UCSC In-Silico PCR
 
>chr16:50711367+50712002 636bp CCTGTCTTCTCATGGATGGTG CTCAGATGTCTGGCACTCAGC
CCTGTCTTCTCATGGATGGTGtccaaatgccaccaggaactgttgctgca
ggagggggggtccccaaagaccactacagatatgtacctgctgattctgc
agcattttctgctgcatgccacccccccagactcagcttcccaaggtctg
ggacccagtcttcttcggggccgcctccccaccctcctgcacctgggcag
actggctctgtggggcctgggcatgtgctgctacgtgttctcagcccagc
agctccaggcagcacaggtcagccctgatgacatttctcttggcttcctg
gtgcgtgccaaaggtgtcgtgccagggagtacggcgcccctggaattcct
tcacatcactttccagtgcttctttgccgcgttctacctggcactcagtg
ctgatgtgccaccagctttgctcagacacctcttcaattgtggcaggcca
ggcaactcaccaatggccaggctcctgcccacgatgtgcatccaggcctc
ggagggaaaggacagcagcgtggcagctttgctgcagaaggccgagccgc
acaaccttcagatcacagcagccttcctggcagggctgttgtcccgggag
cactggggcctgctgGCTGAGTGCCAGACATCTGAG

Primer Melting Temperatures
  Forward: 60.5 C cctgtcttctcatggatggtg
Reverse: 61.2 C ctcagatgtctggcactcagc
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine