UCSC In-Silico PCR
 
>chr16:50725420+50725713 294bp GGCATGTGAGTTCATCATCTTC TCCACCTAGACCAAGGCAAG
GGCATGTGAGTTCATCATCTTCcataatcaatgttgtatcaactggattt
tctctcttcttctcaccagcctggaggagaaccatctccaggatgaaggt
gtatgttctctcgcagaaggactgaagaaaaattcaagtttgaaaatcct
gaagtaaggaacccataagcaggaaacaggacaataattgctggcctttg
gaaggggcatttctgaataagatctgggccgctctccgctgggctaactc
atgtgaggtggcctggtagaacagCTTGCCTTGGTCTAGGTGGA

Primer Melting Temperatures
  Forward: 59.6 C ggcatgtgagttcatcatcttc
Reverse: 60.2 C tccacctagaccaaggcaag
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine