UCSC In-Silico PCR
 
>chr16:85920000+85920241 242bp GCCTAAATGCTACAAGCCCTG AAGCTGCCCATACTTACCTGC
GCCTAAATGCTACAAGCCCTGggcctcctgatcccccagccctgctgctg
cgggagttggaggtcatctcggccttgcttgcaaacaccctctgcctgtg
tcattccagagctgcagcagttctataacagccagggccggcttcctgac
ggcagggtggtgctgtgctttggggaagagtttccggatatggccccctt
gcgctccaaactcattctcgtGCAGGTAAGTATGGGCAGCTT

Primer Melting Temperatures
  Forward: 61.1 C gcctaaatgctacaagccctg
Reverse: 60.6 C aagctgcccatacttacctgc
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine