UCSC In-Silico PCR
 
>chr16:85911437+85911791 355bp TTCCCAAACCAATGAAGACAC GTGGAGATGCCACTTCCTCAG
TTCCCAAACCAATGAAGACACtcactaacttaatgtttccttaaactcag
catttacagagtagattatggcttcagcaaaggctgtgatgcctccgtgc
catgtgtcatggtgtttgtctggttttctgtaggcaaactaggcgtggca
actgctggctgcgtgaatgaagttacagagatggagtgcggtcgctctga
aatcgacgagctgatcaaggaggtaagcagaggcagcatttcaggggtct
ggccctgccaggaggagtctcatgtcttctggcagggaggggcaccgttc
ttgctgtatgaaggcagccagaccctcgggctcgCTGAGGAAGTGGCATC
TCCAC

Primer Melting Temperatures
  Forward: 59.8 C ttcccaaaccaatgaagacac
Reverse: 62.2 C gtggagatgccacttcctcag
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine