UCSC In-Silico PCR

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UCSC In-Silico PCR

>chr19:17829942-17830401 460bp ACCTGGTCCCAGCATCATAG TGACTTGCCACACAGTGAGAG ACCTGGTCCCAGCATCATAGgccccagtggggaggacgcttcctcacctt tctgaccccttcacggttcaggcagcccctccccgctccatcacagatgg cccctacccccaccacgggtggcccctccccctccacccacggaggctcc tcccccaccacatgcgctcctccttggctccaggagttcctgcggatgat gggatgtgagcgggatgtccccgccctctgccgcctcttggaactgctgg aggagggccagaggctgccggcgcctcctgcctgccctgctgaggtgagc gccgcagggctagcctcagtttcccagtctgtagattgggccggggtctc gggcaagccagctggcgcctgagtctctgtactgagaagaaaggctagag tgtgaggccgatgaggatcctggcccccacttggctactCTCTCACTGTG TGGCAAGTCA

Primer Melting Temperatures


	`Forward: 60.0 C acctggtcccagcatcatag Reverse: 60.1 C tgacttgccacacagtgagag`The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3, the formula by Rychlik W, Spencer WJ and Rhoads RE NAR 1990, which can be activated in Primer3 with PRIMER_TM_FORMULA=0.

Help


	What is chr_alt & chr_fix? Replicating in-Silico PCR results on local machine