UCSC In-Silico PCR
 
>chr16:85902919+85903254 336bp TGCAAACCTCTGAGTTTCCTG TCCACTAGTCCATGAGGGAAAG
TGCAAACCTCTGAGTTTCCTGttagcagtttttgggttgctgtgatgaat
gagacaatatccgtaatatcacagcgtgtatttctgtctttccaaggatg
tgtgaccggaatggtggtcggcggcttcgacagtggctgatcgagcagat
tgacagtagcatgtatccaggactgatttgggagaatgaggagaagagca
tgttccggatcccttggaaacacgctggcaagcaagattataatcaggaa
gtggatgcctccatttttaaggtaaagagcccagctcccttccccactgt
gggcacagtgtctcCTTTCCCTCATGGACTAGTGGA

Primer Melting Temperatures
  Forward: 60.4 C tgcaaacctctgagtttcctg
Reverse: 60.5 C tccactagtccatgagggaaag
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine