UCSC In-Silico PCR
 
>chr16:50719823+50720178 356bp TCTTCAATGCTTTCTTCCTGTG CCAAGTATGAAGGTGGCAGTC
TCTTCAATGCTTTCTTCCTGTGtttccctggccagggcacagacggccct
ccttttctgcctgccgctgtgttctctcagcctcctctgtcttcccttcc
aggctggggaataactacatcactgccgcgggagcccaagtgctggccga
ggggctccgaggcaacacctccttgcagttcctggggtaggttggattcc
aggaagagggacctgcatggaggggcttgggacttttgaggatttagggg
caggtgaaactcttcagccaggaggccccagaggcagcccagctccagtg
gggaggacaagccagggagagagtgggcggcccttGACTGCCACCTTCAT
ACTTGG

Primer Melting Temperatures
  Forward: 59.5 C tcttcaatgctttcttcctgtg
Reverse: 59.6 C ccaagtatgaaggtggcagtc
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine