◀ Back to EIF4G1
EIF4G1 — EIF4G3
Pathways - manually collected, often from reviews:
-
BioCarta internal ribosome entry pathway:
eIF-4G/eIF3/EIF-4A complex (EIF4G1-EIF4G2-EIF4G3-EIF3S10-EIF4A1)
→
eIF-4G/eIF-4E/PTB complex (EIF4G1-EIF4G2-EIF4G3-EIF4E-PTBP1_PTBP2)
(translational initiation, activates)
-
BioCarta internal ribosome entry pathway:
eIF-4G/eIF-4E/PTB complex (EIF4G1-EIF4G2-EIF4G3-EIF4E-PTBP1_PTBP2)
→
eIF-4G/eIF3/EIF-4A complex (EIF4G1-EIF4G2-EIF4G3-EIF3S10-EIF4A1)
(modification, collaborate)
-
BioCarta internal ribosome entry pathway:
eIF-4G/eIF-4E/PTB complex (EIF4G1-EIF4G2-EIF4G3-EIF4E-PTBP1_PTBP2)
→
c-Myc mRNA/eIF-4G (dimer)/eIF-3/eIF-4E/eIF-4A/PTB complex (EIF4A1-EIF4E-EIF3S10-EIF4G1-EIF4G2-EIF4G3-PTBP1_PTBP2)
(modification, collaborate)
-
BioCarta internal ribosome entry pathway:
eIF-4G/eIF3/EIF-4A complex (EIF4G1-EIF4G2-EIF4G3-EIF3S10-EIF4A1)
→
c-Myc mRNA/eIF-4G (dimer)/eIF-3/eIF-4E/eIF-4A/PTB complex (EIF4A1-EIF4E-EIF3S10-EIF4G1-EIF4G2-EIF4G3-PTBP1_PTBP2)
(modification, collaborate)
-
BioCarta internal ribosome entry pathway:
Active caspases (CASP5/CASP3/CASP4/CASP8/CASP1/CASP7/CASP6/CASP2/CASP9/CASP10)
→
eIF-4G/eIF-4E/PTB complex (EIF4G1-EIF4G2-EIF4G3-EIF4E-PTBP1_PTBP2)
(modification, activates)
-
BioCarta internal ribosome entry pathway:
Active caspases (CASP5/CASP3/CASP4/CASP8/CASP1/CASP7/CASP6/CASP2/CASP9/CASP10)
→
eIF-4G/eIF3/EIF-4A complex (EIF4G1-EIF4G2-EIF4G3-EIF3S10-EIF4A1)
(modification, activates)
-
BioCarta internal ribosome entry pathway:
Active caspases (CASP5/CASP3/CASP4/CASP8/CASP1/CASP7/CASP6/CASP2/CASP9/CASP10)
→
c-Myc mRNA/eIF-4G (dimer)/eIF-3/eIF-4E/eIF-4A/PTB complex (EIF4A1-EIF4E-EIF3S10-EIF4G1-EIF4G2-EIF4G3-PTBP1_PTBP2)
(modification, activates)
-
BioCarta internal ribosome entry pathway:
eIF-4G/eIF-4E/PTB complex (EIF4G1-EIF4G2-EIF4G3-EIF4E-PTBP1_PTBP2)
→
eIF-4G/eIF3/EIF-4A complex (EIF4G1-EIF4G2-EIF4G3-EIF3S10-EIF4A1)
(modification, collaborate)
-
BioCarta internal ribosome entry pathway:
eIF-4G/eIF-4E/PTB complex (EIF4G1-EIF4G2-EIF4G3-EIF4E-PTBP1_PTBP2)
→
c-Myc mRNA/eIF-4G (dimer)/eIF-3/eIF-4E/eIF-4A/PTB complex (EIF4A1-EIF4E-EIF3S10-EIF4G1-EIF4G2-EIF4G3-PTBP1_PTBP2)
(modification, collaborate)
-
BioCarta internal ribosome entry pathway:
eIF-4G/eIF3/EIF-4A complex (EIF4G1-EIF4G2-EIF4G3-EIF3S10-EIF4A1)
→
c-Myc mRNA/eIF-4G (dimer)/eIF-3/eIF-4E/eIF-4A/PTB complex (EIF4A1-EIF4E-EIF3S10-EIF4G1-EIF4G2-EIF4G3-PTBP1_PTBP2)
(modification, collaborate)
-
BioCarta eukaryotic protein translation:
40s Ribosome subunit/RNA strand complex ()
→
48s Ribosome subunit complex (EIF3S10-EIF2S1-EIF2S2-EIF2S3-EIF4A1-EIF4A2-EIF4G1-EIF4G2-EIF4G3-EIF4E)
(modification, collaborate)
-
BioCarta eukaryotic protein translation:
eIF3 (EIF3S10)
→
48s Ribosome subunit complex (EIF3S10-EIF2S1-EIF2S2-EIF2S3-EIF4A1-EIF4A2-EIF4G1-EIF4G2-EIF4G3-EIF4E)
(modification, collaborate)
-
BioCarta eukaryotic protein translation:
eIF1 (EIF1)
→
48s Ribosome subunit complex (EIF3S10-EIF2S1-EIF2S2-EIF2S3-EIF4A1-EIF4A2-EIF4G1-EIF4G2-EIF4G3-EIF4E)
(modification, activates)
-
BioCarta eukaryotic protein translation:
48s Ribosome subunit complex (EIF3S10-EIF2S1-EIF2S2-EIF2S3-EIF4A1-EIF4A2-EIF4G1-EIF4G2-EIF4G3-EIF4E)
→
EIF-2 complex (EIF2S1-EIF2S2-EIF2S3)
(modification, collaborate)
-
BioCarta internal ribosome entry pathway:
PTB (PTBP1/PTBP2)
→
c-Myc mRNA/eIF-4G (dimer)/eIF-3/eIF-4E/eIF-4A/PTB complex (EIF4A1-EIF4E-EIF3S10-EIF4G1-EIF4G2-EIF4G3-PTBP1_PTBP2)
(modification, collaborate)
-
BioCarta internal ribosome entry pathway:
PTB (PTBP1/PTBP2)
→
eIF-4G (dimer) complex (EIF4G1-EIF4G2-EIF4G3)
(modification, collaborate)
-
BioCarta internal ribosome entry pathway:
eIF3 (EIF3S10)
→
c-Myc mRNA/eIF-4G (dimer)/eIF-3/eIF-4E/eIF-4A/PTB complex (EIF4A1-EIF4E-EIF3S10-EIF4G1-EIF4G2-EIF4G3-PTBP1_PTBP2)
(modification, collaborate)
-
BioCarta internal ribosome entry pathway:
eIF3 (EIF3S10)
→
eIF-4G (dimer) complex (EIF4G1-EIF4G2-EIF4G3)
(modification, collaborate)
-
BioCarta internal ribosome entry pathway:
c-Myc mRNA/eIF-4G (dimer)/eIF-3/eIF-4E/eIF-4A/PTB complex (EIF4A1-EIF4E-EIF3S10-EIF4G1-EIF4G2-EIF4G3-PTBP1_PTBP2)
→
EIF-4E (EIF4E)
(modification, collaborate)
-
BioCarta internal ribosome entry pathway:
c-Myc mRNA/eIF-4G (dimer)/eIF-3/eIF-4E/eIF-4A/PTB complex (EIF4A1-EIF4E-EIF3S10-EIF4G1-EIF4G2-EIF4G3-PTBP1_PTBP2)
→
EIF-4A (EIF4A1)
(modification, collaborate)
-
BioCarta internal ribosome entry pathway:
c-Myc mRNA/eIF-4G (dimer)/eIF-3/eIF-4E/eIF-4A/PTB complex (EIF4A1-EIF4E-EIF3S10-EIF4G1-EIF4G2-EIF4G3-PTBP1_PTBP2)
→
eIF-4G (dimer) complex (EIF4G1-EIF4G2-EIF4G3)
(modification, collaborate)
-
BioCarta internal ribosome entry pathway:
EIF-4E (EIF4E)
→
eIF-4G (dimer) complex (EIF4G1-EIF4G2-EIF4G3)
(modification, collaborate)
-
BioCarta internal ribosome entry pathway:
EIF-4A (EIF4A1)
→
eIF-4G (dimer) complex (EIF4G1-EIF4G2-EIF4G3)
(modification, collaborate)
-
BioCarta eukaryotic protein translation:
43s Ribosome subunit complex (EIF3S10-EIF2S1-EIF2S2-EIF2S3)
→
48s Ribosome subunit complex (EIF3S10-EIF2S1-EIF2S2-EIF2S3-EIF4A1-EIF4A2-EIF4G1-EIF4G2-EIF4G3-EIF4E)
(modification, collaborate)
-
BioCarta eukaryotic protein translation:
43s Ribosome subunit complex (EIF3S10-EIF2S1-EIF2S2-EIF2S3)
→
eIF4F/mRNA complex (EIF4A1-EIF4A2-EIF4G1-EIF4G2-EIF4G3-EIF4E)
(modification, collaborate)
-
BioCarta eukaryotic protein translation:
48s Ribosome subunit complex (EIF3S10-EIF2S1-EIF2S2-EIF2S3-EIF4A1-EIF4A2-EIF4G1-EIF4G2-EIF4G3-EIF4E)
→
eIF4F/mRNA complex (EIF4A1-EIF4A2-EIF4G1-EIF4G2-EIF4G3-EIF4E)
(modification, collaborate)
Protein-Protein interactions - manually collected from original source literature:
Studies that report less than 10 interactions are marked with *
Text-mined interactions from Literome
Wang et al., Am J Physiol Heart Circ Physiol 2000
:
Concomitant with this,
insulin increased the binding of eIF4E to
eIF4G
Korneeva et al., J Biol Chem 2000
:
Surprisingly, the binding of eIF3 and eIF4A to the central region was mutually cooperative ;
eIF3 binding to eIF4G increased 4-fold in the presence of eIF4A, and conversely, eIF4A binding to the central ( but not COOH-terminal ) region of
eIF4G increased 2.4-fold in the presence of eIF3 ... Surprisingly, the binding of eIF3 and eIF4A to the central region was mutually cooperative ; eIF3 binding to eIF4G increased 4-fold in the presence of eIF4A, and conversely, eIF4A binding to the central ( but not COOH-terminal ) region of
eIF4G increased 2.4-fold in the presence of
eIF3
Borman et al., Nucleic Acids Res 2000
:
Additionally, we show that the
eIF4G-PABP interaction on mRNAs which are capped and polyadenylated significantly
increases the affinity of
eIF4E for the 5 ' cap
Scheper et al., Mol Cell Biol 2001
:
Interestingly, we found that the association of both Mnk1 and Mnk2 with eIF4G increased upon inhibition of the MAPK pathways while activation of
ERK resulted in decreased binding to
eIF4G
Ali et al., J Virol 2001
:
In addition, the HAV IRES was inhibited by addition of
eIF4E binding protein 1 , which binds tightly to eIF4E and sequesters it, thus
preventing its association with
eIF4G ... This suggests two alternative models : ( i ) initiation requires a direct interaction between an internal site in the IRES and
eIF4E/4G , an interaction which involves the cap binding pocket of eIF4E in addition to any direct eIF4G-RNA interactions ; or ( ii ) it
requires eIF4G in a particular conformation which can be attained only if eIF4E is bound to it, with the cap binding pocket of the eIF4E unoccupied
Pyronnet et al., Genes Dev 2001
:
Cap dependent translation is
mediated by
eIF4F , a protein complex composed of three subunits as follows : eIF4E, which recognizes the mRNA 5 ' cap structure ; eIF4A, an RNA-helicase ; and
eIF4G , a scaffolding protein that binds eIF4E, eIF4A, and the eIF4E-kinase Mnk1 simultaneously
Morley et al., FEBS Lett 2001
:
Furthermore, MG132 also promotes the cleavage of
eIF4G and the
activation of
caspase-3-like activity in a caspase-8-deficient Jurkat cell line which is resistant to anti-Fas mediated apoptosis
Ling et al., Mol Cell Biol 2002
:
The translational function of
SLBP genetically
required eukaryotic initiation factor 4E (eIF4E),
eIF4G , and eIF3, and expressed SLBP coisolated with S. cerevisiae initiation factor complexes that bound the 5 ' cap in a manner dependent on eIF4G and eIF3
O'Connor et al., Am J Physiol Endocrinol Metab 2003
:
Both
insulin and amino acids increased the phosphorylation of ribosomal protein S6 kinase ( S6K1 ) and the eIF4E binding protein ( 4E-BP1 ), decreased the binding of 4E-BP1 to eIF4E,
increased eIF4E binding to
eIF4G , and increased fractional protein synthesis rates but did not affect eIF2B activity
Bush et al., Endocrinology 2003
(Body Weight) :
Feeding increased protein synthesis and translational efficiency in both muscle and liver of control and GH-treated pigs, and this was associated with increased 4E-BP1 and S6 kinase 1 phosphorylation, decreased association of eukaryotic initiation factor (eIF) 4E with 4E-BP1, and
increased association of
eIF4E with
eIF4G ... Feeding increased protein synthesis and translational efficiency in both muscle and liver of control and GH-treated pigs, and this was associated with increased 4E-BP1 and S6 kinase 1 phosphorylation, decreased association of eukaryotic initiation factor (eIF) 4E with
4E-BP1 , and
increased association of eIF4E with
eIF4G
He et al., Mol Cell Biol 2003
:
Interestingly, excess
eIF1 carrying the sui1-1 mutation, known to relax the accuracy of start site selection, did not inhibit the growth of the eIF4G mutant, and sui1-1
reduced the interaction between
eIF4G and eIF1 in vitro
Bolster et al., J Nutr 2004
:
However, binding of
eIF4E to eIF4G and eIF4G ( Ser-1108 ) phosphorylation in the eIF4E immunoprecipitate were
enhanced as was
eIF4G ( Ser-1108 ) phosphorylation in the total tissue extract after perfusion with medium containing 10X leucine
Orton et al., J Biol Chem 2004
:
Phosphorylation of
Mnk1 by caspase activated Pak2/gamma-PAK
inhibits phosphorylation and interaction of
eIF4G with Mnk ... Phosphorylation of Erk2 activated
Mnk1 by Pak2 has no effect on phosphorylation of eIF4E but
reduces phosphorylation of
eIF4G by Mnk1 by up to 50 %
Bassili et al., J Gen Virol 2004
:
Extra
eIF4F added to the translation reaction does not
restore full IRES activity or
eIF4G binding, indicating that disturbances in the structure of this conserved element can not be overcome by increased initiation factor concentrations
Tao et al., Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2004
(Laryngeal Neoplasms) :
The protein expression of eIF4E,
eIF4G , bFGF and VEGF were significantly inhibited by elemene ; and the mRNA expression of bFGF and
VEGF were
inhibited either ... The protein expression of eIF4E,
eIF4G , bFGF and VEGF were significantly inhibited by elemene ; and the mRNA expression of
bFGF and VEGF were
inhibited either
Korneeva et al., J Biol Chem 2005
:
eIF4A alone has only weak
ATPase and helicase activities, but these are
stimulated by
eIF4G , eIF4B, and eIF4H
Saelens et al., J Cell Biol 2005
(Necrosis) :
The rapid drop in protein synthesis observed in apoptosis correlates with caspase dependent breakdown of eukaryotic translation initiation factor (
eIF) 4G ,
activation of the double stranded RNA activated protein kinase
PKR , and phosphorylation of its substrate eIF2-alpha
Williamson et al., Am J Physiol Endocrinol Metab 2005
:
This appeared to be due to an attenuation of
insulin stimulated
eIF4E.eIF4G association, because other stimulatory effects of INS, e.g., phosphorylation of ERK1/2, 4E-BP1, S6K1, eIF4G, and eIF4E and eIF4E.4E-BP1 association, were unaffected
Greenberg et al., Oncogene 2005
(Breast Neoplasms) :
The hyperphosphorylation of 4E-BP1 by PTX
increased the association of
eIF-4E with
eIF-4G , whereas cotreatment with purvalanol A inhibited the association of eIF-4E with eIF-4G in PTX treated cells
Vary et al., Am J Physiol Regul Integr Comp Physiol 2006
(Sepsis) :
In addition, phosphorylation of
eIF4G and ribosomal protein S6 kinase-1 ( S6K1 ) was also
enhanced by
IGF-I
Walsh et al., Genes Dev 2006
:
While the translational repressor
4E-BP1 regulates binding of eIF4E to
eIF4G , the forces required to construct an eIF4F complex remain unidentified
Harris et al., EMBO J 2006
:
mTOR dependent stimulation of the association of
eIF4G and eIF3 by insulin ... Here, we present evidence that mTOR interacts directly with eIF3 and that
mTOR controls the association of eIF3 and
eIF4G ... This novel effect was blocked by rapamycin and other inhibitors of
mTOR , and it
required neither eIF4E binding to
eIF4G nor eIF3 binding to the 40S ribosomal subunit
García-Bonilla et al., Neurochem Res 2006
(Ischemic Attack, Transient...) :
Although IT did not promote significant changes in
p35 and p25 levels, it
induced a slight increase in calpastatin and
eIF4G levels in the hippocampal subregions after 4 h of reperfusion ... Although IT did not promote significant changes in p35 and
p25 levels, it
induced a slight increase in calpastatin and
eIF4G levels in the hippocampal subregions after 4 h of reperfusion
Renner et al., Carcinogenesis 2007
(Adenocarcinoma...) :
Additionally, we describe that the phosphorylation of AKT and
eIF4G , as well as the elevation of the Mst1 and RanBP2 protein levels, can be
inhibited in vivo in transgenic animals by the
PI3K inhibitor LY294002
Suryawan et al., Am J Physiol Endocrinol Metab 2007
:
Both
INS and AA decreased the binding of 4E-BP1 to eIF4E and
increased eIF4E binding to
eIF4G ; these effects were greater in 6-day-old than in 26-day-old pigs
Iresjö et al., Clin Sci (Lond) 2008
(Neoplasms) :
Overnight
TPN increased the formation of active
eIF4G-eIF4E ( where eIF is eukaryotic-initiation factor ) complexes ( P < 0.05 ), whereas the inhibitory complex 4E-BP1 ( eIF4E binding protein ) -eIF4E was moderately decreased ( P < 0.06 )
Constantinou et al., Biol Cell 2008
(Leukemia, Erythroblastic, Acute) :
Binding of eIF4E to
eIF4G is
inhibited in a competitive manner by
4E-BP1
Vary et al., Alcohol Clin Exp Res 2008
:
Furthermore, the data suggest that protein synthesis in rats fed a diet containing ethanol is limited by
mTOR dependent reduction in phosphorylation of S6K1 ( Thr ( 389 ) ) and
eIF4G ( Ser ( 1108 ) ) secondary to reduced phosphorylation of mTOR ( Ser ( 2448 ) )
Petegnief et al., Biochem J 2008
:
NOS inhibitors also
prevented NMDA induced
eIF4G degradation, 4E-BP1 dephosphorylation, decreased eIF4E-eIF4G binding and cell death
Svitkin et al., EMBO J 2009
:
These data strongly suggest that competition between YB-1 and
eIF4G for mRNA binding is
required for efficient stimulation of
eIF4F activity by PABP
Iwasaki et al., Mol Cell 2009
:
In contrast,
Ago2-RISC competitively
blocks the interaction of eIF4E with
eIF4G and inhibits the cap function ... In contrast,
Ago2-RISC competitively
blocks the interaction of eIF4E with
eIF4G and inhibits the cap function
Tuteja et al., Communicative & integrative biology 2009
:
eIF4E directly interacts with the cap structure, eIF4A is an RNA helicase and
eIF4G acts as a scaffold for the complex
Fan et al., Neoplasia (New York, N.Y.) 2010
(Carcinoma, Non-Small-Cell Lung...) :
As expected,
4EGI-1 inhibited
eIF4E/eIF4G interaction and reduced the levels of cyclin D1 and hypoxia inducing factor-1alpha ( HIF-1alpha ), both of which are regulated by a cap dependent translation mechanism ... However, inhibition of
eIF4E/eIF4G interaction by knockdown of eIF4E effectively
reduced the levels of
cyclin D1 and HIF-1alpha but failed to induce DR5 expression, downregulate c-FLIP levels, or augment TRAIL induced apoptosis ... However, inhibition of
eIF4E/eIF4G interaction by knockdown of eIF4E effectively
reduced the levels of cyclin D1 and
HIF-1alpha but failed to induce DR5 expression, downregulate c-FLIP levels, or augment TRAIL induced apoptosis
Jeske et al., EMBO J 2011
:
The initiation factor
eIF4G is specifically displaced, and
48S pre-initiation complex formation is
inhibited
Montpetit et al., Nature 2011
:
These findings explain how Gle1 ( InsP6 ), Nup159 and Dbp5 collaborate in mRNA export and provide a general mechanism for DEAD-box
ATPase regulation by
Gle1/eIF4G-like activators
Petersson et al., Biol Cell 2012
:
The p53 target gene
TRIM22 directly or indirectly interacts with the translation initiation factor eIF4E and
inhibits the binding of eIF4E to
eIF4G ... Here, we demonstrate that
TRIM22 directly or indirectly interacts with the eukaryotic translation initiation factor ( eIF)4E, and
inhibits the binding of eIF4E to
eIF4G , thus disturbing the assembly of the eIF4F complex, which is necessary for cap dependent translation
Ohlmann et al., EMBO J 1996
:
In contrast, the efficient translation of the second cistron of bicistronic mRNAs, directed by two distinct Internal Ribosome Entry Segments ( IRES ), exhibits no requirement for
eIF4E but is
dependent upon either intact
eIF4G or the Ct domain
Kimball et al., Am J Physiol 1997
:
In part, the increased binding of
eIF-4G to eIF-4E was a
result of release of
eIF-4E bound to the translational regulator, PHAS-I, through a mechanism involving enhanced phosphorylation of PHAS-I ... However, the insulin induced association of eIF-4E and
eIF-4G was not
due to increased net phosphorylation of
eIF-4E because insulin decreased the amount present in the phosphorylated form from 86 to 59 % of total eIF-4E ... However, the
insulin induced association of eIF-4E and
eIF-4G was not due to increased net phosphorylation of eIF-4E because insulin decreased the amount present in the phosphorylated form from 86 to 59 % of total eIF-4E
Haghighat et al., J Biol Chem 1997
:
eIF4G dramatically
enhances the binding of
eIF4E to the mRNA 5'-cap structure ... We demonstrate that the interaction of
eIF4E with the mRNA 5'-cap structure is dramatically
enhanced by
eIF4G , as determined by a UV-induced cross linking assay
Neumar et al., J Cereb Blood Flow Metab 1998
(Ischemic Attack, Transient) :
Because eIF-4G is a substrate of calpain, these studies were undertaken to examine whether
calpain I activation during global brain ischemia
contributes to the degradation of
eIF-4G in vivo
Marissen et al., Mol Cell Biol 1998
:
In addition, purified
caspase 3
caused cleavage of purified
eIF4G , demonstrating that eIF4G could serve as a substrate for caspase 3
Morley et al., FEBS Lett 1998
:
In this study, we show that activation of the
Fas/CD95 receptor complex in Jurkat cells
induces the degradation of
eIF4G , the inhibition of total protein synthesis and cell death ... Studies with the specific inhibitor, SB203580, have shown that signalling through the p38 MAP kinase pathway is not required for either the
Fas/CD95 induced cleavage of
eIF4G or cell death