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GIT1 — PXN

Pathways - manually collected, often from reviews:

• KEGG Regulation of actin cytoskeleton: GIT1 → PXN (protein-protein, inhibition)
• NCI Pathway Database Alpha4 beta1 integrin signaling events: ARF6/GTP complex () → alpha4/beta1 Integrin/Fibronectin/Paxillin/GIT1 complex (PXN-GIT1-FN1) (modification, collaborate)
  Goldfinger et al., J Cell Biol 2003, Nishiya et al., Nat Cell Biol 2005
  Evidence: mutant phenotype, assay
• NCI Pathway Database Alpha4 beta1 integrin signaling events: GIT1 (GIT1) → alpha4/beta1 Integrin/Fibronectin/Paxillin complex (PXN-FN1) (modification, collaborate)
  Goldfinger et al., J Cell Biol 2003, Nishiya et al., Nat Cell Biol 2005
  Evidence: physical interaction
• NCI Pathway Database Alpha4 beta1 integrin signaling events: GIT1 (GIT1) → alpha4/beta1 Integrin/Fibronectin/Paxillin/GIT1 complex (PXN-GIT1-FN1) (modification, collaborate)
  Goldfinger et al., J Cell Biol 2003, Nishiya et al., Nat Cell Biol 2005
  Evidence: physical interaction
• NCI Pathway Database Alpha4 beta1 integrin signaling events: alpha4/beta1 Integrin/Fibronectin/Paxillin complex (PXN-FN1) → alpha4/beta1 Integrin/Fibronectin/Paxillin/GIT1 complex (PXN-GIT1-FN1) (modification, collaborate)
  Goldfinger et al., J Cell Biol 2003, Nishiya et al., Nat Cell Biol 2005
  Evidence: physical interaction
• NCI Pathway Database Arf6 signaling events: ARF6/GDP complex (ARF6) → alphaIIb/beta3 Integrin/paxillin/GIT1 complex (ITGA2B-ITGB3-PXN-GIT1) (modification, collaborate)
  Nishiya et al., Nat Cell Biol 2005
  Evidence: mutant phenotype
• NCI Pathway Database Arf6 signaling events: alphaIIb/beta3 Integrin complex (ITGA2B-ITGB3) → alphaIIb/beta3 Integrin/paxillin/GIT1 complex (ITGA2B-ITGB3-PXN-GIT1) (modification, activates)
  Nishiya et al., Nat Cell Biol 2005
  Evidence: mutant phenotype
• NCI Pathway Database Arf6 signaling events: None → alphaIIb/beta3 Integrin/paxillin/GIT1 complex (ITGA2B-ITGB3-PXN-GIT1) (modification, collaborate)
  Nishiya et al., Nat Cell Biol 2005
  Evidence: mutant phenotype
• WikiPathways Regulation of Actin Cytoskeleton: GIT1 → EZR/RDX/MSN/PXN (mim-inhibition)

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Bind Interaction: Complex of GIT1-PXN Nishiya et al., Nat Cell Biol 2005
• IRef Bind_translation Interaction: PXN — GIT1 (affinity chromatography technology) Nishiya et al., Nat Cell Biol 2005
• IRef Biogrid Interaction: GIT1 — PXN (physical association, affinity chromatography technology) Ko et al., J Neurosci 2003*
• MIPS CORUM ITGA4-PXN-GIT1 complex: ITGA4-PXN-GIT1 complex complex (GIT1-ITGA4-PXN) Nishiya et al., Nat Cell Biol 2005
• IRef Corum Interaction: Complex of GIT1-PXN-ITGA4 (association, affinity chromatography technology) Nishiya et al., Nat Cell Biol 2005
• IRef Hprd Interaction: GIT1 — PXN (in vivo) Premont et al., J Biol Chem 2000*, Nishiya et al., J Biochem 2002*, Haendeler et al., J Biol Chem 2003*, Frank et al., EMBO J 2006*
• IRef Intact Interaction: GIT1 — PXN (physical association, anti bait coimmunoprecipitation) Frank et al., EMBO J 2006*
• IRef Ophid Interaction: GIT1 — PXN (aggregation, interologs mapping) Brown et al., Bioinformatics 2005

Text-mined interactions from Literome

Zhao et al., Mol Cell Biol 2000 : Overexpression of GIT1 in fibroblasts or epithelial cells causes a loss of paxillin from FCs and stimulates cell motility
Webb et al., Biochem Biophys Res Commun 2006 : Taken together, our results indicate that GIT1 phosphorylation on serine 709 increases its binding to paxillin and regulates protrusive activity in cells
Zhang et al., J Biol Chem 2008 : GIT1 mediates the localization of the p21 activated kinase (PAK) and PAK-interactive exchange factor to focal adhesions, and its activation is regulated by the interaction between its C-terminal paxillin binding domain (PBD) and the LD motifs of paxillin