UCSC In-Silico PCR
 
>chr16:50710458+50711141 684bp CCTTCAGTTATGTCAGCGTCC AAGGTTGAAGAGCAGGGTCTG
CCTTCAGTTATGTCAGCGTCCcccgcagcagcccattgtctggttaggtc
ccgtcttcaccatggtgccaccttcatctgcctcttcttctgccttccag
ctgccacatgcaagaagtatatggccaagctgaggaccacggtgtctgct
cagtctcgcttcctcagtacctatgatggagcagagacgctctgcctgga
ggacatatacacagagaatgtcctggaggtctgggcagatgtgggcatgg
ctggacccccgcagaagagcccagccaccctgggcctggaggagctcttc
agcacccctggccacctcaatgacgatgcggacactgtgctggtggtggg
tgaggcgggcagtggcaagagcacgctcctgcagcggctgcacttgctgt
gggctgcagggcaagacttccaggaatttctctttgtcttcccattcagc
tgccggcagctgcagtgcatggccaaaccactctctgtgcggactctact
ctttgagcactgctgttggcctgatgttggtcaagaagacatcttccagt
tactccttgaccaccctgaccgtgtcctgttaacctttgatggctttgac
gagttcaagttcaggttcacggatcgtgaacgccactgctccccgaccga
ccccacctctgtcCAGACCCTGCTCTTCAACCTT

Primer Melting Temperatures
  Forward: 59.7 C ccttcagttatgtcagcgtcc
Reverse: 60.8 C aaggttgaagagcagggtctg
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine