UCSC In-Silico PCR
 
>chr5:177403155-177403716 562bp GAAGCTGGAACACGGGATTG GTAGGCACCCGGAACGATAC
GAAGCTGGAACACGGGATTGgggttcgggagcagggggcttccccagaac
gcttgtggccaggtctgagagcgctgcctctcccctaccccccccgcagg
cccgcacccgaggatctgacggtggtgctcggccaggaacgccgtaacca
cagctgtgagccgtgccagacgttggccgtgcgctcctaccgcttgcacg
aggccttctcgcccgtcagctaccagcacgacctgggtgcgtgggggcgc
cccgcggggacgggaagagagcttggggccccggcgtccccgcctcacgc
tcctctccgcccgggttagctctgttgcgccttcaggaggatgcggacgg
cagctgcgcgctcctgtcgccttacgttcagccggtgtgcctgccaagcg
gcgccgcgcgaccctccgagaccacgctctgccaggtggccggctggggc
caccagttcgagggtaggcacaactgctaggggcaggggtaggggaggag
acctttgatcactgggttaggcggaagaagcccgcgactttgGTATCGTT
CCGGGTGCCTAC

Primer Melting Temperatures
  Forward: 62.9 C gaagctggaacacgggattg
Reverse: 61.6 C gtaggcacccggaacgatac
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine