UCSC In-Silico PCR
 
>chr5:35870960+35871596 637bp AGGGTCAAAGTGACTTGCAG CAGAATGTGGCTACGACTTG
AGGGTCAAAGTGACTTGCAGaggagatgaattttaaatactataattatt
tccttggctgccctttagacagaatttatttctttttcttttccagttaa
acctgaggctccttttgacctgagtgtcgtctatcgggaaggagccaatg
actttgtggtgacatttaatacatcacacttgcaaaagaagtatgtaaaa
gttttaatgcacgatgtagcttaccgccaggaaaaggatgaaaacaaatg
gacggtatgtagttcaactacattaataaaataaaaacttatgaatgttt
tctattttgttggcctagtagtgcatttcccctgggagggcccaacaatt
ttgctttcaaaatctaccttctactgaaagaatctcccaatattggcccc
atgaaaacctggatcttccctgatgcatactcttctagctctggttgttt
tcttctgctctaattttggtcttcagaatgtttctacattagtgagttgg
ataacaatatagattgaggccaaattaatcctctgtattcaggggcctca
aaaagtgtcatgtctagtgccactttcataggcaaatcaggcaaaatgta
tatctgcttatgatcacCAAGTCGTAGCCACATTCTG

Primer Melting Temperatures
  Forward: 57.9 C agggtcaaagtgacttgcag
Reverse: 56.9 C cagaatgtggctacgacttg
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine