UCSC In-Silico PCR
 
>chr11:118342492-118342896 405bp ATTGTCAATAGGGACGCTAAAGT TCACTCCCATCAGTAATGTCC
ATTGTCAATAGGGACGCTAAAGTtcaggccaccttttccatattctctgc
cagctccctgctcagagatagagcaatttacaccgcttccttcctaccct
acccctagcccacccccactctgaaaatttcccaccatcaacggcagaaa
gcagagaagcagacatcttctagttcctcccccactctcctctttccggt
acctgtgagtcagctaggggagggcagctctcacccaggctgatagttcg
gtgacctggctttatctactggatgagttccgctgggagatggaacatag
cacgtttctctctggcctggtactggctacccttctctcgcaaggtaagg
ctactccaggtgggtgggggaagggacctgagagGGACATTACTGATGGG
AGTGA

Primer Melting Temperatures
  Forward: 58.3 C attgtcaatagggacgctaaagt
Reverse: 57.4 C tcactcccatcagtaatgtcc
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine