UCSC In-Silico PCR
 
>chr16:50731609+50732293 685bp TTCCCTGCAAGAAACACTGC GATGGATGAGTCGAGACATGG
TTCCCTGCAAGAAACACTGCagctgggccagagagtcagcccatcccagg
catgggtttaaaaagtggaggcttttgtttgaaagccctgctctaatttt
gtcctcactcaaacctctgttcacttgatctgctttaggctccgagggaa
cactttctctctagaggaggttgacaagctcggctgcagggacaccagac
tcttgctttgaagtctccgggaggatgttcgtctcagtttgtttgtgagc
aggctgtgagtttgggccccagaggctgggtgacatgtgttggcagcctc
ttcaaaatgagccctgtcctgcctaaggctgaacttgttttctgggaaca
ccataggtcacctttattctggcagaggagggagcatcagtgccctccag
gatagacttttcccaagcctacttttgccattgacttcttcccaagattc
aatcccaggatgtacaaggacagcccctcctccatagtatgggactggcc
tctgctgatcctcccaggcttccgtgtgggtcagtggggcccatggatgt
gcttgttaactgagtgccttttggtggagaggcccggcctctcacaaaag
accccttaccactgctctgatgaagaggagtacacagaacacataattca
ggaagcagctttccCCATGTCTCGACTCATCCATC

Primer Melting Temperatures
  Forward: 61.4 C ttccctgcaagaaacactgc
Reverse: 60.5 C gatggatgagtcgagacatgg
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine