UCSC In-Silico PCR
 
>chr16:50729727+50729992 266bp GACTGGCTAACTCCTGCAGTC AATTCTGCCATTCCTCTCTCC
GACTGGCTAACTCCTGCAGTCtctttaactggacagtttcaagaggaaaa
ccaagaatccttgaagctcaccattgtatcttcttttccaggttgtccaa
taactgcatcacctacctaggggcagaagccctcctgcaggcccttgaaa
ggaatgacaccatcctggaagtctggtaaggcccctgggcaggcctgttt
tagctctccgaacctcagtttttctatctgtaaaatggggtgacgGGAGA
GAGGAATGGCAGAATT

Primer Melting Temperatures
  Forward: 59.1 C gactggctaactcctgcagtc
Reverse: 59.7 C aattctgccattcctctctcc
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine