UCSC In-Silico PCR
 
>chr16:50723164+50723476 313bp AAGAGCACCGCAATCAATTAG GTCTGTCCACACAACCGCTC
AAGAGCACCGCAATCAATTAGtgatgtctgaaatggagcagaccaggaga
gcaccacgaattttgccctccataggttagctcatctctgaggtctttcc
ctgctctgacatacttttgttccatgattacctccagcctggtggggaac
aacattggcagtgtgggtgcccaagccttggcactgatgctggcaaagaa
cgtcatgctagaagaactctggtgagtttgggggattctctgctctgggg
aagtggatcacaatctctgttgatcccctggcctcatccatagGAGCGGT
TGTGTGGACAGAC

Primer Melting Temperatures
  Forward: 59.4 C aagagcaccgcaatcaattag
Reverse: 61.8 C gtctgtccacacaaccgctc
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine