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CDC42 — WASL

Pathways - manually collected, often from reviews:

• KEGG Adherens junction: CDC42 → WASL (protein-protein, activation)
• KEGG Adherens junction: CDC42 → WAS/WASL (protein-protein, activation)
• KEGG Fc gamma R-mediated phagocytosis: CDC42 → Complex of VASP-WAS-WASL (protein-protein, activation)
• KEGG Regulation of actin cytoskeleton: CDC42 → WAS/WASL (protein-protein, activation)
• KEGG Bacterial invasion of epithelial cells: CDC42 → WAS/WASL (protein-protein, activation)
• KEGG Bacterial invasion of epithelial cells: CDC42 → WAS/WASL (protein-protein, activation)
• KEGG Shigellosis: WAS/WASL → CDC42 (protein-protein, binding/association)
• KEGG Chemokine signaling pathway: CDC42 → WAS/WASL (protein-protein, activation)
• NCI Pathway Database CDC42 signaling events: CDC42/GTP complex (CDC42) → N-WASP (WASL) (modification, activates) Rohatgi et al., Cell 1999, Higgs et al., J Cell Biol 2000, Symons et al., Cell 1996*, Kolluri et al., Proc Natl Acad Sci U S A 1996*
  Evidence: assay, physical interaction
• NCI Pathway Database EPHB forward signaling: CDC42/GDP complex (CDC42) → Ephrin B/EPHB2/Intersectin/N-WASP complex (EFNB3_EFNB2_EFNB1-EPHB2-ITSN1-WASL) (modification, collaborate)
  Irie et al., Nat Neurosci 2002
  Evidence: mutant phenotype, assay
• NCI Pathway Database CDC42 signaling events: CDC42/GTP/IRSP53 complex (CDC42-BAIAP2) → N-WASP (WASL) (modification, collaborate) Lim et al., J Biol Chem 2008
  Evidence: assay, physical interaction
• NCI Pathway Database CDC42 signaling events: CDC42/GTP/IRSP53 complex (CDC42-BAIAP2) → CDC42/GTP/IRSP53/N-WASP complex (CDC42-BAIAP2-WASL) (modification, collaborate)
  Lim et al., J Biol Chem 2008
  Evidence: assay, physical interaction
• NCI Pathway Database CDC42 signaling events: N-WASP (WASL) → CDC42/GTP/IRSP53/N-WASP complex (CDC42-BAIAP2-WASL) (modification, collaborate)
  Lim et al., J Biol Chem 2008
  Evidence: assay, physical interaction
• NCI Pathway Database Netrin-mediated signaling events: CDC42/GTP complex (CDC42) → N-WASP (WASL) (modification, activates) Shekarabi et al., J Neurosci 2005
  Evidence: physical interaction
• Reactome Reaction: WASL → CDC42 (reaction) Rohatgi et al., J Cell Biol 2000*, Millard et al., Biochem J 2004*
• Reactome Reaction: WASL → CDC42 (indirect_complex) Carlier et al., J Biol Chem 2000, Moreau et al., Nat Cell Biol 2000, Rohatgi et al., J Cell Biol 2000*, Fukuoka et al., J Cell Biol 2001, Rohatgi et al., J Biol Chem 2001, Ho et al., Proc Natl Acad Sci U S A 2001, Kato et al., Biochem Biophys Res Commun 2002, Aspenström et al., Exp Cell Res 2002, Cory et al., J Biol Chem 2002, Wu et al., J Biol Chem 2004, Millard et al., Biochem J 2004*
• WikiPathways G13 Signaling Pathway: CDC42 → WASL/WAS (activation)
• WikiPathways Chemokine signaling pathway: CDC42 → WAS/WASL (activation)

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Biogrid Interaction: WASL — CDC42 (direct interaction, two hybrid) Bandyopadhyay et al., Nat Methods 2010
• IRef Biogrid Interaction: WASL — CDC42 (direct interaction, pull down) Martinez-Quiles et al., Nat Cell Biol 2001*
• IRef Biogrid Interaction: WASL — CDC42 (direct interaction, pull down) Carlier et al., J Biol Chem 2000
• IRef Biogrid Interaction: WASL — CDC42 (direct interaction, pull down) Miki et al., Nature 1998*
• IRef Hprd Interaction: CDC42 — WASL (in vitro) Kim et al., Nature 2000*, Hüfner et al., FEBS Lett 2002*, Miki et al., Nature 1998*
• IRef Intact Interaction: WASL — CDC42 (physical association, two hybrid pooling approach) Bandyopadhyay et al., Nat Methods 2010
• IRef Intact Interaction: WASL — CDC42 (physical association, coimmunoprecipitation) Tang et al., J Biol Chem 2005*
• IRef Intact Interaction: Complex of CDC42-LCK-WASL (phosphorylation reaction, protein kinase assay) Torres et al., J Biol Chem 2006*
• IRef Intact Interaction: Complex of CDC42-PTPN2-WASL (phosphorylation reaction, phosphatase assay) Torres et al., J Biol Chem 2006*
• IRef Ophid Interaction: WASL — CDC42 (aggregation, interologs mapping) Brown et al., Bioinformatics 2005

Text-mined interactions from Literome

Miki et al., Nature 2000 : N-WASP is regulated by the direct binding of Cdc42 ( refs 7, 8 ), which exposes the domain in N-WASP that activates the Arp2/3 complex
Rohatgi et al., J Biol Chem 2001 : The Wiskott-Aldrich syndrome protein (WASP) and its relative neural WASP (N-WASP) regulate the nucleation of actin filaments through their interaction with the Arp2/3 complex and are regulated in turn by binding to GTP bound Cdc42 and phosphatidylinositol 4,5-bisphosphate
Luna et al., Mol Biol Cell 2002 : We therefore conclude that Cdc42 regulates Golgi-to-ER protein transport in an N-WASP dependent manner
Sukumvanich et al., Cell Motil Cytoskeleton 2004 : The ability of CSA to detect only active N-WASP was demonstrated by in vitro experiments using immunoprecipitation of active N-WASP from EGF stimulated cells and Cdc42 activation of N-WASP activity
Tang et al., J Biol Chem 2005 : In tracheal smooth muscle, agonist induced actin polymerization is mediated by activation of neuronal Wiskott-Aldrich syndrome protein ( N-WASp ) and the Arp ( actin related protein ) 2/3 complex, and activation of the small GTPase Cdc42 regulates the activation of N-WASp ... These results suggest that CrkII plays a critical role in the regulation of N-WASp activation, perhaps by regulating the activation of Cdc42 , and that it thereby regulates actin polymerization and active tension generation in tracheal smooth muscle
Tomasevic et al., Biochemistry 2007 : Differential regulation of WASP and N-WASP by Cdc42 , Rac1, Nck, and PI ( 4,5 ) P2
Pommereit et al., J Cell Sci 2007 : An NGF induced Exo70-TC10 complex locally antagonises Cdc42 mediated activation of N-WASP to modulate neurite outgrowth ... Experiments involving siRNA mediated knockdown of Cdc42 and overexpression of constitutively active Cdc42 confirm that the Exo70-TC10 complex mainly targets the NGF induced Cdc42 dependent activation of N-WASP
Park et al., Mol Biol Cell 2009 : Cdc42 was necessary for WASP/N-WASP activation, as determined using a conformation-sensitive antibody against WASP/N-WASP and partial restoration of phagocytosis in Cdc42 reduced cells by expression of a constitutively activated WASP ... These results indicate that Cdc42 is essential for the activation of WASP and N-WASP , leading to actin assembly and phagocytic cup formation by macrophages during Fc ( gamma ) R-mediated phagocytosis
Kessels et al., Eur J Cell Biol 2011 : The underlying molecular mechanisms for Arp2/3 complex activation at the neuronal plasma membrane include a recruitment and an activation of N-WASP by lipid- and F-actin binding adaptor proteins, Cdc42 and phosphatidyl-inositol- ( 4,5 ) -bisphosphate ( PIP ( 2 ) )
Campellone et al., J Biol Chem 2012 (Escherichia coli Infections) : Under native conditions, this N-WASP/WIP complex is activated by the small GTPase Cdc42 in concert with several different SH3 ( Src-homology-3 ) domain containing proteins
Dehapiot et al., Dev Biol 2013 : Cdc42 inhibition in MII oocytes resulted in the release of N-WASP into the cytosol, a loss of the polarized F-actin cap, and a failure to protrude the second polar body