on VEGF mRNA expression in NIH-IGFR cells but had no effect on insulin induced VEGF mRNA expression in NIH-IR cells ... We conclude that VEGF induction by insulin and IGF-I occurs via different signaling pathways, the former involving phosphatidylinositol 3-kinase/protein kinase B and the latter involving MEK/mitogen activated protein kinase
Deckers et al., Endocrinology 2000 : VEGF-A production during osteoblast differentiation was stimulated by insulin-like growth factor I that enhances osteoblast differentiation and was inhibited by PTH related peptide that inhibits osteoblast differentiation
Nagai et al., Horm Metab Res 2000 (Acromegaly...) : It was recently found that insulin-like growth factor-I (IGF-I) enhances VEGF gene expression
Liu et al., Biochem Biophys Res Commun 2001 : IGF-I also stimulated VEGF secretion from CSMC at an optimal concentration of 10 ng/ml
Hellstrom et al., Proc Natl Acad Sci U S A 2001 (Retinopathy of Prematurity) : Low IGF-I suppresses VEGF-survival signaling in retinal endothelial cells : direct correlation with clinical retinopathy of prematurity
Clarke et al., Br J Cancer 2001 (Cell Transformation, Neoplastic) : Mutant epidermal growth factor receptor enhances induction of vascular endothelial growth factor by hypoxia and insulin-like growth factor-1 via a PI3 kinase dependent pathway
Schams et al., Exp Clin Endocrinol Diabetes 2001 : Stimulatory and synergistic effects of luteinising hormone and insulin like growth factor 1 on the secretion of vascular endothelial growth factor and progesterone of cultured bovine granulosa cells
Akeno et al., Endocrinology 2002 : Induction of vascular endothelial growth factor by IGF-I in osteoblast-like cells is mediated by the PI3K signaling pathway through the hypoxia-inducible factor-2alpha ... Pretreatment of the cells with LY294002 also greatly attenuated IGF-I induction of HIF-2alpha and blunted IGF-I induced VEGF promoter activity ... These data indicate that IGF-I , by activation of the PI3K pathway, induces VEGF expression in osteoblasts through a transcriptional control mechanism common to those that activate VEGF and other hypoxia response genes
Wu et al., Cancer Res 2002 (Adenocarcinoma...) : The expression of vascular epithelial growth factor (VEGF) as well as vessel abundance in the cecum tumors was dependent on the levels of serum IGF-I
Reinmuth et al., Clin Cancer Res 2002 (Colonic Neoplasms...) : RESULTS : IGF-I increased VEGF expression in parental and vector transfected cells, whereas IGF-I induction of VEGF mRNA and protein was abrogated in IGF-IR DN cells
Burroughs et al., Mol Cancer Res 2003 (MAP Kinase Signaling System) : Phosphatidylinositol 3-kinase and mek1/2 are necessary for insulin-like growth factor-I induced vascular endothelial growth factor synthesis in prostate epithelial cells : a role for hypoxia-inducible factor-1 ? ... IGF-I increased the secretion of VEGF ( 165 ) into PrEC growth medium and stimulated transcription of a reporter gene driven by a 1.5-kb region of the VEGF promoter ... Inhibition of either phosphatidylinositol 3-kinase (PI3-K) or Mek1/2 signaling pathways completely abrogated the IGF-I induced increase in VEGF secretion and promoter activity, indicating a dependence on coordinate signaling from both pathways to produce this effect ... The expression of an activator protein (AP)-1 dominant negative in an immortalized prostate epithelial cell line PZ-HPV-7 suppressed the IGF-I induced increase in VEGF promoter activity ... Mutation of the hypoxia response element ( HRE ), which mediates hypoxic stimulation of VEGF transcription, did not inhibit the effect of IGF-I on the VEGF promoter, despite the fact that this mutation inhibited PI3-K stimulated VEGF promoter activity in prostate cancer cells
Gruden et al., Kidney Int 2003 : IGF-I induces vascular endothelial growth factor in human mesangial cells via a Src dependent mechanism ... We investigated whether IGF-I affects VEGF gene expression and protein secretion in human mesangial cells ... IGF-I induced a dose dependent increase in VEGF protein levels ( 10 ( -11 ) mol/L, 5 % ; 10 ( -10 ) mol/L, 14 % ; 10 ( -9 ) mol/L, 46 % ; 10 ( -8 ) mol/L, 66 % ; 10 ( -7 ) mol/L, 68 % ; P < 0.001 ) ... IGF-I induced VEGF production rose by 6 hours with a peak at 12 hours, and declined by 24 hours ( 52 %, 72 %, and 34 %, respectively ; P < 0.01 at 12 hours ) ... IGF-I induced VEGF protein secretion was not affected by the addition of wortmannin ( IGF-I, 76 % vs. IGF-I + wortmannin, 79 % increase over control ; P = NS ), but was abolished by alpha IR3 ( IGF-I, 69 % vs. IGF-I +alpha IR3, 0 % ; P < 0.001 ) and significantly reduced by PP2 ( IGF-I, 50 % vs. IGF-I + PP2, 14 % ; P < 0.01 ) ... IGF-I induces VEGF gene expression and protein secretion in human mesangial cells via a Src dependent mechanism
Poulaki et al., J Clin Endocrinol Metab 2003 (Adenocarcinoma...) : Regulation of vascular endothelial growth factor expression by insulin-like growth factor I in thyroid carcinomas ... We found that IGF-I up-regulated VEGF mRNA expression and protein secretion ... The IGF-I induced up-regulation of VEGF production was associated with activation of AP-1 and HIF-1 alpha and was abrogated by phosphatidylinositol 3-kinase inhibitors ( wortmannin and LY294002 ) ; Jun kinase inhibitor ( SP600125 ) ; HIF-1 alpha antisense oligonucleotide ; or geldanamycin, an inhibitor of the heat shock protein 90 molecular chaperone, which regulates the three-dimensional conformation and function of IGF-I-receptor and Akt ... These data indicate that IGF-I stimulates VEGF synthesis in thyroid carcinomas in an Akt dependent pathway via AP-1 and HIF-1 alpha and provide the framework for clinical use of small-molecule inhibitors, including geldanamycin analogs, to abrogate proangiogenic cascades in thyroid cancer
Neid et al., J Biol Chem 2004 (Adenocarcinoma...) : Previously we have shown that in AsPC-1 pancreatic adenocarcinoma cells, insulin-like growth factor receptor ( IGF-IR ) regulates VPF/VEGF expression
Strammiello et al., Horm Metab Res 2003 (Bone Neoplasms...) : IGF-I increases expression of VEGF-A , TGFbeta, CTGF and Cyr61 mRNA ... However, only the modulation of VEGF-A expression appears to be mediated by IGF-IR ... Analysis of the signaling mechanisms involved in constitutive or IGF induced expression and secretion of VEGF-A indicated that PI3-K and MAPK signaling pathways are both required for VEGF expression and production in ES cells
Menu et al., Br J Cancer 2004 (Disease Models, Animal...) : Stimulation by IGF-1 of VEGF production was reduced by PD98059, indicating that only the MEK-ERK pathway is involved in IGF-1 stimulated VEGF production
Ruberte et al., J Clin Invest 2004 (Cataract...) : Neovascularization was consistent with increased IGF-1 induction of VEGF expression in retinal glial cells
Laron et al., Eur J Endocrinol 2004 (Diabetes Mellitus, Type 2...) : Our findings show that congenital IGF-I deficiency, similar to excess, causes vascular complications of DM, denoting also that vascular endothelial growth factor can induce neovascularization in the presence of congenital IGF-I deficiency
Sall et al., Exp Eye Res 2004 : Somatostatin inhibits IGF-1 mediated induction of VEGF in human retinal pigment epithelial cells ... Neovascularization stimulated by IGF-1 mediated induction of vascular endothelial growth factor ( VEGF ) is one of the leading causes of blindness in humans ... IGF-1 induced a dose dependent increase in IGF-1R phosphorylation and in VEGF mRNA levels in cultured human RPE cells
Rosenthal et al., Biochem Biophys Res Commun 2004 (Choroidal Neovascularization...) : Thus, in RPE cells IGF-1 stimulates the second messenger Ca ( 2+ ) and increases VEGF secretion which, in turn, induces neovascularization
Stearns et al., Cancer Res 2005 (Neovascularization, Pathologic...) : Activated Ras enhances insulin-like growth factor I induction of vascular endothelial growth factor in prostate epithelial cells ... We find that, in prostate epithelial cells, the introduction of an activated HRAS causes cells to produce VEGF in response to insulin-like growth factor I (IGF-I) ... In comparison, cells lacking an activated Ras are unable to produce VEGF in response to IGF-I
Smith et al., Biol Neonate 2005 (Birth Weight...) : IGF-1 is required for maximum VEGF activation of vascular endothelial cell proliferation and survival pathways
Beckert et al., Biochem Biophys Res Commun 2006 : IGF-I induced VEGF expression in HUVEC involves phosphorylation and inhibition of poly ( ADP-ribose ) polymerase ... We first demonstrated that IGF-I increased VEGF protein expression in endothelial cells
Slomiany et al., Biochem Biophys Res Commun 2006 (Carcinoma, Squamous Cell...) : IGF-1 induced vascular endothelial growth factor secretion in head and neck squamous cell carcinoma
Treins et al., Biochem Biophys Res Commun 2006 : AICAR stimulated VEGF mRNA expression, but did not modify the insulin- and IGF-1 induced VEGF expression ... These results show that AICAR and insulin/IGF-1 regulate VEGF expression through different mechanisms
Nakamura et al., Cancer Res 2006 (MAP Kinase Signaling System...) : Recently, we have shown that insulin-like growth factor-I and serum derived growth factors stimulate VEGF expression in neuroblastoma cells via induction of hypoxia-inducible factor-1alpha ( HIF-1alpha )
Slomiany et al., J Pharmacol Exp Ther 2006 (Anoxia) : Hypoxia-inducible factor-1 dependent and -independent regulation of insulin-like growth factor-1 stimulated vascular endothelial growth factor secretion ... However, IGF-1 stimulated a more significant increase in VEGF secretion ... IGF-1 stimulated VEGF promoter activity was phosphatidylinositol 3-kinase (PI3K)/Akt/mTOR ( mammalian target of rapamycin ) -dependent, whereas VEGF secretion was only partially reduced by inhibition of PI3K/Akt/mTOR and HIF-1 activities ... In conclusion, IGF-1 regulates VEGF expression and secretion via HIF-1 dependent and -independent pathways
Carroll et al., Cancer Res 2006 (Breast Neoplasms...) : We found that HIF-1alpha primarily regulates transcriptional activation of VEGF in response to hypoxia and IGF-I compared with HIF-2alpha in MCF-7 cells
Wu et al., Cancer Immunol Immunother 2007 (Disease Models, Animal...) : Inhibition of IGF-IR by the A12 in vitro suppressed both constitutive and IGF-I induced secretion of VEGF , indicating that a putative anti-angiogenic mechanism associated with the A12 treatment may contribute to its anti-tumor effect
Jiang et al., J Exp Med 2007 (Cardiomyopathy, Hypertrophic...) : Both IGF-1 and Cu activate hypoxia-inducible factor (HIF)-1alpha and HIF-1alpha gene silencing blocks IGF-1- or Cu-stimulated VEGF expression
Stanek et al., J Clin Endocrinol Metab 2007 (Polycystic Ovary Syndrome) : However, insulin or IGF increased VEGF-A levels within 1 d and appeared to synergize with LH at 3 d. VEGF-A production by non-PCOS LGCs was more sensitive to IGF exposure, whereas PCOS cells were more sensitive to insulin
Jeschke et al., Gene Ther 2007 : IGF-I , KGF and their combination cDNA treatment significantly ( P < 0.05 ) accelerated re-epithelization, increased IGF-I, KGF, FGF, VEGF and collagen type IV expression, while it had no effect on collagen type I and III expression
Bauer et al., Ann Surg Oncol 2007 (Colorectal Neoplasms...) : The effect of anti-IGF-IR MoAB on IGF-I induced vascular endothelial growth factor ( VEGF ) production in human CRC cells was assessed by Northern blot and ELISA ... IGF-I stimulation of CRC cells resulted in significant upregulation of VEGF and this was completely inhibited by pretreatment with anti-IGF-IR MoAB
Sadat et al., Biochem Biophys Res Commun 2007 : Cardiomyocytes apoptosis was significantly prevented by ASCs and 62.5 % of the anti-apoptotic effect was mediated by IGF-I and 34.2 % by VEGF
Economou et al., Invest Ophthalmol Vis Sci 2008 (Choroidal Neovascularization...) : PPP reduces IGF-1 dependent VEGF expression and CNV in vivo
Economou et al., Acta Ophthalmol 2008 (Choroidal Neovascularization) : Conclusions : PPP reduces IGF-1 dependent VEGF expression and CNV in vivo
Rudnicki et al., Lab Invest 2009 (Kidney Diseases...) : In vitro experiments confirmed the positive regulatory effect of EGF and IGF-1 on VEGF-A transcription in human proximal tubular cells
Tang et al., Acta Pharmacol Sin 2009 (Breast Neoplasms) : To investigate the effects of dauricine ( Dau ) on insulin-like growth factor-I (IGF-I) induced hypoxia inducible factor 1alpha ( HIF-1alpha ) and vascular endothelial growth factor ( VEGF ) expression in human breast cancer cells ( MCF-7 ) ... However, Dau remarkably suppressed VEGF expression at both protein and mRNA levels in response to IGF-I ... Mechanistically, Dau suppressed IGF-I induced HIF-1alpha and VEGF protein expression mainly by blocking the activation of PI-3K/AKT/mTOR signaling pathway
Haurigot et al., J Biol Chem 2009 (Gliosis) : High intraocular IGF-I , resulting from overexpression of IGF-I in the retina, increased IGF-I receptor content and signaling and led to accumulation of vascular endothelial growth factor
Ryan et al., Br J Cancer 2009 (Adenocarcinoma...) : Proliferation and vascular endothelial growth factor ( VEGF ) production by 4T1.2 cells was increased by IGF1 ( E3R ) ( recombinant IGF1 resistant to binding by IGFBPs ) but not by wild-type IGF1
Ainscough et al., Exp Eye Res 2009 : In the course of studying the effects of IGFBPs on IGF mediated VEGF secretion and Vn-mediated phagocytosis in the RPE cell line ARPE-19, we have discovered that these cells avidly ingest synthetic microspheres ( 2.0 mum diameter ) coated with IGFBPs
Tang et al., Int J Cancer 2010 (Breast Neoplasms...) : We tested whether bisphosphonates had any effects on hypoxia-inducible factor (HIF)-1alpha/vascular endothelial growth factor ( VEGF ) axis that plays a pivotal role in tumor angiogenesis, and our results showed that both pamidronate and clodronate significantly suppressed IGF-1 induced HIF-1alpha protein accumulation and VEGF expression in MCF-7 cells
Zisa et al., Am J Physiol Regul Integr Comp Physiol 2009 (Disease Models, Animal...) : VEGF also induced production of HGF, IGF2 , and VEGF by skeletal muscle
Sulkowski et al., Clin Chem Lab Med 2009 (Colorectal Neoplasms) : IGF-I induces expression of VEGF and decomposes wild type p53 in cancer cell lines ... Serum concentrations of IGF-I and VEGF did not show positive correlation, as expected due to IGF-I induction of VEGF in malignant colon cell lines
Wakabayashi et al., J Neurosci Res 2010 (Brain Ischemia...) : In MSC transplanted brain, among many neurotrophic factors, only human insulin-like growth factor 1 (IGF-1) was detected in the core and ischemic border zone at 3 days after MCAO, whereas host cells expressed markedly higher neurotrophic factors ( rat origin ) than control rats, especially vascular endothelial growth factor ( VEGF ) at 3 days and epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) at 7 days after MCAO
Weng et al., Curr Eye Res 2009 (MAP Kinase Signaling System...) : There was also an IGF-1 induced increase in VEGF synthesis as measured quantitatively by [ ( 14 ) C ] methionine-VEGF immunoprecipitation ... PD98059 suppressed both IGF-1 induced cell proliferation as well as IGF-1 stimulated VEGF production ... These studies suggest that IGF-1 is a mitogen for hRPE cells and also stimulates production of the angiogenic factor, VEGF
Thamm et al., Melanoma Res 2010 (Dog Diseases...) : IGF-1 stimulated cell proliferation and vascular endothelial growth factor production in 17CM98, and addition of the IGF-1R inhibitor ADW742 abrogated IGF-1 induced phenotypic changes
Cavallaro et al., World J Surg 2010 (Adenoma...) : IGF-1 stimulation resulted in 5.4 +/- 0.65 cells, bFGF stimulation in 5.68 +/- 0.86 cells, and VEGF stimulation resulted in 6.03 +/- 1.03 cells
Spirli et al., Hepatology 2010 (Cysts...) : We have hypothesized that mammalian target of rapamycin (mTOR) represents a common pathway for the regulation of hypoxia-inducible factor 1 alpha ( HIF1alpha ) -dependent VEGF secretion by IGF1 and ERK1/2 ... Rapamycin inhibited IGF1 stimulated HIF1alpha accumulation and VEGF secretion in LCECs
Zhang et al., BMC cancer 2010 (Colorectal Neoplasms...) : In addition, IGF-I could effectively induce the VEGF and VEGF-C mRNA expression and protein secretion in colorectal cancer cells expressing IGFIR molecules ... In addition, IGF-I could effectively induce the VEGF and VEGF-C mRNA expression and protein secretion in colorectal cancer cells expressing IGFIR molecules
Gariboldi et al., Biochem Pharmacol 2010 (Glioblastoma) : Our results show that exogenous IGF-I causes IGF1R and STAT3 activation, and increases HIF1alpha protein levels and HIF1 trascriptional activity, inducing VEGF release ; a similar response, mediated by IGF-II release, is observed following HIF1alpha stabilization
Spiliotaki et al., Lung Cancer 2011 (Carcinoma, Non-Small-Cell Lung...) : Moreover, inhibition of IGF-IR signaling by AVE1642 reduced IGF-I induced VEGF production by NSCLC cells as well as the migratory capacity of HUVEC cells challenged with conditioned media from lung cancer cells previously exposed to IGF-I
Li et al., Cancer 2011 (Neovascularization, Pathologic...) : In addition, it has been demonstrated that IGF-IR signaling affects vascular endothelial growth factor ( VEGF ) expression in some other types of tumors ... IGF-IR/dn reduced VEGF expression, reduced the activation of both protein kinase B ( Akt ) and mitogen activated protein kinase ( MAPK ), and reduced vascular formation, indicating that IGF-IR/dn inhibited tumor growth in mice by inhibiting both angiogenesis and lymphangiogenesis
Piecewicz et al., PloS one 2012 : We confirmed that IGF-IGFR1 signaling stabilizes HIF1a and leads to up-regulation of VEGF during vasculogenesis in embryoid bodies
Goel et al., Cancer Discov 2012 (Prostatic Neoplasms) : VEGF/NRP2 signaling represses insulin-like growth factor-1 receptor ( IGF-IR ) expression and signaling, and the mechanism involves Bmi-1 mediated transcriptional repression of the IGF-IR
Jiang et al., Ann Clin Lab Sci 2013 (Diabetes Mellitus, Type 2) : The role of insulin-like growth factor I and hypoxia inducible factor 1a in vascular endothelial growth factor expression in type 2 diabetes ... However, little is known about the role of HIF-1a/ IGF-I in VEGF expression in patients with diabetic vascular complications
Goad et al., Endocrinology 1996 : In human SaOS-2 osteoblast-like cells, 10 nM IGF-I increased the abundance of VEGF messenger RNA ( mRNA ) by 4-fold above the control value at 2h, and the elevated levels of mRNA returned to near basal by 8 h. IGF-I stimulated VEGF mRNA levels at IGF-I concentrations as low as 1-2 nM ... We conclude that IGF-I enhances osteoblast synthesis of VEGF , which may then act locally on endothelium to stimulate angiogenesis, an essential component of bone growth and remodeling
Wang et al., J Bone Miner Res 1996 (Bone Neoplasms...) : We have demonstrated that PTH, IGF-I , and most potently 1,25- ( OH ) 2D3 stimulate the mRNA expression and secretion of VEGF in human osteoblast-like cells, suggesting that one of the anabolic effects of 1,25- ( OH ) 2D3 on skeletal tissue may be mediated by VEGF produced by osteoblasts
Punglia et al., Diabetes 1997 : Regulation of vascular endothelial growth factor expression by insulin-like growth factor I ... Further, hypoxia mediated and IGF-I mediated increases in VEGF mRNA and protein levels were additive in RPE cells, and the hypoxia induced VEGF increases were independent of endogenous IGF-I ... VEGF promoter activity was enhanced by IGF-I in RPE cells, but VEGF transcript half-life was unaltered
Akagi et al., Cancer Res 1998 (Colonic Neoplasms) : Regulation of vascular endothelial growth factor expression in human colon cancer by insulin-like growth factor-I ... We investigated the role of insulin-like growth factor (IGF)-I and IGF binding proteins ( IGFBPs ) in the regulation of vascular endothelial growth factor ( VEGF ) expression in colon cancer cells and the mechanism by which this regulation occurs ... We investigated the role of insulin-like growth factor (IGF)-I and IGF binding proteins ( IGFBPs ) in the regulation of vascular endothelial growth factor ( VEGF ) expression in colon cancer cells and the mechanism by which this regulation occurs ... IGF-I induction of VEGF was confirmed at the protein level ... In experiments using transient transfection of VEGF promoter-reporter constructs into HT29 cells, IGF-I increased the activity of the VEGF promoter, and pretreatment of HT29 cells with dactinomycin abrogated the induction of VEGF mRNA by IGF-I ... These findings suggest that IGF-I regulates VEGF expression in human colon cancer cells by induction of transcription of the VEGF gene ... IGFBPs do not significantly affect IGF-I induction of VEGF