UCSC In-Silico PCR
 
>chr5:35860750+35861334 585bp TCATGTCTGCCACAGAGTCT GAAGACACTGGATTGTCTCG
TCATGTCTGCCACAGAGTCTgctattttattaaggtcatgccatatttca
aaaggatgcatttatttgtttcattaacagctgcatgtttgttcctcccc
aggagacttggaagatgcagaactggatgactactcattctcatgctata
gccagttggaagtgaatggatcgcagcactcactgacctgtgcttttgag
gacccagatgtcaacatcaccaatctggaatttgaaatatggtgagggat
ggtggttttaatggttgcttagacatcctctgtctctcttttcatatgct
ctttttaatagccacaaaagaaagaatatgtggcctaattaacaaatgtt
aacatctaaggaattcccaaaggcctcctgaaactccttgtccttcacca
aaaacactcatacaaatctcctctcacggttcagctttcagaccctgaga
ctcagtcaaatgatgctctggatcttggggatcccacatccctcccaact
tcatatcagaatttaaatcctgcgtctcctacaacacttctcaccaaaaa
tctgtttgcccaacaCGAGACAATCCAGTGTCTTC

Primer Melting Temperatures
  Forward: 56.8 C tcatgtctgccacagagtct
Reverse: 55.7 C gaagacactggattgtctcg
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine