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CD14 — IRF6

Text-mined interactions from Literome

Kent et al., J Periodontol 1999 (Periodontal Diseases) : In order to determine if LPS stimulation was mediated through the LPS receptor, CD14 , surface receptors on HGF were assessed by flow cytometry and the total RNA CD14 mRNA was assessed
Ohki et al., Immunol Cell Biol 1999 : No significant difference was observed in the expression of CD14 on WEHI-3 cells cultured in the presence and absence of serum, suggesting that CD14 is not involved in the serum mediated suppression of these LPS-responses
Fan et al., Infect Immun 1999 : LPS binding protein (LBP) is required to facilitate the binding of LPS to CD14 in vitro and is necessary for the induction of an inflammatory response to LPS in vivo
Tohme et al., Infect Immun 1999 : In conjunction with a serum protein, LPS binding protein (LBP), LPS-CD14 interactions mediate many LPS functions in the inflammatory response
Enomoto et al., Am J Physiol 1999 : In isolated Kupffer cells, LPS induced increases in intracellular Ca2+ concentration, tumor necrosis factor-alpha production, and CD14 were increased, as previously reported
Kimura et al., Scand J Immunol 2000 (Lymphoma, B-Cell) : In the absence of serum or with the addition of anti-CD14 monoclonal antibodies, however, LPS-stimulation induced neither the up-regulation of CD14 and TGF-beta mRNA nor an increase in IgA secretion
Imai et al., Infect Immun 2000 : In this study, we investigated whether TGF-beta1 inhibition of LPS induced expression of inflammatory cytokines in the septic shock results from downregulation of LPS stimulated expression of CD14 , an LPS receptor ... LPS stimulated expression of CD14 was dramatically inhibited by addition of antisense, but not sense, c-fos and c-jun oligonucleotides
Pedron et al., FEMS Immunol Med Microbiol 2000 : Lipopolysaccharide (LPS) of Gram negative bacteria interacts with a CD14 independent receptor of mouse bone marrow granulocytes ( BMC ), and triggers in these cells the expression of CD14 , an inducible type of LPS receptor ( iLpsR )
Becker et al., J Biol Chem 2000 : Thus, LPS induction of hBD2 in hTBE cells requires CD14 , which may complex with a TLR to ultimately activate NF-kappa B
Pedron et al., Immunology 2000 : We established in previous studies that the binding of Salmonella lipopolysaccharide (LPS) to constitutive receptors of low affinity triggers the expression of the inducible LPS binding molecule CD14 in bone marrow cells ( BMC ) of C3H/HeOU mice, but not in BMC from C3H/HeJ mice
Jiang et al., J Immunol 2000 : Using fluorescence resonance energy transfer (RET) techniques, we demonstrate that LPS triggers a physical association between CD14 and TLR4 ... Because LPS stimulation upregulates CD14 and TLR4 expression, it was necessary to control for the possibility that these newly expressed molecules were associated with one another independent of LPS stimulation
Kidd et al., Digestion 2000 (Helicobacter Infections...) : Our results demonstrated : ( 1 ) H. pylori LPS ( 10 ( -12 ) to 10 ( -7 ) M ) stimulated basal DNA synthesis ( 2.2-fold ) with an estimated EC ( 50 ) of 10 ( -10 ) M ; ( 2 ) this proliferative response correlated with an increase in ODC activity ( 1.4-fold, EC ( 50 ) approximately 10 ( -10 ) M ) which could be inhibited by a specific ODC inhibitor, difluoromethyl ornithine, at 10 ( -9 ) M ; ( 3 ) the CD14 receptor was identified in both naive and transformed ECL cells by reverse-transcription polymerase chain reaction, and ( 4 ) the effects of LPS were inhibited by blocking the CD14 receptor with its specific monoclonal antibody ( 1 : 100 )
Antal-Szalmás et al., Cytometry 2000 : Under conditions that ensure totally CD14 dependent LPS binding on human monocytes, the internalization mechanisms of LPS and CD14 were studied
Meerschaert et al., Am J Respir Cell Mol Biol 2000 : These results suggest that the survival enhancing effect of LPS on eosinophils requires the presence of CD14 ( + ) cells in the population
Lohrer et al., Endocrinology 2000 : Both TtT/GF cells and mouse pituitaries expressed CD14 , which binds the LPS/LBP complex, and Toll-like receptor type 4, which induces LPS signals
Pedron et al., Biochem Pharmacol 2000 : We have previously shown that stimulation of mouse bone marrow granulocytes ( BMC ) by lipopolysaccharide (LPS) induces the expression of CD14
Alexis et al., J Allergy Clin Immunol 2001 (Asthma...) : Significant associations were found between the LPS induced PMN response ( PMNs per milligram of sputum ) and both constitutive sCD14 ( R = 0.7, P =.005 ) and membrane bound CD14 ( R = 0.9, P =.01 )
Chakravortty et al., Infect Immun 2001 : Anti-CD14 antibody completely abrogated both LPS induced DNA synthesis and LPS induced phosphorylation of those MAP kinases, suggesting a critical role of membrane bound CD14 in LPS signaling
Pédron et al., Infect Immun 2001 : A phorbol ester induced a down-regulation of CD62L and blocked the LPS induced expression of CD14 ... Furthermore, BMG from L-selectin-deficient mice expressed normal levels of CD14 in response to LPS ... Taken together, these results demonstrate that in BMG, L-selectin is not the constitutive LPS receptor required for the LPS induced expression of CD14
Kitchens et al., J Endotoxin Res 2000 (Acute-Phase Reaction) : Soluble CD14 enhances LPS release from cells in the presence of lipoproteins, whereas LPS binding protein and phospholipid transfer protein do not
Nagaoka et al., J Immunol 2001 (Shock, Septic) : Interestingly, CAP18 and CAP11 possessed LPS binding activities, and they strongly suppressed the interaction of LPS with LPS binding protein that mediates the transport of LPS to CD14 to facilitate the activation of CD14 ( + ) cells by LPS
Alexis et al., J Allergy Clin Immunol 2001 (Asthma...) : We sought to determine whether blunting airway eosinophilic inflammation decreases CD14 expression and the subsequent airway polymorphonuclear neutrophil ( PMN ) response to inhaled LPS in subjects with atopic asthma ... A comparison of LPS induced inflammatory cells in sputum, CD14 expression, and methacholine responsiveness with FP or placebo was conducted
Rosas et al., J Mol Cell Cardiol 2001 (Sepsis) : Induction of myocardial NOS2 and CD14 ( not present in control ) by LPS/INF-gamma was approximately 2-3-fold greater in old compared to young animals
Neely et al., J Immunol 2001 : IL-15 protein was found stored intracellularly and stimulation of CD14 ( + ) monocytes with either LPS or GM-CSF resulted in mobilization of IL-15 stores to the plasma membrane
Martin et al., J Immunol 2001 : Thus, E. coli and P. gingivalis LPS differentially affect CD14 and TLR expression as well as secondary LPS associated responses
Brown et al., J Immunol 2001 : Treatment of cells with NaClO ( 3 ), an inhibitor of sulfation, prevented HA binding in a significant percentage of CD14 ( + ) PBMC induced by TNF-alpha, LPS , IL-1beta, or IFN-gamma
van Oosten et al., J Endotoxin Res 2001 : Although several binding sites have been described for LPS and LTA, only CD14 is involved in LPS signalling
Carracedo et al., Nephrol Dial Transplant 2002 (Uremia) : The effect of LPS , uraemia, and haemodialysis membrane exposure on CD14 expression in mononuclear cells and its relation to apoptosis ... This study also investigated whether LPS affects mononuclear cell CD14 expression and the apoptosis induced by uraemia and exposure to Cuprophan ( CU ) membrane ... Furthermore, LPS prevented the decrease in CD14 and reduced the rate of apoptosis
Gretzer et al., Biomaterials 2002 : LPS caused an upregulation of CD14 in adherent cells
Enomoto et al., Gastroenterology 2002 (Liver Diseases, Alcoholic) : Furthermore, thalidomide abolished the LPS induced increase in CD14 expression and [ Ca2+ ] i elevation in KCs
Dil et al., Vet Immunol Immunopathol 2002 : The objectives of the current study was to determine if the iNOS differences between K and GB2 macrophages are possibly due to differential expression of LPS induced TLR4, CD14 and/or nuclear factor kappa B (NF kappa B)
Latz et al., J Biol Chem 2002 : Mammalian responses to LPS require the expression of Toll-like receptor 4 (TLR4), CD14 , and MD-2
Fan et al., Shock 2002 (Bacteremia...) : Lipopolysaccharide (LPS) binding protein ( LBP ) greatly facilitates LPS activation of monocytic cells through the CD14 receptor, triggering activation of innate immune responses
Creery et al., Clin Diagn Lab Immunol 2002 : In contrast, dexamethasone inhibited IL-10 dependent LPS induced CD14 expression by interfering with IL-10 induced signals but not by blocking IL-10 production
Ruemmele et al., Gut 2002 (Inflammatory Bowel Diseases) : LPS signalling was independent of CD14 in HIEC, as these cells lack this receptor
Augusto et al., Infect Immun 2003 : ( iii ) LPS blocks the binding of radiolabeled CD14 to SP-C coated wells
Feng et al., Zhonghua Shao Shang Za Zhi 2002 : [ The role of LPS in the CD14 expression and the activation of Kupffer cells ] ... To explore the effect of LPS on the expression of CD14 and the activation of Kupffer cells (KCs)
Wang et al., J Immunol 2003 : SW480 and SW620 did not express membrane bound CD14 ; however, LPS in the presence of soluble CD14 ( sCD14 ) significantly up-regulated beta ( 1 ) integrin expression ; enhanced tumor cell attachment to fibronectin, collagen I, and laminin ; and strongly promoted tumor cell invasion through the Matrigel
Heine et al., Eur J Biochem 2003 (Chlamydia Infections) : Like other LPS, the chlamydial LPS used toll-like receptor TLR4 for signalling, but unlike other LPS activation was strictly CD14 dependent
Ogando et al., Reproduction 2003 (Bacterial Infections...) : LPS induced CD14 expression in endometrial glands, and this could have amplified the inflammatory response
Heagy et al., Shock 2003 (MAP Kinase Signaling System) : We sought to determine whether serum and/or CD14 are required for LPS to induce the endotoxin-tolerant state in human monocytes
Ivy et al., Biomed Sci Instrum 2003 : Macrophages express CD14 in response to lipopolysaccharides (LPS) extracted from bacterial coats
Vives-Pi et al., Clin Exp Immunol 2003 : The latter cells synthesized and secreted CD14 in response to lipopolysaccharide (LPS) in a time- and dose dependent manner
Wang et al., Vet Res 2003 : Soluble CD14 ( sCD14 ), in the circulation, binds to LPS and blocks LPS binding to mCD14 ... The role of bovine CD14 in cellular activation by LPS is undefined
Schuerwegh et al., Cytometry B Clin Cytom 2003 (Arthritis, Rheumatoid) : A two-color flow cytometric technique was used to measure interleukin (IL)-1beta, IL-6, tumor necrosis factor alpha (TNF-alpha), IL-10, and IL-12 production blocked intracellularly with brefeldin A in lipopolysaccharide (LPS) stimulated CD14 ( + ) monocytes and IL-2, IL-4, and IFN-gamma production in phorbol-12-mirystate-13-acetate ( PMA ) -stimulated CD3 ( + ) T lymphocytes in samples from patients with rheumatoid arthritis
Marchant et al., Eur J Immunol 1992 : Within 30 min LPS induced an increase in monocyte CD14 expression, peaking between 1 and 3 h and followed by a slow decrease ... Finally, LPS induced up-regulation of monocyte CD14 was associated with an increased binding of fluoresceinated LPS
Montrucchio et al., Thromb Haemost 2003 : They suggest that the activation of platelets by LPS is mainly dependent on leukocytes and especially monocytes as a result of CD14 and TLR4 engagement
Feng et al., Hepatobiliary Pancreat Dis Int 2003 : To assess the effect of lipopolysaccharides (LPS) on the expression of CD14 and TLR4 in rat Kupffer cells (KCs)
Kanaya et al., Eur J Immunol 2004 : Flow cytometry and real-time quantitative RT-PCR analysis revealed that Pg LPS , but not Ec LPS, preferentially up-regulated CD14 and CD16 expression at protein and mRNA levels ... Furthermore, Pg LPS preferentially induced the secretion of soluble CD14
Tsukamoto et al., Prostaglandins Other Lipid Mediat 2004 : The expression levels of the toll-like receptor 4 and CD14 , which were components of the LPS receptor complex, were increased by 9cRA in the presence and absence of LPS
Koshiba et al., Nucleosides Nucleotides Nucleic Acids 2004 (Inflammation) : Pro-inflammatory cytokine TNF-alpha ( TNF ) production from in vitro lipopolysaccharide (LPS) stimulated human peripheral blood CD14+ cells ( PB-CD14 ) was inhibited by A2A adenosine receptor ( AdoR) (A2AR ) or beta2 adrenergic receptor ( ADR ) ( beta2R ) signaling in a concentration dependent manner ... Furthermore A2AR agonist and beta2R agonist synergistically inhibited the TNF production of LPS stimulated PB-CD14 cells
Jeyaseelan et al., Infect Immun 2005 (Respiratory Distress Syndrome, Adult) : LPS binding and signaling involves CD14 and Toll-like receptor 4 (TLR4)
Köllisch et al., Immunology 2005 : In line with this, LPS induced mRNA expression of CD14 and TLR4 only in HaCaT cells
Olsson et al., Mol Immunol 2006 : LPS signaling is initiated by binding to Toll-like receptor-4 (TLR4) and the co-receptor CD-14 , leading to activation of downstream targets, such as MAP kinases ... In this study, we show that LPS causes translocation of CD-14 and MAP kinases ( ERK-2 and p38 ) to lipid rafts in the macrophage cell line RAW 264.7
Rahimi et al., J Immunol 2005 : Our results show that LPS induced CD14 expression on monocytic cells may be mediated by endogenously produced IL-10
Braedel-Ruoff et al., J Endotoxin Res 2005 : Our data show that not only purified LPS from L. pneumophila but also the microorganism itself stimulate BMDCs via TLR2 and that this stimulation is dependent on CD14 in this mouse model
Shuto et al., Biochem Biophys Res Commun 2005 : Membrane anchored CD14 is required for LPS induced TLR4 endocytosis in TLR4/MD-2/CD14 overexpressing CHO cells
Wang et al., Cell Mol Immunol 2004 : To investigate the effects of lanthanum chloride on binding of LPS to monocyte and CD 14 expression upregulation induced by LPS , human monocytes were analyzed by flow cytometry ( FCM )
Sümegi et al., Clin Immunol 2005 (Lupus Erythematosus, Systemic) : In order to study the possible action of glucocorticosteroids (GCS) on the CD14/Toll like receptor mediated activation of monocytes the CD14-expression, CD14 mediated LPS binding and activation of these cells of patients suffering from Systemic Lupus Erythematosus receiving no, low dose or pulse steroid treatment was studied ... Our results showed that the CD14-expression, CD14 dependent LPS binding and activation were significantly inhibited by the in vivo applied pulse steroid therapy
Møller et al., Cytokine 2005 : E. coli-LPS and LTA induced a dose dependent increase of CD14 , TLR2 and TLR4 expression on monocytes in whole blood
Liu et al., Clin Exp Pharmacol Physiol 2006 (Hepatitis, Autoimmune) : TNF-alpha, IL-6, LBP and CD14 mRNA expression in mouse liver may be involved in BCG plus LPS induced liver injury
Hayashi et al., Journal of thrombosis and haemostasis : JTH 2006 : LPS increased CD14 in hepatocytes and decreased it in SECs, but did not affect toll-like receptor-4 (TLR-4) expression in both cells
Maezawa et al., Journal of neuroinflammation 2006 : Previously, we have shown that specific stimulation of CD14/TLR-4 with lipopolysaccharide (LPS) leads to greatest innate immune response by primary microglial cultures from targeted replacement ( TR ) APOE4 mice and greatest p38MAPK dependent paracrine damage to neurons in mixed primary cultures and hippocampal slice cultures derived from TR APOE4 mice
Botero et al., J Endod 2006 : CD14 and TLR4 are responsible for LPS signaling in macrophages, but their expression levels and function in dental pulp cells are unknown
Venet et al., J Immunol 2006 (Shock, Septic) : We demonstrated that human Treg inhibit LPS induced retention of monocyte CD14
Sanz et al., Dev Comp Immunol 2007 : Finally, we report that LPS stimulation significantly up-regulated CD14 gene expression in porcine alveolar macrophages
Landmann et al., J Cell Biochem 1991 : LPS strongly enhanced CD14 in 45 h cultures of purified monocytes
Poulaki et al., FASEB J 2007 (Leukostasis...) : Although 17-AAG treatment did not reduce the LPS induced increase in total CD14 levels in leukocytes, it significantly decreased membrane CD14 levels
Nareika et al., J Endocrinol 2008 : After being chronically exposed to normal or high glucose for 2 weeks or longer, cells were treated with LPS for 24 h. Real-time PCR showed that although high glucose by itself did not increase CD14 expression significantly, it augmented LPS stimulated CD14 expression by 15-fold ... Further investigations using transcription factor activity assays and gel shift assays revealed that high glucose augmented LPS stimulated CD14 expression by enhancing transcription factor nuclear factor kappaB (NFkappaB) and activator protein-1 (AP-1) activities ... Taken together, this study has demonstrated a robust augmentation by high glucose of LPS stimulated CD14 expression through AP-1 and NFkappaB transcriptional activity enhancement, elucidating a new mechanism by which hyperglycemia boosts LPS elicited gene expression involved in inflammation and tissue destruction
Skrzeczyńska-Moncznik et al., Scand J Immunol 2008 : As expected, lipopolysaccharide (LPS) stimulated CD14 ( dim ) CD16 ( + ) MO produced TNF-alpha but little IL-10
Jawan et al., Toxicol Appl Pharmacol 2008 : The results of semi-quantitative RT-PCR demonstrated that PPF pretreatment significantly suppressed the LPS induced toll-like receptor (TLR)-4, CD14 , tumor necrosis factor (TNF)-alpha, and granulocyte-macrophage colony stimulating factor ( GM-CSF ) gene expression
Yin et al., Arch Pharm Res 2008 (Liver Diseases, Alcoholic) : LPS or ethanol induced the expression of CD14 , iNOS, and SCD1 and decreased RXR-alpha, which was completely reversed by tanshinone IIA
Kanaya et al., J Periodontal Res 2009 : Flow cytometry revealed that Pg fimbriae and peptidoglycan but not Ec LPS induced CD14 and CD16 expression on peripheral blood DCs ( CD14 ( - ) CD16 ( - ) )
Galbois et al., Hepatology 2009 (Inflammation...) : LPS induced TNF-alpha production and CD14 expression were significantly more marked in cirrhotic monocytes than in control monocytes, and both decreased significantly after rHDL incubation
D'Antò et al., Biomaterials 2009 : CD14 expression was up-regulated by 0.1 microg/ml LPS by about 10-fold after 24h and 100-fold after 48 h
Eckhardt et al., Biomaterials 2009 : The LPS induced expression of CD14 was inhibited by high TEGDMA concentrations
Sánchez-Lemus et al., Am J Physiol Regul Integr Comp Physiol 2009 (Disease Models, Animal) : The ARB reduced the LPS induced upregulation of CD14 gene expression ; expression of TNF-alpha and IL-6 mRNA and protein ; expression of IL-1beta and IkappaB-alpha mRNA ; COX-2 mRNA and protein expression and PGE ( 2 ) concentration ; inducible nitric oxide synthase (iNOS) gene and protein expression and iNOS activity ; and Nox2 gene expression and 8-isoprostane levels
Yin et al., J Neurosci Res 2009 (Alzheimer Disease...) : Soluble CD14 inhibited lipopolysaccharide (LPS)- or LPS/interferon-gamma induced nitric oxide production and cell death of microglia and astrocytes
Ma et al., Acta Anaesthesiol Scand 2010 : LPS stimulation resulted in an increased CD14 and TLR4 expression and increased TNF-alpha production in ATII cells
Wang et al., Acta Pharmacol Sin 2009 (Inflammation) : The mRNA expression of LPS receptor Toll-like receptor 4 (TLR4) and myeloid differentiation protein-2 ( MD-2 ) was not changed, while LPS induced CD14 expression was slightly reduced after SM905 treatment
Thorgersen et al., FASEB J 2010 (Sepsis) : Anti-CD14 did not affect LPS or E. coli DNA levels
Wang et al., Acta Pharmacol Sin 2009 (Inflammation) : Fenoterol, a beta(2)-adrenoceptor agonist, inhibits LPS induced membrane bound CD14 , TLR4/CD14 complex, and inflammatory cytokines production through beta-arrestin-2 in THP-1 cell line ... LPS induced membrane bound CD14 , TLR4/CD14 complex levels and elevation of inflammatory cytokines were all significantly reduced by pre-incubation of fenoterol ( P < 0.05 )
Sebai et al., Arch Biochem Biophys 2010 : LPS increased CD14 expression ; IRAK1 and a phosphorylated form of p38 MAPK protein
Hwang et al., Int Immunopharmacol 2010 : However, the LPS stimulated expression of LPS receptors, such as Toll-like receptor 4, myeloid differentiation factor-2, and CD14 , was unchanged after DSHP-U treatment at significantly high levels
Lubos et al., FASEB J 2010 : CD14 contributes to LPS signaling in leukocytes through formation of toll-like receptor 4/CD14 receptor complexes ; however, a specific role for endogenous cell-surface CD14 in endothelial cells is unclear ... Following LPS treatment, GPx-1 deficiency augmented LPS induced intracellular reactive oxygen species accumulation, CD14 expression, and intercellular adhesion molecule-1 ( ICAM-1 ) mRNA and protein expression compared to LPS treated control cells
Anas et al., PloS one 2010 (Acute Disease...) : Recently, in vitro studies revealed that CD14 is required for activation of the myeloid differentiation factor (MyD)88 dependent Toll-like receptor (TLR)4 signaling pathway by smooth ( S ) -LPS , but not by rough ( R ) -LPS ... At low doses, S-LPS and R-LPS induced neutrophil influx in a CD14 dependent manner
Piazza et al., Biochem Pharmacol 2010 : This compound inhibited LBP/CD14 dependent LPS transfer to MD-2.TLR4, resulting in reduced formation of a ( LPS-MD-2-TLR4 ) ( 2 ) complex
Holmlund et al., Clin Exp Immunol 2010 (Bacterial Infections...) : Breast milk in general abrogated the LPS induced down-regulation of surface CD14 and Toll-like receptor (TLR)-4 expression on CB monocytes, while inhibiting the PGN induced TLR-2 up-regulation
Jia et al., Shanghai Kou Qiang Yi Xue 2010 : To investigate the effect of lipopolysaccharides(LPS) extracted from Porphyromonas endodontalis ( P.e ) on the expression of CD14 in osteoblast ... P.e-LPS can induce the expression of CD14 in osteoblast MC3T3-E1, which indicates that P.e-LPS may play an important role in osteoblast through CD14
Bosshart et al., J Immunol Methods 2011 : Here, an experiment is presented to dissect the differences between LPS effects and cell culture effects on monocytic CD14 cell surface expression ex vivo
Thorley et al., PloS one 2011 : Primary ATII cells and alveolar macrophages and an immortalised ATI cell line ( TT1 ) elicited CD14 and MD2 dependent responses to LPS which did not require the addition of exogenous soluble CD14
Azor et al., Clin Exp Immunol 2011 (Chronic Disease...) : Furthermore, statin did not affect the tumour necrosis factor (TNF)-a secretion by lipopolysaccharide (LPS) stimulated PBMC or CD14+ cells in CIU patients
Ehrentraut et al., Shock 2011 (Endotoxemia...) : In addition, LPS increased the expression of CD14 and TLR2 in cardiac and aortic tissue
Jia et al., Zhonghua Kou Qiang Yi Xue Za Zhi 2011 : Pe-LPS can induce the expression of IL-6 in osteoblast MC3T3-E1 through CD-14 and TLR-4, but not TLR-2
Shimizu et al., Toxicol In Vitro 2012 (Bacterial Infections...) : LPS inhibited the expression of TLR4, CD14 , MD2 and NOD1 genes in FSH treated granulosa cells from small follicles
Rallabhandi et al., mBio 2012 (Respiratory Syncytial Virus Infections) : Toll-like receptor 4 (TLR4), a signaling receptor for structurally diverse microbe associated molecular patterns, is activated by the RSV fusion ( F ) protein and by bacterial lipopolysaccharide (LPS) in a CD14 dependent manner
Trøseid et al., Innate Immun 2013 : HIV infected patients had lower plasma LPS levels than IBD patients, but higher levels of soluble CD14 and Myeloid Differentiation ( MD ) 2, which interacts with TLR4 to initiate LPS signalling
Izban et al., PloS one 2012 : In 1,25-D3 pretreated cells, LPS differentially regulated protein expression - CD14 ( 21-fold increase ) and CD11b ( a transient 2-fold decrease ) - principally at the posttranscriptional level
Parajuli et al., Journal of neuroinflammation 2012 : These results suggest that GM-CSF upregulates TLR4 and CD14 expression in microglia through ERK1/2 and p38, respectively, and thus promotes the LPS receptor mediated inflammation in the CNS
Shao et al., PloS one 2013 (Keratitis...) : Lumican is an extracellular protein that associates with CD14 on the surface of macrophages and neutrophils, and promotes CD14-TLR4 mediated response to bacterial lipopolysaccharides (LPS)
Pedraza-Sánchez et al., Clinical and vaccine immunology : CVI 2013 : Thus, a reduced CD14 ( + ) CD16 ( + ) activated/differentiated monocyte subset and a correspondingly lower level of functional TLR4 on monocytes contributes to the relatively low TNF-a response to LPS observed in immunologically naive newborns compared to the response in adults
Kusunoki et al., J Exp Med 1995 : LPS binds to CD14, and the resulting LPS-CD14 complex induces synthesis of cytokines and up-regulation of adhesion molecules in a variety of cell types
Han et al., J Biol Chem 1994 : Additionally, NH-LBP inhibited LPS binding to LBP, inhibited the LBP promoted binding of LPS to CD14 , and inhibited the LBP dependent activation of rabbit peritoneal exudate macrophages
Gallay et al., Proc Natl Acad Sci U S A 1994 (Shock, Septic) : In vitro, we demonstrated that they interfere with LPS binding to monocytes or polymorphonuclear cells in different ways : by the mere prevention of binding of LPS to LBP thus preventing the binding of LPS to CD14 , or by reacting with LPS-LBP complexes thus mediating their binding to complement or Fc receptors on monocytes and on polymorphonuclear cells
Quentmeier et al., Leukemia 1994 (Leukemia, Monocytic, Acute) : IL-4 inhibits the LPS induced expression of CD14 and monocyte-specific esterase mRNA in MONO-MAC-6 cells
Eggesbø et al., Cytokine 1994 : We have examined basal and lipopolysaccharide (LPS) induced release of interleukin 1 beta (IL-1 beta), interleukin 6 (IL-6), interleukin 8 (IL-8), tumour necrosis factor-alpha (TNF-alpha) and soluble CD14 ( sCD14 ) in whole blood and peripheral blood mononuclear cells ( PBMC ) from 20 persons with either high ( 1.62-2.47 mmol/L ) or low ( 0.43-1.29 mmol/L ) levels of high-density lipoprotein ( HDL )
Loppnow et al., Infect Immun 1995 : Since the vascular SMC did not express endogenous CD14, we investigated the role of human serum derived soluble CD14 ( sCD14 ) for activation of SMC by LPS ... These results show that specific activation of vascular SMC by LPS does not involve binding to endogenous membrane CD14 , but that the activation of vascular SMC by LPS is mediated to a great extent by serum derived sCD14
Gegner et al., J Biol Chem 1995 : Under physiological conditions, lipopolysaccharide (LPS) activation of cells involves the LPS binding protein (LBP) and either membrane or soluble CD14
Kielian et al., J Leukoc Biol 1995 : Because LPS has varying effects on CD14 expression in vitro, we evaluated CD14 expression in response to LPS with a fully differentiated macrophage phenotype, the alveolar macrophage ... In addition, LPS induced CD14-like up-regulation correlated with secretion of tumor necrosis factor-alpha, regardless of serum concentration ... Therefore, these data suggest that LPS activation of porcine alveolar macrophages for 24 h increased CD14-like receptor expression
Huang et al., Shock 1994 : These studies demonstrate that rBPI23 acts as a specific and potent inhibitor of soluble CD14 mediated LPS induction
Otterlei et al., Scand J Immunol 1995 : Effects of anti-CD18 and LPS on CD14 expression on human monocytes ... These results indicate that CD18 participates in LPS induced TNF-alpha production as well as in regulating CD14 expression on monocytes
Schletter et al., Infect Immun 1995 : Binding of lipopolysaccharide (LPS) to an 80-kilodalton membrane protein of human cells is mediated by soluble CD14 and LPS binding protein
Parsons et al., Am J Respir Cell Mol Biol 1995 (Respiratory Distress Syndrome, Adult) : Neutrophils from normal subjects both upregulate CD14 in response to LPS and shed CD14 over time, suggesting that in patients CD14 receptors could have been previously upregulated and shed
Arditi et al., J Immunol 1995 : These findings demonstrate for the first time that increased protein tyrosine phosphorylation and activation of mitogen activated protein kinases occur rapidly after LPS stimulation of EC in the presence of soluble CD14
Yu et al., J Inflamm 1995 : LPS dependent interaction of Mac-2 binding protein with immobilized CD14 ... Binding of both LBP and Mac-2-BP to CD14 required the simultaneous presence of LPS
Pugin et al., Proc Natl Acad Sci U S A 1993 : Myeloid cell activation by lipopolysaccharides (LPS) involves two proteins, plasma LPS binding protein (LBP) and cell-membrane CD14 ... In this work we show that soluble CD14 ( sCD14 ) is required for activation of endothelial and epithelial cells by LPS
Gallay et al., J Immunol 1993 : Polymyxin B, an inhibitor of LPS mediated activation, essentially abrogated the LPS binding protein- and CD14 dependent binding of LPS to monocytes
Luchi et al., J Immunol 1993 : Serum thus augments CD14 mediated LPS binding, although the total amount of cell associated LPS increases only by a factor of two, on average, when serum is added ... In the absence of serum, LPS internalization occurs in the presence of a mAb that blocks LPS-CD14 binding, suggesting that an interaction with CD14 is not essential for LPS to traffic beyond the plasma membrane
Kirkland et al., J Biol Chem 1993 : At the concentrations studied ( 20-100 nM ) LPS binding required the expression of CD14 and could be inhibited by a subset of anti-CD14 monoclonal antibodies
Joseph et al., J Biol Chem 1994 (Second Messenger Systems) : This effect of LPS was markedly enhanced by LPS binding protein and required the LPS receptor CD14
Pugin et al., Prog Clin Biol Res 1995 (Gram-Negative Bacterial Infections...) : Thus, the whole blood effect begins with LPS activation of monocytes via cell membrane CD14 and results in endothelial cell activation by the effects of TNF and IL-1
Takamatsu et al., J Immunol 1996 : Granulocyte/macrophage CSF (GM-CSF) or IL-3 strongly enhanced LPS induced histamine formation and expression of CD14 on bone marrow derived macrophages, without affecting the expression of Mac-1 Ag
Nockher et al., Kidney Int 1995 : The release of soluble CD14 by cultured peripheral blood monocytes significantly increased in the presence of 0.01 ng/ml LPS during a five-day incubation experiment
Hopkins et al., Am J Physiol 1995 : Inhibitors of protein kinase C and protein tyrosine kinase prevented the upregulation of CD14 by LPS but did not effect the upregulation of CD14 by FBS
Horadagoda et al., Vet Immunol Immunopathol 1995 (Acute-Phase Reaction) : Bovine LBP enhanced the binding of LPS to human monocytes while enzymatic removal of the CD14 receptor abrogated this interaction
Galea et al., J Neuroimmunol 1996 : Here we show that lipopolysaccharide (LPS) induction of glial inducible nitric oxide synthase (iNOS) requires membrane ( m ) and soluble ( s ) forms of CD14
Landmann et al., Infect Immun 1996 : It is unknown how LPS regulates its own receptor CD14 in vitro ... Therefore, we investigated the effects of LPS on CD14 mRNA and membrane and soluble CD14 ( mCD14 and sCD14, respectively ) in human monocytes and macrophages
Landmann et al., J Infect Dis 1996 (Hemoglobinuria, Paroxysmal...) : Soluble CD14 ( sCD14 ) mediates lipopolysaccharide (LPS) activation of epithelial cells in vitro and may thereby be harmful in sepsis
Diamond et al., Proc Natl Acad Sci U S A 1996 : Together the data support that CD14 of epithelial cell origin mediates the LPS induction of an antibiotic peptide gene in TECs, providing evidence for the active participation of epithelial cells in the host 's local defense response to bacteria
Ikewaki et al., J Leukoc Biol 1996 : The antigen defined by mNI-11 was found to be expressed on U937 cells, LPS stimulated U937 cells, normal CD14+ cells ( monocytes/macrophages ), and human umbilical vein endothelial cells ( HUVECs )
Paape et al., Am J Vet Res 1996 : To examine Escherichia coli lipopolysaccharide (LPS) effects on expression of CD14 and CD18 cell surface receptors and lectin/carbohydrate mediated nonopsonic phagocytosis of E coli
Bellezzo et al., Am J Physiol 1996 : Since the role of CD14 in LPS signaling has not been evaluated in Kupffer cells, the resident hepatic macrophage, the purpose of this study was to characterize LPS mediated NF-kappa B activation under CD14 dependent ( 1 % serum, as a source of LBP ) and CD14 independent ( serum-free ) conditions
Becher et al., J Neurosci Res 1996 : Surface CD14 expression can be down-regulated by interaction with its ligand lipopolysaccharide (LPS) , and by the T-helper ( Th1 ) cytokine interferon-gamma (IFN-gamma) or the Th2 cytokine interleukin-4 (IL-4)
Takakuwa et al., Eur J Immunol 1996 : LPS enhanced CD14 expression only in Lpsn M phi, while all bacteria, or dsRNA, enhanced CD14 in Lpsn and Lpsd M phi ... However, LPS , bacteria and dsRNA induced CD14 in both TNFR2-/- and TNFR1-/- mice to a similar extent, revealing that this induction does not require TNF signaling
Nockher et al., J Immunol 1997 : In cultured monocytes, the LPS induced increase of membrane and soluble CD14 was markedly but not completely inhibited by prednisolone
Quentmeier et al., Immunology 1996 : LPS up-regulated expression of monocyte-specific esterase (MSE) mRNA as well as CD14 protein in MONO-MAC-6 cells ; both effects were inhibited by IL-4
Jungi et al., Immunol Lett 1996 : Both serum dependent and serum independent activation of M phi by LPS require cellular CD14 , as evidence by blocking studies with CD14-specific antibodies
Troelstra et al., Infect Immun 1997 : CD14 dependent association of LPS with monocytes was shown to be totally saturable
Hasday et al., Am J Physiol 1997 : Soluble CD14 ( sCD14 ) may block LPS stimulation of CD14 bearing cells while supporting LPS presentation to non-CD14 bearing cells
Hojman et al., J Surg Res 1997 : LPS down-regulated CD14 expression on monocytes in vivo and LPS also blunted the ability of monocytes to respond to other stimuli
Cauwels et al., Infect Immun 1997 : For the cultured monocytes, anti-CD14 inhibited induction of the inflammatory cytokines by the cell wall and LPS but not by intact pneumococcal bacteria
Miyata et al., Infect Immun 1997 (Bone Resorption) : In an additional experiment, we observed that chloramphenicol clearly inhibited P-LPS stimulated expression of the CD14 , IL-1beta, and IL-6 genes in calvarial cells
Jungi et al., Infect Immun 1997 : The role of serum factors such as lipopolysaccharide (LPS) binding protein ( LBP ) and of macrophage expressed CD14 and beta2 integrins in the activation of bovine macrophages by LPS was investigated
Heidenreich et al., J Immunol 1997 : The effects of LPS and IL-4 on the expression of CD14 , indicated by binding of Leu M3 Ab, and PCD of monocytes were studied simultaneously and in a kinetic fashion by multiparameter flow cytometry ... The present study shows that the LPS induced increase in CD14 expression rescued monocytes from apoptosis, whereas IL-4 treatment first down-regulated CD14 expression and consecutively evoked apoptosis
Amano et al., J Cell Physiol 1997 (Bone Resorption) : In the present study, we show, by using mouse embryonic calvarial cells, that endogenous CD14 and interleukin-1 beta (IL-1 beta) play an important role in the LPS mediated bone resorption and that interferon-gamma (IFN-gamma) functions as a strong inhibitor of this resorption by suppressing LPS stimulated expression of CD14 and IL-1 beta genes in the calvarial cells ... In addition, because LPS stimulated CD14 gene expression in the calvarial cells, these observations demonstrate the precise role of endogenous CD14 in LPS stimulated differentiation of osteoclastic cells and bone resorption
Schiff et al., J Leukoc Biol 1997 : 18E12 ( an anti-CD14 mAb that does not block LPS binding to CD14 but does inhibit CD14 dependent cell activation ) did not inhibit phagocytosis of LBP-opEc by THP1-wtCD14 cells
Schumann et al., Glia 1998 (Astrocytoma) : Both, LPS- and PCW induced responses of astrocytic cells required the presence of soluble CD14 ( sCD14 ) present in serum
Haziot et al., J Immunol 1998 : Since LPS induces high levels of these cytokines after its interaction with CD14, a protein expressed on the surface of monocytes and neutrophils, it has been assumed that CD14 mediates the LPS induction of APP expression
Fearns et al., Shock 1998 : Pretreatment with a combination of anti-lL-1beta and anti-tumor necrosis factor (TNF) antibodies was more effective in reducing LPS mediated induction of plasma CD14 and CD14 mRNA in liver than pretreatment with either antibody alone ... These studies demonstrate that regulation of CD14 gene expression by LPS in vivo involves multiple signals but is mediated, in part, by the cytokines IL-1beta and TNF-alpha
Mattern et al., J Immunol 1998 : We found that the accessory cell activity of monocytes during LPS induced T cell proliferation is characterized by the following features : LPS primed monocytes are competent stimulators of T cell proliferation ; interaction of LPS with monocytes during the priming step is dependent on CD14 and is sensitive to ammonia ; monocyte/T cell interactions are not MHC restricted but are strongly dependent on interactions of CD28 and/or CTLA-4 on T cells and their ligands CD80 and/or CD86 on monocytes
Takeshita et al., Inflamm Res 1998 : LPS alone showed only slight binding to neutrophils, but pretreatment with IFN-gamma or TNF-alpha before LPS exposure markedly increased LPS binding and CD14 expression on the surfaces of neutrophils
Stelter et al., Eur J Clin Invest 1998 (Disease Models, Animal...) : In vitro the soluble form of the endotoxin receptor CD14 ( sCD14 ) competitively prevents binding of LPS to membrane bound CD14 and inhibits LPS stimulated macrophage responses
Medvedev et al., J Immunol 1998 : LPS and GBS caused TNF secretion from human monocytes in a CD14 dependent manner, and soluble CD14 , LPS binding protein, or their combination potentiated both LPS- and GBS induced activities ... Thus, LPS and GBS differentially involve CD14 and CR3 or CR4 for signaling NF-kappaB activation in CHO cells and TNF release in human monocytes, and engage a different set of receptors and/or intracellular signaling pathways in mouse macrophages
Tapping et al., Prog Clin Biol Res 1998 : Roles for LBP and CD14 in the LPS dependent activation of a wide variety of cells have been established
Kirikae et al., Antimicrob Agents Chemother 1998 : The macrophage activation by CAZ released endotoxin as well as LPS was mainly dependent on the presence of serum factor and CD14 antigen
Jin et al., J Infect Dis 1998 : Since soluble CD14-lipopolysaccharide (LPS) complexes activate CD14 negative endothelial and epithelial cells, this study tested whether soluble CD14-PGN complexes activate human umbilical vein endothelial cells and epithelial-like U373 cells to secrete interleukin (IL)-6, express vascular cellular adhesion molecule-1, and translocate nuclear factor-kappaB
Sugawara et al., Infect Immun 1998 : These results suggest that ( i ) HGF consist of populations that are heterogeneous on the basis of different levels of expression of CD14 and ( ii ) CD14 ( high ) HGF secrete inflammatory cytokines in response to LPS via CD14
Wang et al., Arch Oral Biol 1998 : Engagement of LPS initiated the protein tyrosine phosphorylation of several intracellular proteins including extracellular signal regulated kinase ( ERK ) 1 and 2, and these events were suppressed by the anti-CD14 monoclonal
Chu et al., J Surg Res 1998 : To determine if these cytokines depressed LPS recognition resulting in such inhibition, we employed an anti-CD14 mAb ( UCHM-1 ; Sigma Chemical ) to address the role of CD14 in LPS transmembrane signaling
Haugen et al., Inflammation 1998 (Inflammation) : LPS stimulation had a downregulating effect on AM CD14 level, but after several hours of continuing decreased expression, an increased ( P < 0.05 ) CD14 expression was demonstrated, indicating de novo synthesis
Lacroix et al., Brain Pathol 1998 : At 6 h post-challenge, small positive cells were found throughout the entire parenchymal brain and dual labeling procedure indicated that different cells of myeloid origin have the ability to express CD14 in response to systemic LPS
Rovers et al., Atherosclerosis 1998 (Hyperlipoproteinemia Type II) : LPS induced cytokine production and expression of beta2-integrins and CD14 by peripheral blood mononuclear cells of patients with homozygous familial hypercholesterolemia
Comstock et al., J Mol Cell Cardiol 1998 (Necrosis) : LPS induced TNF-alpha release from and apoptosis in rat cardiomyocytes : obligatory role for CD14 in mediating the LPS response