UCSC In-Silico PCR
 
>chr11:118339738-118340067 330bp TGGAGGAAGGTGTGGCAAG ACACAAACACACTCTCATGCTC
TGGAGGAAGGTGTGGCAAGcccatcaccaggcctttctcccagggtctta
ttgaagtttgggttttggcataagagatctaagggttgccctaggaggca
gaggatggttccctgatcttaaaggctctcaacctctcctctctcctccc
ttcccccacagtgtgccagagctgtgtggagctggatccagccaccgtgg
ctggcatcattgtcactgatgtcattgccactctgctccttgctttggga
gtcttctgctttgctggacatgagactggaaggctgtctgggggttagtg
gaagagcaGAGCATGAGAGTGTGTTTGTGT

Primer Melting Temperatures
  Forward: 62.2 C tggaggaaggtgtggcaag
Reverse: 57.9 C acacaaacacactctcatgctc
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine