UCSC In-Silico PCR
 
>chr5:177404707-177405256 550bp GGTTGCTGGATACTCGGAGAC TCCTGCCTCCCTCTCATCTG
GGTTGCTGGATACTCGGAGACttggcatggtcctagactctcctgagacc
actatccctctttgtccccagagaagtgctttgagcctcagcttctccgg
tttttccacaagaatgagatatggtatagaactgagcaagcagctgtggc
cagatgccagtgcaagggtcctgatgcccactgccagcggctggccagcc
agggtgagcagatggttgggaacgggccagggaggagcgtcaggaagaca
ggctggcaggaggccgggtggtgtgccaggaaggagagctctctgggggg
gtctttaggcccaggggtggctcactgcgttccctccccaagcctgccgc
accaacccgtgcctccatgggggtcgctgcctagaggtggagggccaccg
cctgtgccactgcccggtgggctacaccggagccttctgcgacgtgggtg
agtgagggtctggggcaagcagaaggccagcccccaggtgggacgggctt
gccaggaaggaggagggagagtgcggaaagCAGATGAGAGGGAGGCAGGA

Primer Melting Temperatures
  Forward: 61.0 C ggttgctggatactcggagac
Reverse: 62.8 C tcctgcctccctctcatctg
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine