UCSC In-Silico PCR
 
>chr16:50710928+50711514 587bp GGCCAAACCACTCTCTGTGC GACCTTGGGAAGCTGAGTCTG
GGCCAAACCACTCTCTGTGCggactctactctttgagcactgctgttggc
ctgatgttggtcaagaagacatcttccagttactccttgaccaccctgac
cgtgtcctgttaacctttgatggctttgacgagttcaagttcaggttcac
ggatcgtgaacgccactgctccccgaccgaccccacctctgtccagaccc
tgctcttcaaccttctgcagggcaacctgctgaagaatgcccgcaaggtg
gtgaccagccgtccggccgctgtgtcggcgttcctcaggaagtacatccg
caccgagttcaacctcaagggcttctctgaacagggcatcgagctgtacc
tgaggaagcgccatcatgagcccggggtggcggaccgcctcatccgcctg
ctccaagagacctcagccctgcacggtttgtgccacctgcctgtcttctc
atggatggtgtccaaatgccaccaggaactgttgctgcaggagggggggt
ccccaaagaccactacagatatgtacctgctgattctgcagcattttctg
ctgcatgccaccccccCAGACTCAGCTTCCCAAGGTC

Primer Melting Temperatures
  Forward: 63.2 C ggccaaaccactctctgtgc
Reverse: 61.3 C gaccttgggaagctgagtctg
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine