UCSC In-Silico PCR
 
>chr5:177409360-177409681 322bp TGACCAAGGAAGTTGCTCCAC GGCCTCCTAGTCACCTGGAC
TGACCAAGGAAGTTGCTCCACttggctttccacaaacagcctgtgcccca
ccaggctcaggagggcagcttgaccaatctctatttccaagacctttggc
cagtcctattgatctggactcctggataggcagctggaccaacggacgga
tgccatgagggctctgctgctcctggggttcctgctggtgagcttggagt
caacactttcggtgagtgctgtgggaaccaggattgtcccaggattgttc
tggggggtcgctatcacagccatgagccatggcctctgctcatgacctgt
ggGTCCAGGTGACTAGGAGGCC

Primer Melting Temperatures
  Forward: 62.5 C tgaccaaggaagttgctccac
Reverse: 61.1 C ggcctcctagtcacctggac
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine