UCSC In-Silico PCR
 
>chr16:50697005+50697430 426bp AGTGAGGGTCATGGTCTCCAG CCAGAAACAGAGTCAGCACTCC
AGTGAGGGTCATGGTCTCCAGgatgcacaaggctttgtgccagaattgct
tggaattgcctagttctggaaggctggttggccaactctggcctccggct
tttcctttgggaatttcccttgaaggtggggttggtagacagatccaggc
tcaccagtcctgtgccactgggcttttggcgttctgcacaaggcctaccc
gcagatgccatgcctgctcccccagcctaatgggctttgatgggggaaga
gggtggttcagcctctcacgatgaggaggaaagagcaagtgtcctcctcg
gacattctccgggtaagaggagcaggcattgtcccgtcccagcttgatcc
tcagccttctttcatccttggccgcgacatgctcccaggcctggggtcag
atggGGAGTGCTGACTCTGTTTCTGG

Primer Melting Temperatures
  Forward: 61.5 C agtgagggtcatggtctccag
Reverse: 61.4 C ccagaaacagagtcagcactcc
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine