UCSC In-Silico PCR
 
>chr11:118339009-118339676 668bp GCTGGTGGTAGGTTTAGTCTCA GATCCCAAGCAAGTCAGGAC
GCTGGTGGTAGGTTTAGTCTCAgctgggagttactcttgcaatctcctaa
agactcctagaactttgactgttgaaagaacttgtgtgttcatatcactc
atgcagacttctgagggtgtgggagggtggatctcacagtcccatctgct
aggccattgatgtctctctctggttcttctagctgccgacacacaagctc
tgttgaggaatgaccaggtctatcaggtgagcgttgaggggaaggaggca
ggaatgaagggagggtaagtggggatagagaggctcacactgaatgctgt
ttgcacgtgggaagggtcctactggggagttcattgctgggtgtgactgg
agaggtcaggcaggagctctcatcgtcagggcctgctggggcccttcctt
gaagagttctaaccctgcctcctgaccgttttctcttctcctctcttagc
ccctccgagatcgagatgatgctcagtacagccaccttggaggaaactgg
gctcggaacaagtgaacctgagactggtggcttctagaagcagccattac
caactgtaccttcccttcttgctcagccaataaatatatcctctttcact
cagcaggtgcctgggcttcttaaggctcctgggcaaggcgtgggagttGT
CCTGACTTGCTTGGGATC

Primer Melting Temperatures
  Forward: 58.4 C gctggtggtaggtttagtctca
Reverse: 59.7 C gatcccaagcaagtcaggac
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine