UCSC In-Silico PCR
 
>chr16:50722498+50722824 327bp TCTGGGATTGAGTGGTCCTG CTCCATTGCCTAACATTGTGG
TCTGGGATTGAGTGGTCCTGcccctctggctgggactgcagagggaggag
gactgttagttcatgtctagaacacatatcaggtactcactgacactgtc
tgttgactcttttggccttttcagattctggggcaacagagtgggtgacg
agggggcccaggccctggctgaagccttgggtgatcaccagagcttgagg
tggctcaggtaagcttcagagtctatcctgcagttttcttggggagatca
ggtgaagagggaggagctggggccagttctgaaggtctttgaactttatt
tctaccCCACAATGTTAGGCAATGGAG

Primer Melting Temperatures
  Forward: 61.1 C tctgggattgagtggtcctg
Reverse: 60.4 C ctccattgcctaacattgtgg
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine