UCSC In-Silico PCR
 
>chr16:85918336+85918873 538bp GTGAGACAGACTGTGCACTTGG TGCAAATGGCACACAGATAGG
GTGAGACAGACTGTGCACTTGGgcaggagggaccctgtatgtctccccgc
agcaccgtcatcgtgtccctcttgtccacagcattctcccagatggtgat
cagcttctactatgggggcaagctggtgggccaggccaccaccacctgcc
ccgagggctgccgcctgtccctgagccagcctgggctgcccggcaccaag
ctgtatgggcccgagggcctggagctggtgcgcttcccgccggccgacgc
catccccagcgagcgacagaggcaggtgacgcggaagctgttcgggcacc
tggagcgcggggtgctgctgcacagcagccggcagggcgtgttcgtcaag
cggctgtgccagggccgcgtgttctgcagcggcaacgccgtggtgtgcaa
aggcaggcccaacaagctggagcgtgatgaggtggtccaggtcttcgaca
ccagccagttcttccgaggtctgtaccgtcgtcaccttgctgcccccaca
tcttagagatcacgcctCCTATCTGTGTGCCATTTGCA

Primer Melting Temperatures
  Forward: 61.0 C gtgagacagactgtgcacttgg
Reverse: 62.0 C tgcaaatggcacacagatagg
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine