UCSC In-Silico PCR
 
>chr5:35875862+35876701 840bp CATGCTGGCAATTCTGTGAC ATCCACATGGTAGAAGCAGG
CATGCTGGCAATTCTGTGACattccctgtcagaaaactctatagacctac
tcctgaactgaacatttgatggtgtgtctctctggtgccatcttaatacc
ctttctcctttttctgtgcagaatttaaatgtgagtttcaatcctgaaag
tttcctggactgccagattcatagggtggatgacattcaagctagagatg
aagtggaaggttttctgcaagatacgtttcctcagcaactagaagaatct
gagaagcagaggcttggaggggatgtgcagagccccaactgcccatctga
ggatgtagtcatcactccagaaagctttggaagagattcatccctcacat
gcctggctgggaatgtcagtgcatgtgacgcccctattctctcctcttcc
aggtccctagactgcagggagagtggcaagaatgggcctcatgtgtacca
ggacctcctgcttagccttgggactacaaacagcacgctgccccctccat
tttctctccaatctggaatcctgacattgaacccagttgctcagggtcag
cccattcttacttccctgggatcaaatcaagaagaagcatatgtcaccat
gtccagcttctaccaaaaccagtgaagtgtaagaaacccagactgaactt
accgtgagcgacaaagatgatttaaaagggaagtctagagttcctagtct
ccctcacagcacagagaagacaaaattagcaaaaccccactacacagtct
gcaagattctgaaacattgctttgaccactcttcctgagttcagtggcac
tcaacatgagtcaagagcatCCTGCTTCTACCATGTGGAT

Primer Melting Temperatures
  Forward: 60.3 C catgctggcaattctgtgac
Reverse: 57.6 C atccacatggtagaagcagg
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine