UCSC In-Silico PCR
 
>chr16:50699375+50700119 745bp CTAGAACCATGGCCAACTCG TTCCTGCCAAGAGTCAAGAGC
CTAGAACCATGGCCAACTCGggttctgctggggctgacttgccctggcct
tccctgaccaccctgcatctggcttctggagaagtcccgcactgaccttg
ttctcctccccaggttgtgaaatgtgctcgcaggaggcttttcaggcaca
gaggagccagctggtcgagctgctggtctcagggtccctggaaggcttcg
agagtgtcctggactggctgctgtcctgggaggtcctctcctgggaggac
tacgagggcttccacctcctgggccagcctctctcccacttggccaggcg
ccttctggacaccgtctggaataagggtacttgggcctgtcagaagctca
tcgcggctgcccaagaagcccaggccgacagccagtcccccaagctgcat
ggctgctgggacccccactcgctccacccagcccgagacctgcagagtca
ccggccagccattgtcaggaggctccacagccatgtggagaacatgctgg
acctggcatgggagcggggtttcgtcagccagtatgaatgtgatgaaatc
aggttgccgatcttcacaccgtcccagagggtgaggcactcctggtgtgc
atcacagagttctcaggaaaggggtgcttagtcaccaagactgatttgtc
ctcatgaagtcagcctgtggggtaacttggtccatgggatttcccctaaa
aaggtagccaggcaggtaaaatttGCTCTTGACTCTTGGCAGGAA

Primer Melting Temperatures
  Forward: 60.6 C ctagaaccatggccaactcg
Reverse: 62.0 C ttcctgccaagagtcaagagc
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine