UCSC In-Silico PCR

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UCSC In-Silico PCR

>chr1:183570657-183570960 304bp ACTGTCCACGTCTGAAACCAG ACTGAGGGCCACTTGAAGGAG ACTGTCCACGTCTGAAACCAGgctcacgtaagaacaggtctagggcatga gcaaagagggagacccagaagaatggaaacagtgctggcagagcctcaca ccctcctgtccttgattttaggtcgtggcatctgtggtggatcaagacag tttctctgggtttgcccctctgcaaccacaggtaaggcagtcctgacctt ctccatggacctaggtctcgagagctttctgtgaagcattcaattcgaga gactatgtgtgctgagttgcctgattgtaagggCTCCTTCAAGTGGCCCT CAGT

Primer Melting Temperatures


	`Forward: 60.2 C actgtccacgtctgaaaccag Reverse: 63.0 C actgagggccacttgaaggag`The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3, the formula by Rychlik W, Spencer WJ and Rhoads RE NAR 1990, which can be activated in Primer3 with PRIMER_TM_FORMULA=0.

Help


	What is chr_alt & chr_fix? Replicating in-Silico PCR results on local machine