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DIO1 — TNF
Text-mined interactions from Literome
Tang et al., Endocrinology 1995
:
In the present study, we used FRTL5 cells, a cultured rat thyroid follicular cell line, to examine the
effects of IFN-gamma and
TNF-alpha on
type I 5'-deiodinase ( 5'D-I ) activity and 5'D-I, thyroid peroxidase (TPO) and thyroglobulin (Tg) gene expression ... In TSH deprived FRTL-5 cells,
TNF-alpha and IFN-gamma
resulted in a small but dose dependent decrease in
5'D-I activity ...
TNF-alpha or IFN-gamma
blocked the TSH- or cAMP induced rise in
5'D-I activity ... 100 ng/ml
TNF-alpha and 100 U/ml IFN-gamma completely
blocked the TSH- or cAMP induced rise in
5' D-I activity ... Northern blot analyses were performed to examine the
effect of
TNF-alpha and IFN-gamma on
5'D-I gene expression
Ongphiphadhanakul et al., Eur J Endocrinol 1994
:
We have investigated the
effects of
TNF-alpha on
5'D-I in FRTL-5 cells, a cultured rat thyroid follicular cell line ...
Tumor necrosis factor-alpha did not significantly affect basal 5'D-I but thyrotropin markedly
increased 5'D-I ( p < 0.001 ) ... This TSH induced increase in
5'D-I was
attenuated by
TNF-alpha in a dose dependent manner ( p < 0.001 ) ... Enzyme kinetic analysis demonstrated that thyrotropin increased 5'D-I by increasing Vmax ( p < 0.01 ) without significantly affecting Km. Likewise, TNF-alpha decreased the thyrotropin induced 5'D-I by decreasing Vmax ( p < 0.05 ) but not Km. The effect of TNF-alpha on thyrotropin induced 5'D-I in FRTL-5 cells is probably mediated through post-thyrotropin induced generation of cyclic adenosine monophosphate ( cAMP ) because
TNF-alpha inhibited both dibutyryl cAMP ( p < 0.001 ) and forskolin ( p < 0.001 ) -induced increases in
5'D-I without affecting cAMP generation stimulated by thyrotropin ... In conclusion, we have demonstrated that
TNF-alpha inhibits thyrotropin induced
5'D-I activity in FRTL-5 cells by pathways distal to the generation of cAMP and that TNF-alpha may play a role in the modulation of the production of triiodothyronine by the thyroid gland