UCSC In-Silico PCR
 
>chr16:50716476+50716848 373bp AGCACAGATGCTGGCACTTC TTGGACAGTGAGCACCAAATC
AGCACAGATGCTGGCACTTCagggatgaatgaaagtctttttgggggatt
tgtagatttttttcttgtcttactagctccattttcaaatgtatttattt
tgtctctttagtttgcgcgataacaatatctcagaccgaggcatctgcaa
gctcattgaatgtgctcttcactgcgagcaattgcagaagttagcgtaag
tcagcctgggctgtggacaatgggctccaagtgccctggtctcaccccag
gtcgtgcagcctgggaagctgtgagtgatgggctggggcaggggctgttt
gcatgatggggggtgcaggtgattcctgcccagaggggaagggcaaccct
ggGATTTGGTGCTCACTGTCCAA

Primer Melting Temperatures
  Forward: 61.6 C agcacagatgctggcacttc
Reverse: 61.1 C ttggacagtgagcaccaaatc
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine