UCSC In-Silico PCR
 
>chr5:177402422-177402845 424bp TGAATTAATGGCACCTCCTCC GCTTGGTCCTCACACACCAG
TGAATTAATGGCACCTCCTCCccttcgggcttgctgcgagagaggaaggg
catgagtgggtttacaagcgcctggagcagctttgtccatcgtccgggcg
gcaagcgttgtcagatggggtgtgaagaaggcgctctgtgttcgcagggg
cggaggaatatgccagcttcctgcaggaggcgcaggtaccgttcctctcc
ctggagcgctgctcagccccggacgtgcacggatcctccatcctccccgg
catgctctgcgcagggttcctcgagggcggcaccgatgcgtgccaggtga
gctcttagcccggttggcgcccttccccgaggccgtcaggcacaaatctc
aggtccacagcgctgagctgcgtgtttccgacccagggtgattccggagg
cccgCTGGTGTGTGAGGACCAAGC

Primer Melting Temperatures
  Forward: 61.2 C tgaattaatggcacctcctcc
Reverse: 61.8 C gcttggtcctcacacaccag
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine